Identification of <i>Fusarium oxysporum</i> Causing Leaf Blight on <i>Dendrobium chrysotoxum</i> in Yunnan Province, China
Leaf-blight disease caused by the <i>Fusarium oxysporum</i> is an emerging problem in <i>Dendrobium chrysotoxum</i> production in China. Symptoms of leaf blight were observed on seedlings of <i>D. chrysotoxum</i> cultivated in a nursery in Ruili City, Yunnan Provi...
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MDPI AG
2024-02-01
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| Series: | Life |
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| Online Access: | https://www.mdpi.com/2075-1729/14/3/285 |
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| author | Jun Yang Waqar Ahmed Jinhao Zhang Shunyu Gao Zhenji Wang Haiyan Yang Xuehui Bai Kai Luo Chengdong Xu Guanghai Ji |
| author_facet | Jun Yang Waqar Ahmed Jinhao Zhang Shunyu Gao Zhenji Wang Haiyan Yang Xuehui Bai Kai Luo Chengdong Xu Guanghai Ji |
| author_sort | Jun Yang |
| collection | DOAJ |
| description | Leaf-blight disease caused by the <i>Fusarium oxysporum</i> is an emerging problem in <i>Dendrobium chrysotoxum</i> production in China. Symptoms of leaf blight were observed on seedlings of <i>D. chrysotoxum</i> cultivated in a nursery in Ruili City, Yunnan Province, China. In this study, we isolated the <i>Fusarium</i> sp. associated with leaf-blight disease of <i>D. chrysotoxum</i> from the diseased seedlings. A pathogenicity test was performed to fulfill Koch’s postulates to confirm the pathogenicity of isolated strains and identified using morphological and molecular techniques. The results revealed that all four isolated <i>Fusarium</i> sp. isolates (DHRL-01~04) produced typical blight symptoms followed by marginal necrosis of leaves on the <i>D. chrysotoxum</i> plants. On the PDA medium, the fungal colony appeared as a white to purplish color with cottony mycelium growth. Microconidia are oval-shaped, whereas macroconidia are sickle-shaped, tapering at both ends with 2–4 septations. The phylogenetic trees were construed based on internal transcribed spacer (<i>ITS</i>), translation elongation factor (<i>EF-1α</i>), and RNA polymerase subunit genes <i>RPB1</i> and <i>RPB2</i> genes, respectively, and blasted against the NCBI database for species confirmation. Based on the NCBI database’s blast results, the isolates showed that more than 99% identify with <i>Fusarium oxysporum</i>. To our knowledge, this is the first comprehensive report on the identification of <i>Fusarium oxysporum</i> as the causal agent of <i>Dendrobium chrysotoxum</i> leaf blight in Yunnan Province, China, based on morphological and molecular characteristics. |
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| spelling | doaj.art-5175d933adb646ddbc5ea0fd100eb6842024-03-27T13:51:02ZengMDPI AGLife2075-17292024-02-0114328510.3390/life14030285Identification of <i>Fusarium oxysporum</i> Causing Leaf Blight on <i>Dendrobium chrysotoxum</i> in Yunnan Province, ChinaJun Yang0Waqar Ahmed1Jinhao Zhang2Shunyu Gao3Zhenji Wang4Haiyan Yang5Xuehui Bai6Kai Luo7Chengdong Xu8Guanghai Ji9College of Resources, Environment and Chemistry, Chuxiong Normal University, Chuxiong 675000, ChinaState Key Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Agricultural University, Kunming 650201, ChinaState Key Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Agricultural University, Kunming 650201, ChinaCollege of Resources, Environment and Chemistry, Chuxiong Normal University, Chuxiong 675000, ChinaCollege of Resources, Environment and Chemistry, Chuxiong Normal University, Chuxiong 675000, ChinaCollege of Resources, Environment and Chemistry, Chuxiong Normal University, Chuxiong 675000, ChinaDehong Insitute of Tropical Agriculture in Yunnan Province, Ruili 678600, ChinaDehong Insitute of Tropical Agriculture in Yunnan Province, Ruili 678600, ChinaCollege of Resources, Environment and Chemistry, Chuxiong Normal University, Chuxiong 675000, ChinaState Key Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Agricultural University, Kunming 650201, ChinaLeaf-blight disease caused by the <i>Fusarium oxysporum</i> is an emerging problem in <i>Dendrobium chrysotoxum</i> production in China. Symptoms of leaf blight were observed on seedlings of <i>D. chrysotoxum</i> cultivated in a nursery in Ruili City, Yunnan Province, China. In this study, we isolated the <i>Fusarium</i> sp. associated with leaf-blight disease of <i>D. chrysotoxum</i> from the diseased seedlings. A pathogenicity test was performed to fulfill Koch’s postulates to confirm the pathogenicity of isolated strains and identified using morphological and molecular techniques. The results revealed that all four isolated <i>Fusarium</i> sp. isolates (DHRL-01~04) produced typical blight symptoms followed by marginal necrosis of leaves on the <i>D. chrysotoxum</i> plants. On the PDA medium, the fungal colony appeared as a white to purplish color with cottony mycelium growth. Microconidia are oval-shaped, whereas macroconidia are sickle-shaped, tapering at both ends with 2–4 septations. The phylogenetic trees were construed based on internal transcribed spacer (<i>ITS</i>), translation elongation factor (<i>EF-1α</i>), and RNA polymerase subunit genes <i>RPB1</i> and <i>RPB2</i> genes, respectively, and blasted against the NCBI database for species confirmation. Based on the NCBI database’s blast results, the isolates showed that more than 99% identify with <i>Fusarium oxysporum</i>. To our knowledge, this is the first comprehensive report on the identification of <i>Fusarium oxysporum</i> as the causal agent of <i>Dendrobium chrysotoxum</i> leaf blight in Yunnan Province, China, based on morphological and molecular characteristics.https://www.mdpi.com/2075-1729/14/3/285Koch’s postulatespathogenicitymorphological characteristicsphylogenetic analysisinternal transcribed spacer (ITS) |
| spellingShingle | Jun Yang Waqar Ahmed Jinhao Zhang Shunyu Gao Zhenji Wang Haiyan Yang Xuehui Bai Kai Luo Chengdong Xu Guanghai Ji Identification of <i>Fusarium oxysporum</i> Causing Leaf Blight on <i>Dendrobium chrysotoxum</i> in Yunnan Province, China Life Koch’s postulates pathogenicity morphological characteristics phylogenetic analysis internal transcribed spacer (ITS) |
| title | Identification of <i>Fusarium oxysporum</i> Causing Leaf Blight on <i>Dendrobium chrysotoxum</i> in Yunnan Province, China |
| title_full | Identification of <i>Fusarium oxysporum</i> Causing Leaf Blight on <i>Dendrobium chrysotoxum</i> in Yunnan Province, China |
| title_fullStr | Identification of <i>Fusarium oxysporum</i> Causing Leaf Blight on <i>Dendrobium chrysotoxum</i> in Yunnan Province, China |
| title_full_unstemmed | Identification of <i>Fusarium oxysporum</i> Causing Leaf Blight on <i>Dendrobium chrysotoxum</i> in Yunnan Province, China |
| title_short | Identification of <i>Fusarium oxysporum</i> Causing Leaf Blight on <i>Dendrobium chrysotoxum</i> in Yunnan Province, China |
| title_sort | identification of i fusarium oxysporum i causing leaf blight on i dendrobium chrysotoxum i in yunnan province china |
| topic | Koch’s postulates pathogenicity morphological characteristics phylogenetic analysis internal transcribed spacer (ITS) |
| url | https://www.mdpi.com/2075-1729/14/3/285 |
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