Micropropagation of Chapada Diamantina ornamental bromeliad
ABSTRACT: Sincoraea mucugensis (Wand. & A.A. Conc.) LOUZADA & WAND, an endangered bromeliad, is confined to the central region of the Chapada Diamantina, in the municipality of Mucugê, Brazil. From various researches, it is evident that for the propagation of this species, the in vitro techn...
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| Format: | Article |
| Language: | English |
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Universidade Federal de Santa Maria
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| Series: | Ciência Rural |
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| Online Access: | http://www.scielo.br/pdf/cr/v50n2/1678-4596-cr-50-02-e20180922.pdf |
| _version_ | 1819101746512789504 |
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| author | Andressa Priscila Piancó Santos Lima Alone Lima Brito José Raniere Ferreira de Santana |
| author_facet | Andressa Priscila Piancó Santos Lima Alone Lima Brito José Raniere Ferreira de Santana |
| author_sort | Andressa Priscila Piancó Santos Lima |
| collection | DOAJ |
| description | ABSTRACT: Sincoraea mucugensis (Wand. & A.A. Conc.) LOUZADA & WAND, an endangered bromeliad, is confined to the central region of the Chapada Diamantina, in the municipality of Mucugê, Brazil. From various researches, it is evident that for the propagation of this species, the in vitro technique is a feasible option. However, due to the low multiplication rates reported in various papers, this study aimed to establish a micropropagation protocol of direct organogenesis for S. mucugensis. First, the inoculation of the stem explants was done in MS ½ culture medium which contained different levels of BAP (0.00; 6.66; 8.88; 11.10; 13.20 µM) and NAA (0.00; 2.60; 5.20 µM). These shoots were then subjected to a couple of distinct rooting periods (of 30- and 60-day duration) using activated charcoal; finally, these microplants were transferred to a greenhouse for acclimatization, and covered with transparent plastic cups, as a water loss prevention test method. All the data were submitted to the analysis of variance (ANOVA), and the means were subjected to regression analysis or compared using the Tukey test. The findings revealed that the S. mucugensis stem explants raised in the NAA-rich medium (6.42 to 7.43 shoots/explants) showed high multiplication rates; the shoot rooting was done for 30 days using activated charcoal with the medium. Acclimatization, which was performed by directly exposing the microplants to the ex vitro environment, showed 95% survival rate. |
| first_indexed | 2024-12-22T01:23:34Z |
| format | Article |
| id | doaj.art-517b097b0940446594ce9dedf15cee7d |
| institution | Directory Open Access Journal |
| issn | 1678-4596 |
| language | English |
| last_indexed | 2024-12-22T01:23:34Z |
| publisher | Universidade Federal de Santa Maria |
| record_format | Article |
| series | Ciência Rural |
| spelling | doaj.art-517b097b0940446594ce9dedf15cee7d2022-12-21T18:43:40ZengUniversidade Federal de Santa MariaCiência Rural1678-459610.1590/0103-8478cr20180922Micropropagation of Chapada Diamantina ornamental bromeliadAndressa Priscila Piancó Santos LimaAlone Lima BritoJosé Raniere Ferreira de SantanaABSTRACT: Sincoraea mucugensis (Wand. & A.A. Conc.) LOUZADA & WAND, an endangered bromeliad, is confined to the central region of the Chapada Diamantina, in the municipality of Mucugê, Brazil. From various researches, it is evident that for the propagation of this species, the in vitro technique is a feasible option. However, due to the low multiplication rates reported in various papers, this study aimed to establish a micropropagation protocol of direct organogenesis for S. mucugensis. First, the inoculation of the stem explants was done in MS ½ culture medium which contained different levels of BAP (0.00; 6.66; 8.88; 11.10; 13.20 µM) and NAA (0.00; 2.60; 5.20 µM). These shoots were then subjected to a couple of distinct rooting periods (of 30- and 60-day duration) using activated charcoal; finally, these microplants were transferred to a greenhouse for acclimatization, and covered with transparent plastic cups, as a water loss prevention test method. All the data were submitted to the analysis of variance (ANOVA), and the means were subjected to regression analysis or compared using the Tukey test. The findings revealed that the S. mucugensis stem explants raised in the NAA-rich medium (6.42 to 7.43 shoots/explants) showed high multiplication rates; the shoot rooting was done for 30 days using activated charcoal with the medium. Acclimatization, which was performed by directly exposing the microplants to the ex vitro environment, showed 95% survival rate.http://www.scielo.br/pdf/cr/v50n2/1678-4596-cr-50-02-e20180922.pdfin vitro propagationacclimatizationSincoraea mucugensisNAA |
| spellingShingle | Andressa Priscila Piancó Santos Lima Alone Lima Brito José Raniere Ferreira de Santana Micropropagation of Chapada Diamantina ornamental bromeliad Ciência Rural in vitro propagation acclimatization Sincoraea mucugensis NAA |
| title | Micropropagation of Chapada Diamantina ornamental bromeliad |
| title_full | Micropropagation of Chapada Diamantina ornamental bromeliad |
| title_fullStr | Micropropagation of Chapada Diamantina ornamental bromeliad |
| title_full_unstemmed | Micropropagation of Chapada Diamantina ornamental bromeliad |
| title_short | Micropropagation of Chapada Diamantina ornamental bromeliad |
| title_sort | micropropagation of chapada diamantina ornamental bromeliad |
| topic | in vitro propagation acclimatization Sincoraea mucugensis NAA |
| url | http://www.scielo.br/pdf/cr/v50n2/1678-4596-cr-50-02-e20180922.pdf |
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