Constitutive Expression of the Xylanase Inhibitor TAXI-III Delays Fusarium Head Blight Symptoms in Durum Wheat Transgenic Plants
Cereals contain xylanase inhibitor (XI) proteins which inhibit microbial xylanases and are considered part of the defense mechanisms to counteract microbial pathogens. Nevertheless, in planta evidence for this role has not been reported yet. Therefore, we produced a number of transgenic plants const...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
The American Phytopathological Society
2013-12-01
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| Series: | Molecular Plant-Microbe Interactions |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI-04-13-0121-R |
| _version_ | 1811286303147294720 |
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| author | Ilaria Moscetti Silvio Tundo Michela Janni Luca Sella Katia Gazzetti Alexandra Tauzin Thierry Giardina Stefania Masci Francesco Favaron Renato D'Ovidio |
| author_facet | Ilaria Moscetti Silvio Tundo Michela Janni Luca Sella Katia Gazzetti Alexandra Tauzin Thierry Giardina Stefania Masci Francesco Favaron Renato D'Ovidio |
| author_sort | Ilaria Moscetti |
| collection | DOAJ |
| description | Cereals contain xylanase inhibitor (XI) proteins which inhibit microbial xylanases and are considered part of the defense mechanisms to counteract microbial pathogens. Nevertheless, in planta evidence for this role has not been reported yet. Therefore, we produced a number of transgenic plants constitutively overexpressing TAXI-III, a member of the TAXI type XI that is induced by pathogen infection. Results showed that TAXI-III endows the transgenic wheat with new inhibition capacities. We also showed that TAXI-III is correctly secreted into the apoplast and possesses the expected inhibition parameters against microbial xylanases. The new inhibition properties of the transgenic plants correlate with a significant delay of Fusarium head blight disease symptoms caused by Fusarium graminearum but do not significantly influence leaf spot symptoms caused by Bipolaris sorokiniana. We showed that this contrasting result can be due to the different capacity of TAXI-III to inhibit the xylanase activity of these two fungal pathogens. These results provide, for the first time, clear evidence in planta that XI are involved in plant defense against fungal pathogens and show the potential to manipulate TAXI-III accumulation to improve wheat resistance against F. graminearum. |
| first_indexed | 2024-04-13T02:57:40Z |
| format | Article |
| id | doaj.art-518a7723b9ba4f43b0e00de440a2cc70 |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-04-13T02:57:40Z |
| publishDate | 2013-12-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-518a7723b9ba4f43b0e00de440a2cc702022-12-22T03:05:34ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062013-12-0126121464147210.1094/MPMI-04-13-0121-RConstitutive Expression of the Xylanase Inhibitor TAXI-III Delays Fusarium Head Blight Symptoms in Durum Wheat Transgenic PlantsIlaria MoscettiSilvio TundoMichela JanniLuca SellaKatia GazzettiAlexandra TauzinThierry GiardinaStefania MasciFrancesco FavaronRenato D'OvidioCereals contain xylanase inhibitor (XI) proteins which inhibit microbial xylanases and are considered part of the defense mechanisms to counteract microbial pathogens. Nevertheless, in planta evidence for this role has not been reported yet. Therefore, we produced a number of transgenic plants constitutively overexpressing TAXI-III, a member of the TAXI type XI that is induced by pathogen infection. Results showed that TAXI-III endows the transgenic wheat with new inhibition capacities. We also showed that TAXI-III is correctly secreted into the apoplast and possesses the expected inhibition parameters against microbial xylanases. The new inhibition properties of the transgenic plants correlate with a significant delay of Fusarium head blight disease symptoms caused by Fusarium graminearum but do not significantly influence leaf spot symptoms caused by Bipolaris sorokiniana. We showed that this contrasting result can be due to the different capacity of TAXI-III to inhibit the xylanase activity of these two fungal pathogens. These results provide, for the first time, clear evidence in planta that XI are involved in plant defense against fungal pathogens and show the potential to manipulate TAXI-III accumulation to improve wheat resistance against F. graminearum.https://apsjournals.apsnet.org/doi/10.1094/MPMI-04-13-0121-R |
| spellingShingle | Ilaria Moscetti Silvio Tundo Michela Janni Luca Sella Katia Gazzetti Alexandra Tauzin Thierry Giardina Stefania Masci Francesco Favaron Renato D'Ovidio Constitutive Expression of the Xylanase Inhibitor TAXI-III Delays Fusarium Head Blight Symptoms in Durum Wheat Transgenic Plants Molecular Plant-Microbe Interactions |
| title | Constitutive Expression of the Xylanase Inhibitor TAXI-III Delays Fusarium Head Blight Symptoms in Durum Wheat Transgenic Plants |
| title_full | Constitutive Expression of the Xylanase Inhibitor TAXI-III Delays Fusarium Head Blight Symptoms in Durum Wheat Transgenic Plants |
| title_fullStr | Constitutive Expression of the Xylanase Inhibitor TAXI-III Delays Fusarium Head Blight Symptoms in Durum Wheat Transgenic Plants |
| title_full_unstemmed | Constitutive Expression of the Xylanase Inhibitor TAXI-III Delays Fusarium Head Blight Symptoms in Durum Wheat Transgenic Plants |
| title_short | Constitutive Expression of the Xylanase Inhibitor TAXI-III Delays Fusarium Head Blight Symptoms in Durum Wheat Transgenic Plants |
| title_sort | constitutive expression of the xylanase inhibitor taxi iii delays fusarium head blight symptoms in durum wheat transgenic plants |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI-04-13-0121-R |
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