Comparison of Direct Microscopic Examination, Giemsa, Acridine Orange and Two Culture Methods for Detection of Trichomonas vaginalis
Trichomonas vaginalis (T. vaginalis) is the most prevalent sexually transmitted nonviral pathogen. Direct microscopic examination (DME), cytologic smear, staining methods, culture, serologic and molecular biologic techniques are used for the diagnosis of T. vaginalis infections. Today DME is the mos...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Bilimsel Tip Yayinevi
2000-03-01
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| Series: | Flora Infeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi |
| Subjects: | |
| Online Access: | http://www.floradergisi.org/getFileContent.aspx?op=REDPDF&file_name=2000-5-1-061-066.pdf |
| Summary: | Trichomonas vaginalis (T. vaginalis) is the most prevalent sexually transmitted nonviral pathogen. Direct microscopic examination (DME), cytologic smear, staining methods, culture, serologic and molecular biologic techniques are used for the diagnosis of T. vaginalis infections. Today DME is the most frequently used
technique in clinical practice; culture methods are accepted as gold standard and cytologic smear is used as a screening test.
In this study we compared the incidence of T. vaginalis with DME, Giemsa and acridine orange (AO) stains and modified Diamond (MD) and modified thioglycolate (MT) culture media in 269 women; of whom 150 applied to gynecology clinic for cervicitis complaint and 119 working at night clubs and applied to sexually
transmitted diseases clinic for their routine control. We isolated a total of 34 (34/269, 12.6%) T. vaginalis strains. Sixteen of them (16/150, 10.66%) were isolated from single partnered women and 18 (18/119, 15.2%) strains were isolated from multiple partnered
women. Among the compared methods MD was found to be the most sensitive one (94.1%). The followings were DME with 76.5% and MT with 70.6% sensitivity. Giemsa stain had a 58.8% sensitivity, and AO stain being the least sensitive of the compared methods had a 41.2% sensitivity. All methods had 100% specificity. As a result; although DME is more handy and less expensive, it was found to have low sensitivity. Giemsa and AO stains were not superior to DME. So, patients with negative DME results must be confirmed with a highly sensitive culture method. |
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| ISSN: | 1300-932X 1300-932X |