Comparison of Direct Microscopic Examination, Giemsa, Acridine Orange and Two Culture Methods for Detection of Trichomonas vaginalis
Trichomonas vaginalis (T. vaginalis) is the most prevalent sexually transmitted nonviral pathogen. Direct microscopic examination (DME), cytologic smear, staining methods, culture, serologic and molecular biologic techniques are used for the diagnosis of T. vaginalis infections. Today DME is the mos...
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| Format: | Article |
| Language: | English |
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Bilimsel Tip Yayinevi
2000-03-01
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| Series: | Flora Infeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi |
| Subjects: | |
| Online Access: | http://www.floradergisi.org/getFileContent.aspx?op=REDPDF&file_name=2000-5-1-061-066.pdf |
| _version_ | 1797913254951911424 |
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| author | Ali Kudret ADİLOĞLU Ufuk ÖNDE Nilgün ACAR |
| author_facet | Ali Kudret ADİLOĞLU Ufuk ÖNDE Nilgün ACAR |
| author_sort | Ali Kudret ADİLOĞLU |
| collection | DOAJ |
| description | Trichomonas vaginalis (T. vaginalis) is the most prevalent sexually transmitted nonviral pathogen. Direct microscopic examination (DME), cytologic smear, staining methods, culture, serologic and molecular biologic techniques are used for the diagnosis of T. vaginalis infections. Today DME is the most frequently used
technique in clinical practice; culture methods are accepted as gold standard and cytologic smear is used as a screening test.
In this study we compared the incidence of T. vaginalis with DME, Giemsa and acridine orange (AO) stains and modified Diamond (MD) and modified thioglycolate (MT) culture media in 269 women; of whom 150 applied to gynecology clinic for cervicitis complaint and 119 working at night clubs and applied to sexually
transmitted diseases clinic for their routine control. We isolated a total of 34 (34/269, 12.6%) T. vaginalis strains. Sixteen of them (16/150, 10.66%) were isolated from single partnered women and 18 (18/119, 15.2%) strains were isolated from multiple partnered
women. Among the compared methods MD was found to be the most sensitive one (94.1%). The followings were DME with 76.5% and MT with 70.6% sensitivity. Giemsa stain had a 58.8% sensitivity, and AO stain being the least sensitive of the compared methods had a 41.2% sensitivity. All methods had 100% specificity. As a result; although DME is more handy and less expensive, it was found to have low sensitivity. Giemsa and AO stains were not superior to DME. So, patients with negative DME results must be confirmed with a highly sensitive culture method. |
| first_indexed | 2024-04-10T12:09:02Z |
| format | Article |
| id | doaj.art-517d4de263024f05820e2cd84d992b37 |
| institution | Directory Open Access Journal |
| issn | 1300-932X 1300-932X |
| language | English |
| last_indexed | 2024-04-10T12:09:02Z |
| publishDate | 2000-03-01 |
| publisher | Bilimsel Tip Yayinevi |
| record_format | Article |
| series | Flora Infeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi |
| spelling | doaj.art-517d4de263024f05820e2cd84d992b372023-02-15T16:16:06ZengBilimsel Tip YayineviFlora Infeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi1300-932X1300-932X2000-03-01516166Comparison of Direct Microscopic Examination, Giemsa, Acridine Orange and Two Culture Methods for Detection of Trichomonas vaginalisAli Kudret ADİLOĞLU0Ufuk ÖNDE1Nilgün ACAR2S.B. Ankara Eğitim ve Araştırma Hastanesi, Mikrobiyoloji ve Klinik Mikrobiyoloji Laboratuvarı, ANKARAS.B. Ankara Eğitim ve Araştırma Hastanesi, Mikrobiyoloji ve Klinik Mikrobiyoloji Laboratuvarı, ANKARAS.B. Ankara Eğitim ve Araştırma Hastanesi, Mikrobiyoloji ve Klinik Mikrobiyoloji Laboratuvarı, ANKARATrichomonas vaginalis (T. vaginalis) is the most prevalent sexually transmitted nonviral pathogen. Direct microscopic examination (DME), cytologic smear, staining methods, culture, serologic and molecular biologic techniques are used for the diagnosis of T. vaginalis infections. Today DME is the most frequently used technique in clinical practice; culture methods are accepted as gold standard and cytologic smear is used as a screening test. In this study we compared the incidence of T. vaginalis with DME, Giemsa and acridine orange (AO) stains and modified Diamond (MD) and modified thioglycolate (MT) culture media in 269 women; of whom 150 applied to gynecology clinic for cervicitis complaint and 119 working at night clubs and applied to sexually transmitted diseases clinic for their routine control. We isolated a total of 34 (34/269, 12.6%) T. vaginalis strains. Sixteen of them (16/150, 10.66%) were isolated from single partnered women and 18 (18/119, 15.2%) strains were isolated from multiple partnered women. Among the compared methods MD was found to be the most sensitive one (94.1%). The followings were DME with 76.5% and MT with 70.6% sensitivity. Giemsa stain had a 58.8% sensitivity, and AO stain being the least sensitive of the compared methods had a 41.2% sensitivity. All methods had 100% specificity. As a result; although DME is more handy and less expensive, it was found to have low sensitivity. Giemsa and AO stains were not superior to DME. So, patients with negative DME results must be confirmed with a highly sensitive culture method.http://www.floradergisi.org/getFileContent.aspx?op=REDPDF&file_name=2000-5-1-061-066.pdfTrichomonas vaginalisDirect microscopic examinationCulture |
| spellingShingle | Ali Kudret ADİLOĞLU Ufuk ÖNDE Nilgün ACAR Comparison of Direct Microscopic Examination, Giemsa, Acridine Orange and Two Culture Methods for Detection of Trichomonas vaginalis Flora Infeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi Trichomonas vaginalis Direct microscopic examination Culture |
| title | Comparison of Direct Microscopic Examination, Giemsa, Acridine Orange and Two Culture Methods for Detection of Trichomonas vaginalis |
| title_full | Comparison of Direct Microscopic Examination, Giemsa, Acridine Orange and Two Culture Methods for Detection of Trichomonas vaginalis |
| title_fullStr | Comparison of Direct Microscopic Examination, Giemsa, Acridine Orange and Two Culture Methods for Detection of Trichomonas vaginalis |
| title_full_unstemmed | Comparison of Direct Microscopic Examination, Giemsa, Acridine Orange and Two Culture Methods for Detection of Trichomonas vaginalis |
| title_short | Comparison of Direct Microscopic Examination, Giemsa, Acridine Orange and Two Culture Methods for Detection of Trichomonas vaginalis |
| title_sort | comparison of direct microscopic examination giemsa acridine orange and two culture methods for detection of trichomonas vaginalis |
| topic | Trichomonas vaginalis Direct microscopic examination Culture |
| url | http://www.floradergisi.org/getFileContent.aspx?op=REDPDF&file_name=2000-5-1-061-066.pdf |
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