Recovery of Bacillus sphaericus spores by flocculation/sedimentation and flotation
The aim of this work was use flocculation/sedimentation and flotation for recovery of spores of the Bacillus sphaericus. Microorganism was produced batchwise using culture medium based skimmed milk, corn steep liquor and mineral salts. The best results of flocculation were obtained using CaCl2.2H2O,...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Instituto de Tecnologia do Paraná (Tecpar)
2005-06-01
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| Series: | Brazilian Archives of Biology and Technology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000400008 |
| _version_ | 1819119702659563520 |
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| author | Christine Lamenha Luna Carlos Edison Lopes Giulio Massarani |
| author_facet | Christine Lamenha Luna Carlos Edison Lopes Giulio Massarani |
| author_sort | Christine Lamenha Luna |
| collection | DOAJ |
| description | The aim of this work was use flocculation/sedimentation and flotation for recovery of spores of the Bacillus sphaericus. Microorganism was produced batchwise using culture medium based skimmed milk, corn steep liquor and mineral salts. The best results of flocculation were obtained using CaCl2.2H2O, FeCl3.6H2O, Al2(SO4)3 and tannin as flocculating agents, with optimal flocculation concentrations of 1,500, 3,000, 2,000 and 1,700ppm, respectively. Flocculent suspensions were characterized based on floc diameter and density. Settling tests were performed in batch at different concentrations of the cellular suspensions and revealed high recovery of the solids in suspension in all cases. Flotation tests were accomplished using a mechanical agitated flotation cell and the process was favoured by the increase of the system agitation and for the presence of a cationic collector.<br>O trabalho aborda a recuperação de esporos da bactéria Bacillus sphaericus por floculação/sedimentação e flotação. O microrganismo foi produzido em batelada, utilizando-se meio de cultivo à base de leite desnatado, milhocina e sais minerais. Os melhores resultados de floculação foram obtidos com os floculantes CaCl2.2H2O, FeCl3.6H2O, Al2(SO4)3 e tanino, com concentrações ótimas de 1.500, 3.000, 2.000 e 1.700ppm, respectivamente. Os sistemas floculentos foram caracterizados através da determinação da densidade e do diâmetro médio dos flocos. Testes de sedimentação em batelada a diferentes concentrações das suspensões celulares revelaram elevados índices de recuperação dos sólidos em suspensão em todos os casos. Os ensaios de flotação foram realizados em célula de flotação mecânica, e o processo foi favorecido pelo aumento da agitação do sistema e pela presença de um coletor catiônico. |
| first_indexed | 2024-12-22T06:08:58Z |
| format | Article |
| id | doaj.art-51986f5c46784961bf7ae6d19a376df6 |
| institution | Directory Open Access Journal |
| issn | 1516-8913 1678-4324 |
| language | English |
| last_indexed | 2024-12-22T06:08:58Z |
| publishDate | 2005-06-01 |
| publisher | Instituto de Tecnologia do Paraná (Tecpar) |
| record_format | Article |
| series | Brazilian Archives of Biology and Technology |
| spelling | doaj.art-51986f5c46784961bf7ae6d19a376df62022-12-21T18:36:18ZengInstituto de Tecnologia do Paraná (Tecpar)Brazilian Archives of Biology and Technology1516-89131678-43242005-06-0148spe617010.1590/S1516-89132005000400008Recovery of Bacillus sphaericus spores by flocculation/sedimentation and flotationChristine Lamenha LunaCarlos Edison LopesGiulio MassaraniThe aim of this work was use flocculation/sedimentation and flotation for recovery of spores of the Bacillus sphaericus. Microorganism was produced batchwise using culture medium based skimmed milk, corn steep liquor and mineral salts. The best results of flocculation were obtained using CaCl2.2H2O, FeCl3.6H2O, Al2(SO4)3 and tannin as flocculating agents, with optimal flocculation concentrations of 1,500, 3,000, 2,000 and 1,700ppm, respectively. Flocculent suspensions were characterized based on floc diameter and density. Settling tests were performed in batch at different concentrations of the cellular suspensions and revealed high recovery of the solids in suspension in all cases. Flotation tests were accomplished using a mechanical agitated flotation cell and the process was favoured by the increase of the system agitation and for the presence of a cationic collector.<br>O trabalho aborda a recuperação de esporos da bactéria Bacillus sphaericus por floculação/sedimentação e flotação. O microrganismo foi produzido em batelada, utilizando-se meio de cultivo à base de leite desnatado, milhocina e sais minerais. Os melhores resultados de floculação foram obtidos com os floculantes CaCl2.2H2O, FeCl3.6H2O, Al2(SO4)3 e tanino, com concentrações ótimas de 1.500, 3.000, 2.000 e 1.700ppm, respectivamente. Os sistemas floculentos foram caracterizados através da determinação da densidade e do diâmetro médio dos flocos. Testes de sedimentação em batelada a diferentes concentrações das suspensões celulares revelaram elevados índices de recuperação dos sólidos em suspensão em todos os casos. Os ensaios de flotação foram realizados em célula de flotação mecânica, e o processo foi favorecido pelo aumento da agitação do sistema e pela presença de um coletor catiônico.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000400008Bacillus sphaericusflocculationsedimentationflotation |
| spellingShingle | Christine Lamenha Luna Carlos Edison Lopes Giulio Massarani Recovery of Bacillus sphaericus spores by flocculation/sedimentation and flotation Brazilian Archives of Biology and Technology Bacillus sphaericus flocculation sedimentation flotation |
| title | Recovery of Bacillus sphaericus spores by flocculation/sedimentation and flotation |
| title_full | Recovery of Bacillus sphaericus spores by flocculation/sedimentation and flotation |
| title_fullStr | Recovery of Bacillus sphaericus spores by flocculation/sedimentation and flotation |
| title_full_unstemmed | Recovery of Bacillus sphaericus spores by flocculation/sedimentation and flotation |
| title_short | Recovery of Bacillus sphaericus spores by flocculation/sedimentation and flotation |
| title_sort | recovery of bacillus sphaericus spores by flocculation sedimentation and flotation |
| topic | Bacillus sphaericus flocculation sedimentation flotation |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000400008 |
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