Development of Gelatin-Alginate-TiO2-SOD Biosensor for the Detection of Superoxide Radicals
In this work, a biosensor that uses gelatin and alginate hydrogels in addition to titanium dioxide (TiO2) nanoparticles (NPs) as sensor matrix was developed in order to detect superoxide radicals (O2•-), which play role in carcinogenesis when present in excess levels. Parameters affecting the perfor...
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| Format: | Article |
| Language: | English |
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Turkish Chemical Society
2020-06-01
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| Series: | Journal of the Turkish Chemical Society, Section A: Chemistry |
| Subjects: | |
| Online Access: | https://dergipark.org.tr/tr/pub/jotcsa/issue/52506/646433 |
| _version_ | 1797913992299020288 |
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| author | Utku KARAKAYA Burak DERKUŞ Emel EMREGUL |
| author_facet | Utku KARAKAYA Burak DERKUŞ Emel EMREGUL |
| author_sort | Utku KARAKAYA |
| collection | DOAJ |
| description | In this work, a biosensor that uses gelatin and alginate hydrogels in addition to titanium dioxide (TiO2) nanoparticles (NPs) as sensor matrix was developed in order to detect superoxide radicals (O2•-), which play role in carcinogenesis when present in excess levels. Parameters affecting the performance of the biosensor such as amount of gelatin-alginate ratio, amount of TiO2 NPs, concentration of SOD enzymes and glutaraldehyde cross-linker were investigated. Electrochemical Impedance Spectroscopy (EIS) and chronoamperometry were used as electrochemical technique for the development of biosensor as well as characterisation steps. The developed biosensor exhibited two linear ranges between 0.0009 mM – 0.125 mM and 0.25 mM – 2 mM which were utilized as calibration curves. Detection limit of the biosensor was found 0.9 μM, which was at appropriate level for the detection of O2•- in tumour samples. Finally, the constructed biosensor showed significant analytical performance such as high selection for O2•-, low detection limit, and long-term stability. |
| first_indexed | 2024-04-10T12:19:58Z |
| format | Article |
| id | doaj.art-51931ec857b0476a9cbccdb272f77857 |
| institution | Directory Open Access Journal |
| issn | 2149-0120 |
| language | English |
| last_indexed | 2024-04-10T12:19:58Z |
| publishDate | 2020-06-01 |
| publisher | Turkish Chemical Society |
| record_format | Article |
| series | Journal of the Turkish Chemical Society, Section A: Chemistry |
| spelling | doaj.art-51931ec857b0476a9cbccdb272f778572023-02-15T16:15:29ZengTurkish Chemical SocietyJournal of the Turkish Chemical Society, Section A: Chemistry2149-01202020-06-017257158010.18596/jotcsa.646433Development of Gelatin-Alginate-TiO2-SOD Biosensor for the Detection of Superoxide RadicalsUtku KARAKAYA0https://orcid.org/0000-0002-0970-7622Burak DERKUŞ1https://orcid.org/0000-0001-5558-0995Emel EMREGUL2https://orcid.org/0000-0002-7892-6730ANKARA UNIVERSITYESKISEHIR OSMANGAZI UNIVERSITYANKARA UNIVERSITYIn this work, a biosensor that uses gelatin and alginate hydrogels in addition to titanium dioxide (TiO2) nanoparticles (NPs) as sensor matrix was developed in order to detect superoxide radicals (O2•-), which play role in carcinogenesis when present in excess levels. Parameters affecting the performance of the biosensor such as amount of gelatin-alginate ratio, amount of TiO2 NPs, concentration of SOD enzymes and glutaraldehyde cross-linker were investigated. Electrochemical Impedance Spectroscopy (EIS) and chronoamperometry were used as electrochemical technique for the development of biosensor as well as characterisation steps. The developed biosensor exhibited two linear ranges between 0.0009 mM – 0.125 mM and 0.25 mM – 2 mM which were utilized as calibration curves. Detection limit of the biosensor was found 0.9 μM, which was at appropriate level for the detection of O2•- in tumour samples. Finally, the constructed biosensor showed significant analytical performance such as high selection for O2•-, low detection limit, and long-term stability.https://dergipark.org.tr/tr/pub/jotcsa/issue/52506/646433biyosensörbiosensorbiopolymerbiyopolimer |
| spellingShingle | Utku KARAKAYA Burak DERKUŞ Emel EMREGUL Development of Gelatin-Alginate-TiO2-SOD Biosensor for the Detection of Superoxide Radicals Journal of the Turkish Chemical Society, Section A: Chemistry biyosensör biosensor biopolymer biyopolimer |
| title | Development of Gelatin-Alginate-TiO2-SOD Biosensor for the Detection of Superoxide Radicals |
| title_full | Development of Gelatin-Alginate-TiO2-SOD Biosensor for the Detection of Superoxide Radicals |
| title_fullStr | Development of Gelatin-Alginate-TiO2-SOD Biosensor for the Detection of Superoxide Radicals |
| title_full_unstemmed | Development of Gelatin-Alginate-TiO2-SOD Biosensor for the Detection of Superoxide Radicals |
| title_short | Development of Gelatin-Alginate-TiO2-SOD Biosensor for the Detection of Superoxide Radicals |
| title_sort | development of gelatin alginate tio2 sod biosensor for the detection of superoxide radicals |
| topic | biyosensör biosensor biopolymer biyopolimer |
| url | https://dergipark.org.tr/tr/pub/jotcsa/issue/52506/646433 |
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