Biodegradation and Metabolic Pathway of the Neonicotinoid Insecticide Thiamethoxam by <i>Labrys portucalensis</i> F11
Thiamethoxam (TMX) is an effective neonicotinoid insecticide. However, its widespread use is detrimental to non-targeted organisms and water systems. This study investigates the biodegradation of this insecticide by <i>Labrys portucalensis</i> F11. After 30 days of incubation in mineral...
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MDPI AG
2022-11-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/22/14326 |
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| author | Oumeima Boufercha Ana R. Monforte Allaoueddine Boudemagh António C. Ferreira Paula M. L. Castro Irina S. Moreira |
| author_facet | Oumeima Boufercha Ana R. Monforte Allaoueddine Boudemagh António C. Ferreira Paula M. L. Castro Irina S. Moreira |
| author_sort | Oumeima Boufercha |
| collection | DOAJ |
| description | Thiamethoxam (TMX) is an effective neonicotinoid insecticide. However, its widespread use is detrimental to non-targeted organisms and water systems. This study investigates the biodegradation of this insecticide by <i>Labrys portucalensis</i> F11. After 30 days of incubation in mineral salt medium, <i>L. portucalensis</i> F11 was able to remove 41%, 35% and 100% of a supplied amount of TMX (10.8 mg L<sup>−1</sup>) provided as the sole carbon and nitrogen source, the sole carbon and sulfur source and as the sole carbon source, respectively. Periodic feeding with sodium acetate as the supplementary carbon source resulted in faster degradation of TMX (10.8 mg L<sup>−1</sup>); more than 90% was removed in 3 days. The detection and identification of biodegradation intermediates was performed by UPLC-QTOF/MS/MS. The chemical structure of 12 metabolites is proposed. Nitro reduction, oxadiazine ring cleavage and dechlorination are the main degradation pathways proposed. After biodegradation, toxicity was removed as indicated using <i>Aliivibrio fischeri</i> and by assessing the synthesis of an inducible β-galactosidase by an <i>E. coli</i> mutant (Toxi-Chromo test). <i>L. portucalensis</i> F11 was able to degrade TMX under different conditions and could be effective in bioremediation strategies. |
| first_indexed | 2024-03-09T18:15:37Z |
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| language | English |
| last_indexed | 2024-03-09T18:15:37Z |
| publishDate | 2022-11-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-519000ad623a4ba4ba4731604a6b75ff2023-11-24T08:42:19ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-11-0123221432610.3390/ijms232214326Biodegradation and Metabolic Pathway of the Neonicotinoid Insecticide Thiamethoxam by <i>Labrys portucalensis</i> F11Oumeima Boufercha0Ana R. Monforte1Allaoueddine Boudemagh2António C. Ferreira3Paula M. L. Castro4Irina S. Moreira5Laboratory of Molecular and Cellular Biology, University of Brothers Mentouri, Constantine 1, Chaâbat Erssas Campus, Ain El Bey Road, Constantine 25000, AlgeriaCBQF—Centro de Biotecnologia e Química Fina, Laboratório Associado, Escola Superior de Biotecnologia, Universidade Católica Portuguesa, Rua Diogo Botelho 1327, 4169-005 Porto, PortugalDepartment of Microbiology, Faculty of Natural and Life Sciences, University of Brothers Mentouri, Constantine 1, Ain El Bey Road, Constantine 25000, AlgeriaCBQF—Centro de Biotecnologia e Química Fina, Laboratório Associado, Escola Superior de Biotecnologia, Universidade Católica Portuguesa, Rua Diogo Botelho 1327, 4169-005 Porto, PortugalCBQF—Centro de Biotecnologia e Química Fina, Laboratório Associado, Escola Superior de Biotecnologia, Universidade Católica Portuguesa, Rua Diogo Botelho 1327, 4169-005 Porto, PortugalCBQF—Centro de Biotecnologia e Química Fina, Laboratório Associado, Escola Superior de Biotecnologia, Universidade Católica Portuguesa, Rua Diogo Botelho 1327, 4169-005 Porto, PortugalThiamethoxam (TMX) is an effective neonicotinoid insecticide. However, its widespread use is detrimental to non-targeted organisms and water systems. This study investigates the biodegradation of this insecticide by <i>Labrys portucalensis</i> F11. After 30 days of incubation in mineral salt medium, <i>L. portucalensis</i> F11 was able to remove 41%, 35% and 100% of a supplied amount of TMX (10.8 mg L<sup>−1</sup>) provided as the sole carbon and nitrogen source, the sole carbon and sulfur source and as the sole carbon source, respectively. Periodic feeding with sodium acetate as the supplementary carbon source resulted in faster degradation of TMX (10.8 mg L<sup>−1</sup>); more than 90% was removed in 3 days. The detection and identification of biodegradation intermediates was performed by UPLC-QTOF/MS/MS. The chemical structure of 12 metabolites is proposed. Nitro reduction, oxadiazine ring cleavage and dechlorination are the main degradation pathways proposed. After biodegradation, toxicity was removed as indicated using <i>Aliivibrio fischeri</i> and by assessing the synthesis of an inducible β-galactosidase by an <i>E. coli</i> mutant (Toxi-Chromo test). <i>L. portucalensis</i> F11 was able to degrade TMX under different conditions and could be effective in bioremediation strategies.https://www.mdpi.com/1422-0067/23/22/14326thiamethoxam<i>Labrys portucalensis</i> F11biodegradationmetabolitestoxicity |
| spellingShingle | Oumeima Boufercha Ana R. Monforte Allaoueddine Boudemagh António C. Ferreira Paula M. L. Castro Irina S. Moreira Biodegradation and Metabolic Pathway of the Neonicotinoid Insecticide Thiamethoxam by <i>Labrys portucalensis</i> F11 International Journal of Molecular Sciences thiamethoxam <i>Labrys portucalensis</i> F11 biodegradation metabolites toxicity |
| title | Biodegradation and Metabolic Pathway of the Neonicotinoid Insecticide Thiamethoxam by <i>Labrys portucalensis</i> F11 |
| title_full | Biodegradation and Metabolic Pathway of the Neonicotinoid Insecticide Thiamethoxam by <i>Labrys portucalensis</i> F11 |
| title_fullStr | Biodegradation and Metabolic Pathway of the Neonicotinoid Insecticide Thiamethoxam by <i>Labrys portucalensis</i> F11 |
| title_full_unstemmed | Biodegradation and Metabolic Pathway of the Neonicotinoid Insecticide Thiamethoxam by <i>Labrys portucalensis</i> F11 |
| title_short | Biodegradation and Metabolic Pathway of the Neonicotinoid Insecticide Thiamethoxam by <i>Labrys portucalensis</i> F11 |
| title_sort | biodegradation and metabolic pathway of the neonicotinoid insecticide thiamethoxam by i labrys portucalensis i f11 |
| topic | thiamethoxam <i>Labrys portucalensis</i> F11 biodegradation metabolites toxicity |
| url | https://www.mdpi.com/1422-0067/23/22/14326 |
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