Stress induced alterations in the outer membrane of Escherichia coli K- 12 strain
In the present study, gram-negative (Escherichia coli K-12) bacterial biomembrane involvement was studied in the presence of modulating factors such as EDTA, Mg ions and EDTA and Mg' ions in combination. The release of proteins and their involvement during the transport of...
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| Format: | Article |
| Language: | English |
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Action for Sustainable Efficacious Development and Awareness
2009-06-01
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| Series: | Environment Conservation Journal |
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| Online Access: | https://journal.environcj.in/index.php/ecj/article/view/1987 |
| _version_ | 1811166180162928640 |
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| author | Bindu Arora |
| author_facet | Bindu Arora |
| author_sort | Bindu Arora |
| collection | DOAJ |
| description |
In the present study, gram-negative (Escherichia coli K-12) bacterial biomembrane involvement was studied in the presence of modulating factors such as EDTA, Mg ions and EDTA and Mg' ions in combination. The release of proteins and their involvement during the transport of 9-Lactams namely Ceftriaxone and Cefazolin were also studied. The broader applications of Ceftriaxone for pharmaceutical implications offer greater advantage as compared to pre-existing 3-Lactams. Due to the availability of more signal moi.:cules in the membranes there is enhanced toxicity at 5 mM EDTA concentration, and easy entrapment of antibiotics, thus enhanced sensitivity levels. A concentration of 15 mM Mg ions was found to be toxic for E.coli whereas it exhibited luxuriant growth with decreasing Mg' ion concentration under antibiotic stress. On the contrary, when 5 mM • EDTA is treated in combination wnth Mg, it attributed reduced signals available on the membrane surface therefore, reduced drug sensitivity. To identify the involvement of specific proteins and to know the site of proteins released which are directly or indirectly involved in transport of antibiotics across the biological membrane, the protein release was monitored from intact cells, as well as, membrane vesicles derived from E.colt cells and studied upto a level of molecular weight determination and measured by using a high-pressure liquid chromatography (HPLC). The study confirms the induction of certain stress signal proteins from the outer membrane, thereby rendering the bacteria more susceptible to therapy.
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| first_indexed | 2024-04-10T15:48:27Z |
| format | Article |
| id | doaj.art-517d1a22f3324793986fdbf9e1595370 |
| institution | Directory Open Access Journal |
| issn | 0972-3099 2278-5124 |
| language | English |
| last_indexed | 2024-04-10T15:48:27Z |
| publishDate | 2009-06-01 |
| publisher | Action for Sustainable Efficacious Development and Awareness |
| record_format | Article |
| series | Environment Conservation Journal |
| spelling | doaj.art-517d1a22f3324793986fdbf9e15953702023-02-12T03:05:16ZengAction for Sustainable Efficacious Development and AwarenessEnvironment Conservation Journal0972-30992278-51242009-06-01101&210.36953/ECJ.2009.101201Stress induced alterations in the outer membrane of Escherichia coli K- 12 strainBindu Arora0Deptt. ofMicrobiology, Abeda Inamdar Sr. College, Pune In the present study, gram-negative (Escherichia coli K-12) bacterial biomembrane involvement was studied in the presence of modulating factors such as EDTA, Mg ions and EDTA and Mg' ions in combination. The release of proteins and their involvement during the transport of 9-Lactams namely Ceftriaxone and Cefazolin were also studied. The broader applications of Ceftriaxone for pharmaceutical implications offer greater advantage as compared to pre-existing 3-Lactams. Due to the availability of more signal moi.:cules in the membranes there is enhanced toxicity at 5 mM EDTA concentration, and easy entrapment of antibiotics, thus enhanced sensitivity levels. A concentration of 15 mM Mg ions was found to be toxic for E.coli whereas it exhibited luxuriant growth with decreasing Mg' ion concentration under antibiotic stress. On the contrary, when 5 mM • EDTA is treated in combination wnth Mg, it attributed reduced signals available on the membrane surface therefore, reduced drug sensitivity. To identify the involvement of specific proteins and to know the site of proteins released which are directly or indirectly involved in transport of antibiotics across the biological membrane, the protein release was monitored from intact cells, as well as, membrane vesicles derived from E.colt cells and studied upto a level of molecular weight determination and measured by using a high-pressure liquid chromatography (HPLC). The study confirms the induction of certain stress signal proteins from the outer membrane, thereby rendering the bacteria more susceptible to therapy. https://journal.environcj.in/index.php/ecj/article/view/1987High pressure liquid chromatographyCEF- Ceftriaxone SodiumCEZ-Cefazolin SodiumOuter Membrane Proteins- OMPs |
| spellingShingle | Bindu Arora Stress induced alterations in the outer membrane of Escherichia coli K- 12 strain Environment Conservation Journal High pressure liquid chromatography CEF- Ceftriaxone Sodium CEZ-Cefazolin Sodium Outer Membrane Proteins- OMPs |
| title | Stress induced alterations in the outer membrane of Escherichia coli K- 12 strain |
| title_full | Stress induced alterations in the outer membrane of Escherichia coli K- 12 strain |
| title_fullStr | Stress induced alterations in the outer membrane of Escherichia coli K- 12 strain |
| title_full_unstemmed | Stress induced alterations in the outer membrane of Escherichia coli K- 12 strain |
| title_short | Stress induced alterations in the outer membrane of Escherichia coli K- 12 strain |
| title_sort | stress induced alterations in the outer membrane of escherichia coli k 12 strain |
| topic | High pressure liquid chromatography CEF- Ceftriaxone Sodium CEZ-Cefazolin Sodium Outer Membrane Proteins- OMPs |
| url | https://journal.environcj.in/index.php/ecj/article/view/1987 |
| work_keys_str_mv | AT binduarora stressinducedalterationsintheoutermembraneofescherichiacolik12strain |