Physiological and biochemical characteristics of boscalid resistant isolates of Sclerotinia sclerotiorum from asparagus lettuce
Laboratory mutants of Sclerotinia sclerotiorum (Lib) de Bary, resistant to boscalid, have been extensively characterized. However, the resistance situation in the lettuce field remains largely elusive. In this study, among the 172 S. sclerotiorum isolates collected from asparagus lettuce field in Ji...
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Elsevier
2023-12-01
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| Series: | Journal of Integrative Agriculture |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311923003246 |
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| author | Dong-ya SHI Feng-jie LI Zhi-hui ZHANG Qiao-nan XU Ying-ying CAO Jane Ifunanya MBADIANYA Xin LI Jin WANG Chang-jun CHEN |
| author_facet | Dong-ya SHI Feng-jie LI Zhi-hui ZHANG Qiao-nan XU Ying-ying CAO Jane Ifunanya MBADIANYA Xin LI Jin WANG Chang-jun CHEN |
| author_sort | Dong-ya SHI |
| collection | DOAJ |
| description | Laboratory mutants of Sclerotinia sclerotiorum (Lib) de Bary, resistant to boscalid, have been extensively characterized. However, the resistance situation in the lettuce field remains largely elusive. In this study, among the 172 S. sclerotiorum isolates collected from asparagus lettuce field in Jiangsu Province, China, 132 isolates (76.74%) exhibited low-level resistance to boscalid (BosLR), with a discriminatory dose of 5 μg mL−1. In comparison to the boscalid-sensitive (BosS) isolates, most BosLR isolates demonstrated a slightly superior biological fitness, as evidenced by data on mycelial growth, sclerotium production and pathogenicity. Moreover, most BosLR isolates showed comparable levels of oxalic acid (OA) accumulation, increased exopolysaccharide (EPS) content and reduced membrane permeability when compared to the BosS isolates. Nevertheless, their responses to distinct stress factors diverged significantly. Furthermore, the effectiveness of boscalid in controlling BosLR isolates on radish was diminished compared to its efficacy on BosS isolates. Genetic mutations were identified in the SDH genes of BosLR isolates, revealing the existence of three resistant genotypes: I (A11V at SDHB, SDHBA11V), II (Q38R at SDHC, SDHCQ38R) and III (SDHBA11V+SDHCQ38R). Importantly, no cross-resistance was observed between boscalid and other fungicides such as thifluzamide, pydiflumetofen, fluazinam, or tebuconazole. Our molecular docking analysis indicated that the docking total score (DTS) of the type I resistant isolates (1.3993) was lower than that of the sensitive isolates (1.7499), implying a reduced affinity between SDHB and boscalid as a potential mechanism underlying the boscalid resistance in S. sclerotiorum. These findings contribute to an enhanced comprehension of boscalid’s mode of action and furnish valuable insights into the management of boscalid resistance. |
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| institution | Directory Open Access Journal |
| issn | 2095-3119 |
| language | English |
| last_indexed | 2024-03-08T23:12:24Z |
| publishDate | 2023-12-01 |
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| spelling | doaj.art-516bf3f5c1ea45d19243b1cf7a3e99062023-12-15T07:23:49ZengElsevierJournal of Integrative Agriculture2095-31192023-12-01221236943708Physiological and biochemical characteristics of boscalid resistant isolates of Sclerotinia sclerotiorum from asparagus lettuceDong-ya SHI0Feng-jie LI1Zhi-hui ZHANG2Qiao-nan XU3Ying-ying CAO4Jane Ifunanya MBADIANYA5Xin LI6Jin WANG7Chang-jun CHEN8Department of Pesticide Science, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, P.R.China; Jiangsu Pesticide Research Institute Co., Ltd., Nanjing 210046, P.R.ChinaDepartment of Pesticide Science, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, P.R.ChinaDepartment of Pesticide Science, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, P.R.ChinaJiangsu Pesticide Research Institute Co., Ltd., Nanjing 210046, P.R.ChinaDepartment of Pesticide Science, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, P.R.ChinaDepartment of Pesticide Science, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, P.R.ChinaDepartment of Pesticide Science, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, P.R.ChinaDepartment of Pesticide Science, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, P.R.ChinaDepartment of Pesticide Science, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, P.R.China; Correspondence CHEN Chang-junLaboratory mutants of Sclerotinia sclerotiorum (Lib) de Bary, resistant to boscalid, have been extensively characterized. However, the resistance situation in the lettuce field remains largely elusive. In this study, among the 172 S. sclerotiorum isolates collected from asparagus lettuce field in Jiangsu Province, China, 132 isolates (76.74%) exhibited low-level resistance to boscalid (BosLR), with a discriminatory dose of 5 μg mL−1. In comparison to the boscalid-sensitive (BosS) isolates, most BosLR isolates demonstrated a slightly superior biological fitness, as evidenced by data on mycelial growth, sclerotium production and pathogenicity. Moreover, most BosLR isolates showed comparable levels of oxalic acid (OA) accumulation, increased exopolysaccharide (EPS) content and reduced membrane permeability when compared to the BosS isolates. Nevertheless, their responses to distinct stress factors diverged significantly. Furthermore, the effectiveness of boscalid in controlling BosLR isolates on radish was diminished compared to its efficacy on BosS isolates. Genetic mutations were identified in the SDH genes of BosLR isolates, revealing the existence of three resistant genotypes: I (A11V at SDHB, SDHBA11V), II (Q38R at SDHC, SDHCQ38R) and III (SDHBA11V+SDHCQ38R). Importantly, no cross-resistance was observed between boscalid and other fungicides such as thifluzamide, pydiflumetofen, fluazinam, or tebuconazole. Our molecular docking analysis indicated that the docking total score (DTS) of the type I resistant isolates (1.3993) was lower than that of the sensitive isolates (1.7499), implying a reduced affinity between SDHB and boscalid as a potential mechanism underlying the boscalid resistance in S. sclerotiorum. These findings contribute to an enhanced comprehension of boscalid’s mode of action and furnish valuable insights into the management of boscalid resistance.http://www.sciencedirect.com/science/article/pii/S2095311923003246Sclerotinia sclerotiorumboscalidasparagus lettuceSDHBA11VSDHCQ38R |
| spellingShingle | Dong-ya SHI Feng-jie LI Zhi-hui ZHANG Qiao-nan XU Ying-ying CAO Jane Ifunanya MBADIANYA Xin LI Jin WANG Chang-jun CHEN Physiological and biochemical characteristics of boscalid resistant isolates of Sclerotinia sclerotiorum from asparagus lettuce Journal of Integrative Agriculture Sclerotinia sclerotiorum boscalid asparagus lettuce SDHBA11V SDHCQ38R |
| title | Physiological and biochemical characteristics of boscalid resistant isolates of Sclerotinia sclerotiorum from asparagus lettuce |
| title_full | Physiological and biochemical characteristics of boscalid resistant isolates of Sclerotinia sclerotiorum from asparagus lettuce |
| title_fullStr | Physiological and biochemical characteristics of boscalid resistant isolates of Sclerotinia sclerotiorum from asparagus lettuce |
| title_full_unstemmed | Physiological and biochemical characteristics of boscalid resistant isolates of Sclerotinia sclerotiorum from asparagus lettuce |
| title_short | Physiological and biochemical characteristics of boscalid resistant isolates of Sclerotinia sclerotiorum from asparagus lettuce |
| title_sort | physiological and biochemical characteristics of boscalid resistant isolates of sclerotinia sclerotiorum from asparagus lettuce |
| topic | Sclerotinia sclerotiorum boscalid asparagus lettuce SDHBA11V SDHCQ38R |
| url | http://www.sciencedirect.com/science/article/pii/S2095311923003246 |
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