Naphthoquine: A Potent Broad-Spectrum Anti-Coronavirus Drug In Vitro
COVID-19 has spread around the world and caused serious public health and social problems. Although several vaccines have been authorized for emergency use, new effective antiviral drugs are still needed. Some repurposed drugs including Chloroquine, Hydroxychloroquine and Remdesivir were immediately...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-01-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/3/712 |
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| author | Yabin Song Yongqiang Deng Huiqiang Wang Zhuchun Bei Hongjing Gu Hui Zhao Hong Wang Dongna Zhang Likun Xu Baogang Wang Yuhuan Li Hongquan Wang |
| author_facet | Yabin Song Yongqiang Deng Huiqiang Wang Zhuchun Bei Hongjing Gu Hui Zhao Hong Wang Dongna Zhang Likun Xu Baogang Wang Yuhuan Li Hongquan Wang |
| author_sort | Yabin Song |
| collection | DOAJ |
| description | COVID-19 has spread around the world and caused serious public health and social problems. Although several vaccines have been authorized for emergency use, new effective antiviral drugs are still needed. Some repurposed drugs including Chloroquine, Hydroxychloroquine and Remdesivir were immediately used to treat COVID-19 after the pandemic. However, the therapeutic effects of these drugs have not been fully demonstrated in clinical studies. In this paper, we found an antimalarial drug, Naphthoquine, showed good broad-spectrum anti-coronavirus activity. Naphthoquineinhibited HCoV-229E, HCoV-OC43 and SARS-CoV-2 replication in vitro, with IC<sub>50</sub> = 2.05 ± 1.44 μM, 5.83 ± 0.74 μM, and 2.01 ± 0.38 µM, respectively. Time-of-addition assay was also performed to explore at which stage Naphthoquine functions during SARS-CoV-2 replication. The results suggested that Naphthoquine may influence virus entry and post-entry replication. Considering the safety of Naphthoquine was even better than that of Chloroquine, we think Naphthoquine has the potential to be used as a broad-spectrum drug for coronavirus infection. |
| first_indexed | 2024-03-09T23:30:07Z |
| format | Article |
| id | doaj.art-517110766a4c44adbe05db97c77ddb95 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T23:30:07Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-517110766a4c44adbe05db97c77ddb952023-11-23T17:11:15ZengMDPI AGMolecules1420-30492022-01-0127371210.3390/molecules27030712Naphthoquine: A Potent Broad-Spectrum Anti-Coronavirus Drug In VitroYabin Song0Yongqiang Deng1Huiqiang Wang2Zhuchun Bei3Hongjing Gu4Hui Zhao5Hong Wang6Dongna Zhang7Likun Xu8Baogang Wang9Yuhuan Li10Hongquan Wang11State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, ChinaState Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, ChinaCAMS Key Laboratory of Antiviral Drug Research, Beijing Key Laboratory of Antimicrobial Agents, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, ChinaState Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, ChinaState Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, ChinaState Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, ChinaState Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, ChinaState Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, ChinaState Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, ChinaState Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, ChinaCAMS Key Laboratory of Antiviral Drug Research, Beijing Key Laboratory of Antimicrobial Agents, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, ChinaState Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, ChinaCOVID-19 has spread around the world and caused serious public health and social problems. Although several vaccines have been authorized for emergency use, new effective antiviral drugs are still needed. Some repurposed drugs including Chloroquine, Hydroxychloroquine and Remdesivir were immediately used to treat COVID-19 after the pandemic. However, the therapeutic effects of these drugs have not been fully demonstrated in clinical studies. In this paper, we found an antimalarial drug, Naphthoquine, showed good broad-spectrum anti-coronavirus activity. Naphthoquineinhibited HCoV-229E, HCoV-OC43 and SARS-CoV-2 replication in vitro, with IC<sub>50</sub> = 2.05 ± 1.44 μM, 5.83 ± 0.74 μM, and 2.01 ± 0.38 µM, respectively. Time-of-addition assay was also performed to explore at which stage Naphthoquine functions during SARS-CoV-2 replication. The results suggested that Naphthoquine may influence virus entry and post-entry replication. Considering the safety of Naphthoquine was even better than that of Chloroquine, we think Naphthoquine has the potential to be used as a broad-spectrum drug for coronavirus infection.https://www.mdpi.com/1420-3049/27/3/712coronavirusSARS-CoV-2repurposingmalariaNaphthoquine |
| spellingShingle | Yabin Song Yongqiang Deng Huiqiang Wang Zhuchun Bei Hongjing Gu Hui Zhao Hong Wang Dongna Zhang Likun Xu Baogang Wang Yuhuan Li Hongquan Wang Naphthoquine: A Potent Broad-Spectrum Anti-Coronavirus Drug In Vitro Molecules coronavirus SARS-CoV-2 repurposing malaria Naphthoquine |
| title | Naphthoquine: A Potent Broad-Spectrum Anti-Coronavirus Drug In Vitro |
| title_full | Naphthoquine: A Potent Broad-Spectrum Anti-Coronavirus Drug In Vitro |
| title_fullStr | Naphthoquine: A Potent Broad-Spectrum Anti-Coronavirus Drug In Vitro |
| title_full_unstemmed | Naphthoquine: A Potent Broad-Spectrum Anti-Coronavirus Drug In Vitro |
| title_short | Naphthoquine: A Potent Broad-Spectrum Anti-Coronavirus Drug In Vitro |
| title_sort | naphthoquine a potent broad spectrum anti coronavirus drug in vitro |
| topic | coronavirus SARS-CoV-2 repurposing malaria Naphthoquine |
| url | https://www.mdpi.com/1420-3049/27/3/712 |
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