Protocol for in vitro sonoporation validation using non-targeted microbubbles for human studies of ultrasound-mediated gene delivery
Summary: Microbubbles are currently approved for diagnostic ultrasound imaging and are under evaluation in therapeutic protocols. Here, we present a protocol for in vitro sonoporation validation using non-targeted microbubbles for gene delivery. We describe steps for computational simulation, experi...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-12-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166723006901 |
| _version_ | 1797615918744862720 |
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| author | Nisi Zhang Yutong Guo Josquin Foiret Spencer K. Tumbale Ramasamy Paulmurugan Katherine W. Ferrara |
| author_facet | Nisi Zhang Yutong Guo Josquin Foiret Spencer K. Tumbale Ramasamy Paulmurugan Katherine W. Ferrara |
| author_sort | Nisi Zhang |
| collection | DOAJ |
| description | Summary: Microbubbles are currently approved for diagnostic ultrasound imaging and are under evaluation in therapeutic protocols. Here, we present a protocol for in vitro sonoporation validation using non-targeted microbubbles for gene delivery. We describe steps for computational simulation, experimental calibration, reagent preparation, ultrasound treatment, validation, and gene expression analysis. This protocol uses approved diagnostic microbubbles and parameters that are applicable for human use.For complete details on the use and execution of this protocol, please refer to Bez et al. (2017).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| first_indexed | 2024-03-11T07:33:48Z |
| format | Article |
| id | doaj.art-51814e6ed07247008dae53221f31a339 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-03-11T07:33:48Z |
| publishDate | 2023-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-51814e6ed07247008dae53221f31a3392023-11-17T05:28:10ZengElsevierSTAR Protocols2666-16672023-12-0144102723Protocol for in vitro sonoporation validation using non-targeted microbubbles for human studies of ultrasound-mediated gene deliveryNisi Zhang0Yutong Guo1Josquin Foiret2Spencer K. Tumbale3Ramasamy Paulmurugan4Katherine W. Ferrara5Department of Radiology, Stanford University, Palo Alto, CA 94304, USADepartment of Radiology, Stanford University, Palo Alto, CA 94304, USADepartment of Radiology, Stanford University, Palo Alto, CA 94304, USADepartment of Radiology, Stanford University, Palo Alto, CA 94304, USADepartment of Radiology, Stanford University, Palo Alto, CA 94304, USADepartment of Radiology, Stanford University, Palo Alto, CA 94304, USA; Corresponding authorSummary: Microbubbles are currently approved for diagnostic ultrasound imaging and are under evaluation in therapeutic protocols. Here, we present a protocol for in vitro sonoporation validation using non-targeted microbubbles for gene delivery. We describe steps for computational simulation, experimental calibration, reagent preparation, ultrasound treatment, validation, and gene expression analysis. This protocol uses approved diagnostic microbubbles and parameters that are applicable for human use.For complete details on the use and execution of this protocol, please refer to Bez et al. (2017).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166723006901Cell BiologyFlow CytometryMicroscopyMolecular BiologyGene ExpressionBiotechnology and bioengineering |
| spellingShingle | Nisi Zhang Yutong Guo Josquin Foiret Spencer K. Tumbale Ramasamy Paulmurugan Katherine W. Ferrara Protocol for in vitro sonoporation validation using non-targeted microbubbles for human studies of ultrasound-mediated gene delivery STAR Protocols Cell Biology Flow Cytometry Microscopy Molecular Biology Gene Expression Biotechnology and bioengineering |
| title | Protocol for in vitro sonoporation validation using non-targeted microbubbles for human studies of ultrasound-mediated gene delivery |
| title_full | Protocol for in vitro sonoporation validation using non-targeted microbubbles for human studies of ultrasound-mediated gene delivery |
| title_fullStr | Protocol for in vitro sonoporation validation using non-targeted microbubbles for human studies of ultrasound-mediated gene delivery |
| title_full_unstemmed | Protocol for in vitro sonoporation validation using non-targeted microbubbles for human studies of ultrasound-mediated gene delivery |
| title_short | Protocol for in vitro sonoporation validation using non-targeted microbubbles for human studies of ultrasound-mediated gene delivery |
| title_sort | protocol for in vitro sonoporation validation using non targeted microbubbles for human studies of ultrasound mediated gene delivery |
| topic | Cell Biology Flow Cytometry Microscopy Molecular Biology Gene Expression Biotechnology and bioengineering |
| url | http://www.sciencedirect.com/science/article/pii/S2666166723006901 |
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