Cloning and Expression of hGAD65 Gene in E. Coli BL21

<div>The aim of this study is to construct the hGAD65 gene and to identify the hGAD65 clone by using PCR &amp; RFLP. The samples were derived from normal person &amp; DM patient&rsquo;s blood. Blood DNA was isolated by salting out method and then amplified by PCR with a pair of spe...

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Bibliographic Details
Main Authors: Rista Nikmatu Rohmah, Soraya Widyasari, Aulanni’am ., Fatchiyah .
Format: Article
Language:English
Published: Universitas Gadjah Mada, Yogyakarta 2015-11-01
Series:Indonesian Journal of Biotechnology
Online Access:http://journal.ugm.ac.id/ijbiotech/article/view/7868