Genomic Studies of White-Rot Fungus <i>Cerrena unicolor</i> SP02 Provide Insights into Food Safety Value-Added Utilization of Non-Food Lignocellulosic Biomass
<i>Cerrena unicolor</i> is an ecologically and biotechnologically important wood-degrading basidiomycete with high lignocellulose degrading ability. Biological and genetic investigations are limited in the Cerrena genus and, thus, hinder genetic modification and commercial use. The aim o...
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| Format: | Article |
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MDPI AG
2021-10-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/7/10/835 |
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| author | Zichen Zhang Aabid Manzoor Shah Hassan Mohamed Yao Zhang Nino Tsiklauri Yuanda Song |
| author_facet | Zichen Zhang Aabid Manzoor Shah Hassan Mohamed Yao Zhang Nino Tsiklauri Yuanda Song |
| author_sort | Zichen Zhang |
| collection | DOAJ |
| description | <i>Cerrena unicolor</i> is an ecologically and biotechnologically important wood-degrading basidiomycete with high lignocellulose degrading ability. Biological and genetic investigations are limited in the Cerrena genus and, thus, hinder genetic modification and commercial use. The aim of the present study was to provide a global understanding through genomic and experimental research about lignocellulosic biomass utilization by <i>Cerrena unicolor</i>. In this study, we reported the genome sequence of <i>C. unicolor</i> SP02 by using the Illumina and PacBio 20 platforms to obtain trustworthy assembly and annotation. This is the combinational 2nd and 3rd genome sequencing and assembly of <i>C. unicolor</i> species. The generated genome was 42.79 Mb in size with an N50 contig size of 2.48 Mb, a G + C content of 47.43%, and encoding of 12,277 predicted genes. The genes encoding various lignocellulolytic enzymes including laccase, lignin peroxidase, manganese peroxidase, cytochromes P450, cellulase, xylanase, α-amylase, and pectinase involved in the degradation of lignin, cellulose, xylan, starch, pectin, and chitin that showed the <i>C. unicolor</i> SP02 potentially have a wide range of applications in lignocellulosic biomass conversion. Genome-scale metabolic analysis opened up a valuable resource for a better understanding of carbohydrate-active enzymes (CAZymes) and oxidoreductases that provide insights into the genetic basis and molecular mechanisms for lignocellulosic degradation. The <i>C. unicolor</i> SP02 model can be used for the development of efficient microbial cell factories in lignocellulosic industries. The understanding of the genetic material of <i>C. unicolor</i> SP02 coding for the lignocellulolytic enzymes will significantly benefit us in genetic manipulation, site-directed mutagenesis, and industrial biotechnology. |
| first_indexed | 2024-03-10T06:27:49Z |
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| id | doaj.art-518922c6f52047e482c99f4ac327e899 |
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| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-10T06:27:49Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj.art-518922c6f52047e482c99f4ac327e8992023-11-22T18:47:14ZengMDPI AGJournal of Fungi2309-608X2021-10-0171083510.3390/jof7100835Genomic Studies of White-Rot Fungus <i>Cerrena unicolor</i> SP02 Provide Insights into Food Safety Value-Added Utilization of Non-Food Lignocellulosic BiomassZichen Zhang0Aabid Manzoor Shah1Hassan Mohamed2Yao Zhang3Nino Tsiklauri4Yuanda Song5Colin Ratledge Center of Microbial Lipids, School of Agriculture Engineering and Food Sciences, Shandong University of Technology, Zibo 255000, ChinaColin Ratledge Center of Microbial Lipids, School of Agriculture Engineering and Food Sciences, Shandong University of Technology, Zibo 255000, ChinaColin Ratledge Center of Microbial Lipids, School of Agriculture Engineering and Food Sciences, Shandong University of Technology, Zibo 255000, ChinaColin Ratledge Center of Microbial Lipids, School of Agriculture Engineering and Food Sciences, Shandong University of Technology, Zibo 255000, ChinaDurmishidze Institute of Biochemistry and Biotechnology, Academy of Sciences of Georgia, Tbilisi 0159, GeorgiaColin Ratledge Center of Microbial Lipids, School of Agriculture Engineering and Food Sciences, Shandong University of Technology, Zibo 255000, China<i>Cerrena unicolor</i> is an ecologically and biotechnologically important wood-degrading basidiomycete with high lignocellulose degrading ability. Biological and genetic investigations are limited in the Cerrena genus and, thus, hinder genetic modification and commercial use. The aim of the present study was to provide a global understanding through genomic and experimental research about lignocellulosic biomass utilization by <i>Cerrena unicolor</i>. In this study, we reported the genome sequence of <i>C. unicolor</i> SP02 by using the Illumina and PacBio 20 platforms to obtain trustworthy assembly and annotation. This is the combinational 2nd and 3rd genome sequencing and assembly of <i>C. unicolor</i> species. The generated genome was 42.79 Mb in size with an N50 contig size of 2.48 Mb, a G + C content of 47.43%, and encoding of 12,277 predicted genes. The genes encoding various lignocellulolytic enzymes including laccase, lignin peroxidase, manganese peroxidase, cytochromes P450, cellulase, xylanase, α-amylase, and pectinase involved in the degradation of lignin, cellulose, xylan, starch, pectin, and chitin that showed the <i>C. unicolor</i> SP02 potentially have a wide range of applications in lignocellulosic biomass conversion. Genome-scale metabolic analysis opened up a valuable resource for a better understanding of carbohydrate-active enzymes (CAZymes) and oxidoreductases that provide insights into the genetic basis and molecular mechanisms for lignocellulosic degradation. The <i>C. unicolor</i> SP02 model can be used for the development of efficient microbial cell factories in lignocellulosic industries. The understanding of the genetic material of <i>C. unicolor</i> SP02 coding for the lignocellulolytic enzymes will significantly benefit us in genetic manipulation, site-directed mutagenesis, and industrial biotechnology.https://www.mdpi.com/2309-608X/7/10/835<i>Cerrena unicolor</i>genome sequencinglignocellulolytic enzymesbiomass degradationcarbohydrate-active enzymes (CAZymes) |
| spellingShingle | Zichen Zhang Aabid Manzoor Shah Hassan Mohamed Yao Zhang Nino Tsiklauri Yuanda Song Genomic Studies of White-Rot Fungus <i>Cerrena unicolor</i> SP02 Provide Insights into Food Safety Value-Added Utilization of Non-Food Lignocellulosic Biomass Journal of Fungi <i>Cerrena unicolor</i> genome sequencing lignocellulolytic enzymes biomass degradation carbohydrate-active enzymes (CAZymes) |
| title | Genomic Studies of White-Rot Fungus <i>Cerrena unicolor</i> SP02 Provide Insights into Food Safety Value-Added Utilization of Non-Food Lignocellulosic Biomass |
| title_full | Genomic Studies of White-Rot Fungus <i>Cerrena unicolor</i> SP02 Provide Insights into Food Safety Value-Added Utilization of Non-Food Lignocellulosic Biomass |
| title_fullStr | Genomic Studies of White-Rot Fungus <i>Cerrena unicolor</i> SP02 Provide Insights into Food Safety Value-Added Utilization of Non-Food Lignocellulosic Biomass |
| title_full_unstemmed | Genomic Studies of White-Rot Fungus <i>Cerrena unicolor</i> SP02 Provide Insights into Food Safety Value-Added Utilization of Non-Food Lignocellulosic Biomass |
| title_short | Genomic Studies of White-Rot Fungus <i>Cerrena unicolor</i> SP02 Provide Insights into Food Safety Value-Added Utilization of Non-Food Lignocellulosic Biomass |
| title_sort | genomic studies of white rot fungus i cerrena unicolor i sp02 provide insights into food safety value added utilization of non food lignocellulosic biomass |
| topic | <i>Cerrena unicolor</i> genome sequencing lignocellulolytic enzymes biomass degradation carbohydrate-active enzymes (CAZymes) |
| url | https://www.mdpi.com/2309-608X/7/10/835 |
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