Bone marrow mesenchymal stem cells stimulate proliferation and neuronal differentiation of retinal progenitor cells.
During retina development, retinal progenitor cell (RPC) proliferation and differentiation are regulated by complex inter- and intracellular interactions. Bone marrow mesenchymal stem cells (BMSCs) are reported to express a variety of cytokines and neurotrophic factors, which have powerful trophic a...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3786983?pdf=render |
| _version_ | 1819019268532994048 |
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| author | Jing Xia Min Luo Ni Ni Junzhao Chen Yamin Hu Yuan Deng Jing Ji Jibo Zhou Xianqun Fan Ping Gu |
| author_facet | Jing Xia Min Luo Ni Ni Junzhao Chen Yamin Hu Yuan Deng Jing Ji Jibo Zhou Xianqun Fan Ping Gu |
| author_sort | Jing Xia |
| collection | DOAJ |
| description | During retina development, retinal progenitor cell (RPC) proliferation and differentiation are regulated by complex inter- and intracellular interactions. Bone marrow mesenchymal stem cells (BMSCs) are reported to express a variety of cytokines and neurotrophic factors, which have powerful trophic and protective functions for neural tissue-derived cells. Here, we show that the expanded RPC cultures treated with BMSC-derived conditioned medium (CM) which was substantially enriched for bFGF and CNTF, expressed clearly increased levels of nuclear receptor TLX, an essential regulator of neural stem cell (NSC) self-renewal, as well as betacellulin (BTC), an EGF-like protein described as supporting NSC expansion. The BMSC CM- or bFGF-treated RPCs also displayed an obviously enhanced proliferation capability, while BMSC CM-derived bFGF knocked down by anti-bFGF, the effect of BMSC CM on enhancing RPC proliferation was partly reversed. Under differentiation conditions, treatment with BMSC CM or CNTF markedly favoured RPC differentiation towards retinal neurons, including Brn3a-positive retinal ganglion cells (RGCs) and rhodopsin-positive photoreceptors, and clearly diminished retinal glial cell differentiation. These findings demonstrate that BMSCs supported RPC proliferation and neuronal differentiation which may be partly mediated by BMSC CM-derived bFGF and CNTF, reveal potential limitations of RPC culture systems, and suggest a means for optimizing RPC cell fate determination in vitro. |
| first_indexed | 2024-12-21T03:32:37Z |
| format | Article |
| id | doaj.art-51868ca1a60546fea4dc30dc1d01d237 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-21T03:32:37Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-51868ca1a60546fea4dc30dc1d01d2372022-12-21T19:17:25ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0189e7615710.1371/journal.pone.0076157Bone marrow mesenchymal stem cells stimulate proliferation and neuronal differentiation of retinal progenitor cells.Jing XiaMin LuoNi NiJunzhao ChenYamin HuYuan DengJing JiJibo ZhouXianqun FanPing GuDuring retina development, retinal progenitor cell (RPC) proliferation and differentiation are regulated by complex inter- and intracellular interactions. Bone marrow mesenchymal stem cells (BMSCs) are reported to express a variety of cytokines and neurotrophic factors, which have powerful trophic and protective functions for neural tissue-derived cells. Here, we show that the expanded RPC cultures treated with BMSC-derived conditioned medium (CM) which was substantially enriched for bFGF and CNTF, expressed clearly increased levels of nuclear receptor TLX, an essential regulator of neural stem cell (NSC) self-renewal, as well as betacellulin (BTC), an EGF-like protein described as supporting NSC expansion. The BMSC CM- or bFGF-treated RPCs also displayed an obviously enhanced proliferation capability, while BMSC CM-derived bFGF knocked down by anti-bFGF, the effect of BMSC CM on enhancing RPC proliferation was partly reversed. Under differentiation conditions, treatment with BMSC CM or CNTF markedly favoured RPC differentiation towards retinal neurons, including Brn3a-positive retinal ganglion cells (RGCs) and rhodopsin-positive photoreceptors, and clearly diminished retinal glial cell differentiation. These findings demonstrate that BMSCs supported RPC proliferation and neuronal differentiation which may be partly mediated by BMSC CM-derived bFGF and CNTF, reveal potential limitations of RPC culture systems, and suggest a means for optimizing RPC cell fate determination in vitro.http://europepmc.org/articles/PMC3786983?pdf=render |
| spellingShingle | Jing Xia Min Luo Ni Ni Junzhao Chen Yamin Hu Yuan Deng Jing Ji Jibo Zhou Xianqun Fan Ping Gu Bone marrow mesenchymal stem cells stimulate proliferation and neuronal differentiation of retinal progenitor cells. PLoS ONE |
| title | Bone marrow mesenchymal stem cells stimulate proliferation and neuronal differentiation of retinal progenitor cells. |
| title_full | Bone marrow mesenchymal stem cells stimulate proliferation and neuronal differentiation of retinal progenitor cells. |
| title_fullStr | Bone marrow mesenchymal stem cells stimulate proliferation and neuronal differentiation of retinal progenitor cells. |
| title_full_unstemmed | Bone marrow mesenchymal stem cells stimulate proliferation and neuronal differentiation of retinal progenitor cells. |
| title_short | Bone marrow mesenchymal stem cells stimulate proliferation and neuronal differentiation of retinal progenitor cells. |
| title_sort | bone marrow mesenchymal stem cells stimulate proliferation and neuronal differentiation of retinal progenitor cells |
| url | http://europepmc.org/articles/PMC3786983?pdf=render |
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