Distribution of multi-level B cell subsets in thymoma and thymoma-associated myasthenia gravis
Abstract B-cell subsets in peripheral blood (PB) and tumor microenvironment (TME) were evaluated to determine myasthenia gravis (MG) severity in patients with thymoma-associated MG (TMG) and the distribution of B cells in type B TMG. The distribution of mature B cells, including Bm1–Bm5, CD19+ and C...
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| Format: | Article |
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Nature Portfolio
2024-02-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-024-53250-6 |
| _version_ | 1797274573238960128 |
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| author | Peng Zhang Yuxin Liu Si Chen Xinyu Zhang Yuanguo Wang Hui Zhang Jian Li Zhaoyu Yang Kai Xiong Shuning Duan Zeyang Zhang Yan Wang Ping Wang |
| author_facet | Peng Zhang Yuxin Liu Si Chen Xinyu Zhang Yuanguo Wang Hui Zhang Jian Li Zhaoyu Yang Kai Xiong Shuning Duan Zeyang Zhang Yan Wang Ping Wang |
| author_sort | Peng Zhang |
| collection | DOAJ |
| description | Abstract B-cell subsets in peripheral blood (PB) and tumor microenvironment (TME) were evaluated to determine myasthenia gravis (MG) severity in patients with thymoma-associated MG (TMG) and the distribution of B cells in type B TMG. The distribution of mature B cells, including Bm1–Bm5, CD19+ and CD20+ B cells and non-switched (NSMBCs) and switched (SMBCs) memory B cells, were determined in 79 patients with thymoma or TMG. Quantitative relationships between the T and TMG groups and the TMG-low and TMG-high subgroups were determined. NSMBCs and SMBCs were compared in TME and PB. Type B thymoma was more likely to develop into MG, with types B2 and B3 being especially associated with MG worsening. The percentage of CD19+ B cells in PB gradually increased, whereas the percentage of CD20+ B cells and the CD19/CD20 ratio were not altered. The (Bm2 + Bm2′)/(eBm5 + Bm5) index was significantly higher in the TMG-high than in thymoma group. The difference between SMBC/CD19+ and NSMBC/CD19+ B cell ratios was significantly lower in the thymoma than TMG group. NSMBCs assembled around tertiary lymphoid tissue in thymomas of patients with TMG. Few NSMBCs were observed in patients with thymoma alone, with these cells being diffusely distributed. MG severity in patients with TMG can be determined by measuring CD19+ B cells and Bm1-Bm5 in PB. The CD19/CD20 ratio is a marker of disease severity in TMG patients. Differences between NSMBCs and SMBCs in PB and TME of thymomas can synergistically determine MG severity in patients with TMG. |
| first_indexed | 2024-03-07T15:00:19Z |
| format | Article |
| id | doaj.art-5178e28b5ca74492ad243841d158f298 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-07T15:00:19Z |
| publishDate | 2024-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-5178e28b5ca74492ad243841d158f2982024-03-05T19:09:22ZengNature PortfolioScientific Reports2045-23222024-02-0114111110.1038/s41598-024-53250-6Distribution of multi-level B cell subsets in thymoma and thymoma-associated myasthenia gravisPeng Zhang0Yuxin Liu1Si Chen2Xinyu Zhang3Yuanguo Wang4Hui Zhang5Jian Li6Zhaoyu Yang7Kai Xiong8Shuning Duan9Zeyang Zhang10Yan Wang11Ping Wang12Department of Cardiovascular Thoracic Surgery, Tianjin Medical University General HospitalDepartment of Cardiovascular Thoracic Surgery, Tianjin Medical University General HospitalDepartment of Cardiovascular Thoracic Surgery, Tianjin Medical University General HospitalSchool of Medicine, University of DundeeDepartment of Cardiovascular Thoracic Surgery, Tianjin Medical University General HospitalDepartment of Cardiovascular Thoracic Surgery, Tianjin Medical University General HospitalDepartment of Cardiovascular Thoracic Surgery, Tianjin Medical University General HospitalDepartment of Cardiovascular Thoracic Surgery, Tianjin Medical University General HospitalDepartment of Cardiovascular Thoracic Surgery, Tianjin Medical University General HospitalDepartment of Cardiovascular Thoracic Surgery, Tianjin Medical University General HospitalDepartment of Cardiovascular Thoracic Surgery, Tianjin Medical University General HospitalDepartment of Cardiovascular Thoracic Surgery, Tianjin Medical University General HospitalTianjin Ruichuang Biological Technology Co. LtdAbstract B-cell subsets in peripheral blood (PB) and tumor microenvironment (TME) were evaluated to determine myasthenia gravis (MG) severity in patients with thymoma-associated MG (TMG) and the distribution of B cells in type B TMG. The distribution of mature B cells, including Bm1–Bm5, CD19+ and CD20+ B cells and non-switched (NSMBCs) and switched (SMBCs) memory B cells, were determined in 79 patients with thymoma or TMG. Quantitative relationships between the T and TMG groups and the TMG-low and TMG-high subgroups were determined. NSMBCs and SMBCs were compared in TME and PB. Type B thymoma was more likely to develop into MG, with types B2 and B3 being especially associated with MG worsening. The percentage of CD19+ B cells in PB gradually increased, whereas the percentage of CD20+ B cells and the CD19/CD20 ratio were not altered. The (Bm2 + Bm2′)/(eBm5 + Bm5) index was significantly higher in the TMG-high than in thymoma group. The difference between SMBC/CD19+ and NSMBC/CD19+ B cell ratios was significantly lower in the thymoma than TMG group. NSMBCs assembled around tertiary lymphoid tissue in thymomas of patients with TMG. Few NSMBCs were observed in patients with thymoma alone, with these cells being diffusely distributed. MG severity in patients with TMG can be determined by measuring CD19+ B cells and Bm1-Bm5 in PB. The CD19/CD20 ratio is a marker of disease severity in TMG patients. Differences between NSMBCs and SMBCs in PB and TME of thymomas can synergistically determine MG severity in patients with TMG.https://doi.org/10.1038/s41598-024-53250-6 |
| spellingShingle | Peng Zhang Yuxin Liu Si Chen Xinyu Zhang Yuanguo Wang Hui Zhang Jian Li Zhaoyu Yang Kai Xiong Shuning Duan Zeyang Zhang Yan Wang Ping Wang Distribution of multi-level B cell subsets in thymoma and thymoma-associated myasthenia gravis Scientific Reports |
| title | Distribution of multi-level B cell subsets in thymoma and thymoma-associated myasthenia gravis |
| title_full | Distribution of multi-level B cell subsets in thymoma and thymoma-associated myasthenia gravis |
| title_fullStr | Distribution of multi-level B cell subsets in thymoma and thymoma-associated myasthenia gravis |
| title_full_unstemmed | Distribution of multi-level B cell subsets in thymoma and thymoma-associated myasthenia gravis |
| title_short | Distribution of multi-level B cell subsets in thymoma and thymoma-associated myasthenia gravis |
| title_sort | distribution of multi level b cell subsets in thymoma and thymoma associated myasthenia gravis |
| url | https://doi.org/10.1038/s41598-024-53250-6 |
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