Evaluation of the Effect of Topical Pistachio Hull Hydroalcoholic Extract Ointment on Wound Healing in Rat: A Histologic and Planimetric Study
Background and Aim: Pistachio nut and hull have anti-inflammatory, antimicrobial and antioxidant effects. The aim of this study was to investigate the effects of pistachio hull hydroalcoholic extract ointment on wound healing process. Materials and methods: For wound planimetric assessment, 20 healt...
| Main Authors: | , , |
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| Format: | Article |
| Language: | fas |
| Published: |
Kurdistan University of Medical Sciences
2022-08-01
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| Series: | مجله علمی دانشگاه علوم پزشکی کردستان |
| Subjects: | |
| Online Access: | http://sjku.muk.ac.ir/article-1-6726-en.html |
| Summary: | Background and Aim: Pistachio nut and hull have anti-inflammatory, antimicrobial and antioxidant effects. The aim of this study was to investigate the effects of pistachio hull hydroalcoholic extract ointment on wound healing process.
Materials and methods: For wound planimetric assessment, 20 healthy rats were randomly divided into 5 groups of 4, including control group (open wound without any intervention and treatment), positive control group (open wound with basic ointment without extract), and the 3 other groups receiving 1%, 2% and 4% ointments respectively. On the 7th and 14th days, biopsies from wound tissues were taken and after Mason Trichrome staining we evaluated collagen. H&E staining was done for histopathological assessments.
Results: The results showed that wound treatment with pistachio hull ointment in the experimental groups showed a significant increase (P<0.05) in the percentage of wound healing rate. Also, there was a significant difference in the thickness of skin and collagen diameter, and reduction of edema between experimental and control groups (P<0.05). Also we found significant decrease in inflammatory cell count on the 14th day in the groups treated with pistachio hull extract compared to those in the control group (P<0.05), which was indicative of accelerated wound healing in the experimental groups.
Conclusion: According to the results of this study, pistachio hull hydroalcoholic extract ointment potentially possesses healing effects on the wound created in the skin of the rat. |
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| ISSN: | 1560-652X 2345-4040 |