Therapeutic benefit of mesenchymal stem cells in pregnant rats with angiotensin receptor agonistic autoantibody-induced hypertension: Implications for immunomodulation and cytoprotection
Immunomodulation by mesenchymal stem cells (MSCs) is potentially important for maintaining peripheral tolerance. Preeclampsia may be due to maternal immune rejection of the genetically foreign fetus. This study aimed to investigate the biological function of human umbilical cord–derived mesenchymal...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2017-07-01
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| Series: | Hypertension in Pregnancy |
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| Online Access: | http://dx.doi.org/10.1080/10641955.2017.1329429 |
| _version_ | 1811153897421537280 |
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| author | Dan Zhang Lihua Fu Leilei Wang Lin Lin Lihong Yu Lijun Zhang Tao Shang |
| author_facet | Dan Zhang Lihua Fu Leilei Wang Lin Lin Lihong Yu Lijun Zhang Tao Shang |
| author_sort | Dan Zhang |
| collection | DOAJ |
| description | Immunomodulation by mesenchymal stem cells (MSCs) is potentially important for maintaining peripheral tolerance. Preeclampsia may be due to maternal immune rejection of the genetically foreign fetus. This study aimed to investigate the biological function of human umbilical cord–derived mesenchymal stem cells (HU-MSCs) for the treatment of angiotensin receptor agonistic autoantibody (AT1-AA)-induced hypertension during pregnancy. HU-MSCs were isolated, cultured, and labeled in vitro. AT1-AA and HU-MSCs were administered to pregnant rats. Green fluorescent protein (GFP)–positive HU-MSCs infused in vivo were identified by immunofluorescence. Systolic blood pressure (SBP) was evaluated. The effects of HU-MSCs on fetal weight, kidney burden, and spiral artery remodeling, as well as on the expression of tumor necrosis factor α (TNF-α), interleukin 10 (IL-10), and heme oxygenase 1 (HO-1), were investigated. The SBP levels in the HU-MSC-treated pregnant hypertension rats decreased by gestational day 19. The reduction in fetal weight was largely ameliorated after HU-MSC treatment. Lesion burden in the kidney was attenuated and spiral artery remodeling was improved in HU-MSC-treated pregnant hypertension rats. However, green fluorescent protein (GFP)–labeled cells were sparingly observed in the kidney and placenta. Intravenous infusion of HU-MSCs into AT1-AA-induced rats significantly downregulated serum TNF-α levels and upregulated IL-10 levels, concomitant with increased placenta and mesometrial triangle (MT) HO-1 expression. Taken together, intravenous infusion of HU-MSCs ameliorates AT1-AA-induced pregnancy hypertension, intrauterine growth retardation, kidney impairment, and spiral artery remodeling impairment. Moreover, the potential benefits of HU-MSCs may be attributable to both an interference with the pathogenic immune response and a paracrine cytoprotective action. |
| first_indexed | 2024-03-11T23:46:58Z |
| format | Article |
| id | doaj.art-51870f9e12c64526b2e192e011d81b4a |
| institution | Directory Open Access Journal |
| issn | 1064-1955 1525-6065 |
| language | English |
| last_indexed | 2024-03-11T23:46:58Z |
| publishDate | 2017-07-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Hypertension in Pregnancy |
| spelling | doaj.art-51870f9e12c64526b2e192e011d81b4a2023-09-19T09:24:42ZengTaylor & Francis GroupHypertension in Pregnancy1064-19551525-60652017-07-0136324725810.1080/10641955.2017.13294291329429Therapeutic benefit of mesenchymal stem cells in pregnant rats with angiotensin receptor agonistic autoantibody-induced hypertension: Implications for immunomodulation and cytoprotectionDan Zhang0Lihua Fu1Leilei Wang2Lin Lin3Lihong Yu4Lijun Zhang5Tao Shang6Shenyang Women’s and Children’s HospitalDitan Hospital Capital Medical UniversityShengjing Hospital of China Medical UniversityShenyang Women’s and Children’s HospitalShenyang Women’s and Children’s HospitalShenyang Women’s and Children’s HospitalShengjing Hospital of China Medical UniversityImmunomodulation by mesenchymal stem cells (MSCs) is potentially important for maintaining peripheral tolerance. Preeclampsia may be due to maternal immune rejection of the genetically foreign fetus. This study aimed to investigate the biological function of human umbilical cord–derived mesenchymal stem cells (HU-MSCs) for the treatment of angiotensin receptor agonistic autoantibody (AT1-AA)-induced hypertension during pregnancy. HU-MSCs were isolated, cultured, and labeled in vitro. AT1-AA and HU-MSCs were administered to pregnant rats. Green fluorescent protein (GFP)–positive HU-MSCs infused in vivo were identified by immunofluorescence. Systolic blood pressure (SBP) was evaluated. The effects of HU-MSCs on fetal weight, kidney burden, and spiral artery remodeling, as well as on the expression of tumor necrosis factor α (TNF-α), interleukin 10 (IL-10), and heme oxygenase 1 (HO-1), were investigated. The SBP levels in the HU-MSC-treated pregnant hypertension rats decreased by gestational day 19. The reduction in fetal weight was largely ameliorated after HU-MSC treatment. Lesion burden in the kidney was attenuated and spiral artery remodeling was improved in HU-MSC-treated pregnant hypertension rats. However, green fluorescent protein (GFP)–labeled cells were sparingly observed in the kidney and placenta. Intravenous infusion of HU-MSCs into AT1-AA-induced rats significantly downregulated serum TNF-α levels and upregulated IL-10 levels, concomitant with increased placenta and mesometrial triangle (MT) HO-1 expression. Taken together, intravenous infusion of HU-MSCs ameliorates AT1-AA-induced pregnancy hypertension, intrauterine growth retardation, kidney impairment, and spiral artery remodeling impairment. Moreover, the potential benefits of HU-MSCs may be attributable to both an interference with the pathogenic immune response and a paracrine cytoprotective action.http://dx.doi.org/10.1080/10641955.2017.1329429angiotensin receptor agonistic autoantibodymesenchymal stem cellspreeclampsiaspiral artery remodeling |
| spellingShingle | Dan Zhang Lihua Fu Leilei Wang Lin Lin Lihong Yu Lijun Zhang Tao Shang Therapeutic benefit of mesenchymal stem cells in pregnant rats with angiotensin receptor agonistic autoantibody-induced hypertension: Implications for immunomodulation and cytoprotection Hypertension in Pregnancy angiotensin receptor agonistic autoantibody mesenchymal stem cells preeclampsia spiral artery remodeling |
| title | Therapeutic benefit of mesenchymal stem cells in pregnant rats with angiotensin receptor agonistic autoantibody-induced hypertension: Implications for immunomodulation and cytoprotection |
| title_full | Therapeutic benefit of mesenchymal stem cells in pregnant rats with angiotensin receptor agonistic autoantibody-induced hypertension: Implications for immunomodulation and cytoprotection |
| title_fullStr | Therapeutic benefit of mesenchymal stem cells in pregnant rats with angiotensin receptor agonistic autoantibody-induced hypertension: Implications for immunomodulation and cytoprotection |
| title_full_unstemmed | Therapeutic benefit of mesenchymal stem cells in pregnant rats with angiotensin receptor agonistic autoantibody-induced hypertension: Implications for immunomodulation and cytoprotection |
| title_short | Therapeutic benefit of mesenchymal stem cells in pregnant rats with angiotensin receptor agonistic autoantibody-induced hypertension: Implications for immunomodulation and cytoprotection |
| title_sort | therapeutic benefit of mesenchymal stem cells in pregnant rats with angiotensin receptor agonistic autoantibody induced hypertension implications for immunomodulation and cytoprotection |
| topic | angiotensin receptor agonistic autoantibody mesenchymal stem cells preeclampsia spiral artery remodeling |
| url | http://dx.doi.org/10.1080/10641955.2017.1329429 |
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