Differential Roles of CD36 in Regulating Muscle Insulin Response Depend on Palmitic Acid Load
The possible role of fatty acid translocase (CD36) in the treatment of obesity has gained increasing research interest since researchers recognized its coordinated function in fatty acid uptake and oxidation. However, the effect of CD36 deficiency on intracellular insulin signaling is complex and it...
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MDPI AG
2023-02-01
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| Online Access: | https://www.mdpi.com/2227-9059/11/3/729 |
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| author | Jingyu Sun Yajuan Su Jiajia Chen Duran Qin Yaning Xu Hang Chu Tianfeng Lu Jingmei Dong Lili Qin Weida Li |
| author_facet | Jingyu Sun Yajuan Su Jiajia Chen Duran Qin Yaning Xu Hang Chu Tianfeng Lu Jingmei Dong Lili Qin Weida Li |
| author_sort | Jingyu Sun |
| collection | DOAJ |
| description | The possible role of fatty acid translocase (CD36) in the treatment of obesity has gained increasing research interest since researchers recognized its coordinated function in fatty acid uptake and oxidation. However, the effect of CD36 deficiency on intracellular insulin signaling is complex and its impact may depend on different nutritional stresses. Therefore, we investigated the various effects of CD36 deletion on insulin signaling in C2C12 myotubes with or without palmitic acid (PA) overload. In the present work, we reported the upregulated expression levels of CD36 in the skeletal muscle tissues of obese humans and mice as well as in C2C12 myotubes with PA stimulation. CD36 knockdown using RNA interference showed that insulin signaling was impaired in CD36-deficient C2C12 cells in the absence of PA loading, suggesting that CD36 is essential for the maintenance of insulin action, possibly resulting from increased mitochondrial dysfunction and endoplasmic reticulum (ER) stress; however, CD36 deletion improved insulin signaling in the presence of PA overload due to a reduction in lipid overaccumulation. In conclusion, we identified differential roles of CD36 in regulating muscle insulin response under conditions with and without PA overload, which provides supportive evidence for further research into therapeutic approaches to diabetes. |
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| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-11T06:54:26Z |
| publishDate | 2023-02-01 |
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| series | Biomedicines |
| spelling | doaj.art-518aa4277f254cbcad7f31ede65097012023-11-17T09:44:46ZengMDPI AGBiomedicines2227-90592023-02-0111372910.3390/biomedicines11030729Differential Roles of CD36 in Regulating Muscle Insulin Response Depend on Palmitic Acid LoadJingyu Sun0Yajuan Su1Jiajia Chen2Duran Qin3Yaning Xu4Hang Chu5Tianfeng Lu6Jingmei Dong7Lili Qin8Weida Li9Sports and Health Research Center, Department of Physical Education, Translational Medical Center for Stem Cell Therapy and Institute for Regenerative Medicine, Shanghai East Hospital, School of Life Sciences and Technology, Tongji University, Shanghai 200092, ChinaSports and Health Research Center, Department of Physical Education, Translational Medical Center for Stem Cell Therapy and Institute for Regenerative Medicine, Shanghai East Hospital, School of Life Sciences and Technology, Tongji University, Shanghai 200092, ChinaSports and Health Research Center, Department of Physical Education, Translational Medical Center for Stem Cell Therapy and Institute for Regenerative Medicine, Shanghai East Hospital, School of Life Sciences and Technology, Tongji University, Shanghai 200092, ChinaSports and Health Research Center, Department of Physical Education, Translational Medical Center for Stem Cell Therapy and Institute for Regenerative Medicine, Shanghai East Hospital, School of Life Sciences and Technology, Tongji University, Shanghai 200092, ChinaSports and Health Research Center, Department of Physical Education, Translational Medical Center for Stem Cell Therapy and Institute for Regenerative Medicine, Shanghai East Hospital, School of Life Sciences and Technology, Tongji University, Shanghai 200092, ChinaSports and Health Research Center, Department of Physical Education, Translational Medical Center for Stem Cell Therapy and Institute for Regenerative Medicine, Shanghai East Hospital, School of Life Sciences and Technology, Tongji University, Shanghai 200092, ChinaSports and Health Research Center, Department of Physical Education, Translational Medical Center for Stem Cell Therapy and Institute for Regenerative Medicine, Shanghai East Hospital, School of Life Sciences and Technology, Tongji University, Shanghai 200092, ChinaSports and Health Research Center, Department of Physical Education, Translational Medical Center for Stem Cell Therapy and Institute for Regenerative Medicine, Shanghai East Hospital, School of Life Sciences and Technology, Tongji University, Shanghai 200092, ChinaSports and Health Research Center, Department of Physical Education, Translational Medical Center for Stem Cell Therapy and Institute for Regenerative Medicine, Shanghai East Hospital, School of Life Sciences and Technology, Tongji University, Shanghai 200092, ChinaSports and Health Research Center, Department of Physical Education, Translational Medical Center for Stem Cell Therapy and Institute for Regenerative Medicine, Shanghai East Hospital, School of Life Sciences and Technology, Tongji University, Shanghai 200092, ChinaThe possible role of fatty acid translocase (CD36) in the treatment of obesity has gained increasing research interest since researchers recognized its coordinated function in fatty acid uptake and oxidation. However, the effect of CD36 deficiency on intracellular insulin signaling is complex and its impact may depend on different nutritional stresses. Therefore, we investigated the various effects of CD36 deletion on insulin signaling in C2C12 myotubes with or without palmitic acid (PA) overload. In the present work, we reported the upregulated expression levels of CD36 in the skeletal muscle tissues of obese humans and mice as well as in C2C12 myotubes with PA stimulation. CD36 knockdown using RNA interference showed that insulin signaling was impaired in CD36-deficient C2C12 cells in the absence of PA loading, suggesting that CD36 is essential for the maintenance of insulin action, possibly resulting from increased mitochondrial dysfunction and endoplasmic reticulum (ER) stress; however, CD36 deletion improved insulin signaling in the presence of PA overload due to a reduction in lipid overaccumulation. In conclusion, we identified differential roles of CD36 in regulating muscle insulin response under conditions with and without PA overload, which provides supportive evidence for further research into therapeutic approaches to diabetes.https://www.mdpi.com/2227-9059/11/3/729CD36insulin signalingmitochondrial dysfunctionER stressC2C12 myotubes |
| spellingShingle | Jingyu Sun Yajuan Su Jiajia Chen Duran Qin Yaning Xu Hang Chu Tianfeng Lu Jingmei Dong Lili Qin Weida Li Differential Roles of CD36 in Regulating Muscle Insulin Response Depend on Palmitic Acid Load Biomedicines CD36 insulin signaling mitochondrial dysfunction ER stress C2C12 myotubes |
| title | Differential Roles of CD36 in Regulating Muscle Insulin Response Depend on Palmitic Acid Load |
| title_full | Differential Roles of CD36 in Regulating Muscle Insulin Response Depend on Palmitic Acid Load |
| title_fullStr | Differential Roles of CD36 in Regulating Muscle Insulin Response Depend on Palmitic Acid Load |
| title_full_unstemmed | Differential Roles of CD36 in Regulating Muscle Insulin Response Depend on Palmitic Acid Load |
| title_short | Differential Roles of CD36 in Regulating Muscle Insulin Response Depend on Palmitic Acid Load |
| title_sort | differential roles of cd36 in regulating muscle insulin response depend on palmitic acid load |
| topic | CD36 insulin signaling mitochondrial dysfunction ER stress C2C12 myotubes |
| url | https://www.mdpi.com/2227-9059/11/3/729 |
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