Sperm pretreatment with glutathione improves IVF embryos development through increasing the viability and antioxidative capacity of sex-sorted and unsorted bull semen
The antioxidant of reduced glutathione (GSH) is the most abundant thiol in cells for the maintenance of the intracellular redox balance. The study aimed to assay the effect of sperm treatment with GSH before incubation with oocytes on the development potential of embryos obtained by in vitro fertili...
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| Format: | Article |
| Language: | English |
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Elsevier
2016-10-01
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| Series: | Journal of Integrative Agriculture |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311916614028 |
| _version_ | 1818647676765339648 |
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| author | Ting-xi HU Hua-bin ZHU Wei-jun SUN Hai-sheng HAO Xue-ming ZHAO Wei-hua DU Zong-li WANG |
| author_facet | Ting-xi HU Hua-bin ZHU Wei-jun SUN Hai-sheng HAO Xue-ming ZHAO Wei-hua DU Zong-li WANG |
| author_sort | Ting-xi HU |
| collection | DOAJ |
| description | The antioxidant of reduced glutathione (GSH) is the most abundant thiol in cells for the maintenance of the intracellular redox balance. The study aimed to assay the effect of sperm treatment with GSH before incubation with oocytes on the development potential of embryos obtained by in vitro fertilization (IVF). Also the mitochondrial membrane potential (ΔΦm), plasma membrane integrity (viability), DNA fragmentation, reactive oxygen species (ROS) content, superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) activities, methane dicarboxylic aldehyde (MDA) level as indices of lipid peroxidation in sex-sorted and unsorted sperm from three bulls were investigated using flow cytometry and enzyme-labeled instrument individually. The results showed that 2 mmol L−1 GSH increased significantly the cleavage rate (86.68% vs. 82.78%), 4- to 8-cell rate (82.30% vs. 73.43%) and blastocyst rate (43.15% vs. 35.24%) of IVF embryos compared with untreated group. Furthermore, addition of GSH increased significantly the ΔΦm and viability, decreased the ratio of DNA fragmentation in sex-sorted or unsorted semen (P<0.05), except the sex-sorted semen from bull 019. Similarly, activities of SOD, CAT and GPx were increased significantly. However, the contents of MDA were decreased significantly both in sex-sorted and unsorted semen treated with GSH (P<0.05). These results suggest that sperm pretreatment with GSH during IVF can maintain better the viability and fertility of sperm through reducing apoptosis and increasing the antioxidant capacity, which improves the IVF embryos development. |
| first_indexed | 2024-12-17T01:06:19Z |
| format | Article |
| id | doaj.art-51a33444bb9f4292b818596a43274420 |
| institution | Directory Open Access Journal |
| issn | 2095-3119 |
| language | English |
| last_indexed | 2024-12-17T01:06:19Z |
| publishDate | 2016-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj.art-51a33444bb9f4292b818596a432744202022-12-21T22:09:14ZengElsevierJournal of Integrative Agriculture2095-31192016-10-01151023262335Sperm pretreatment with glutathione improves IVF embryos development through increasing the viability and antioxidative capacity of sex-sorted and unsorted bull semenTing-xi HU0Hua-bin ZHU1Wei-jun SUN2Hai-sheng HAO3Xue-ming ZHAO4Wei-hua DU5Zong-li WANG6College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, P.R. China; Embryo Biotechnology and Reproduction Laboratory, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R. China; HU Ting-xi, Mobile: +86-15300198828Embryo Biotechnology and Reproduction Laboratory, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R. ChinaEmbryo Biotechnology and Reproduction Laboratory, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R. ChinaEmbryo Biotechnology and Reproduction Laboratory, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R. ChinaEmbryo Biotechnology and Reproduction Laboratory, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R. ChinaEmbryo Biotechnology and Reproduction Laboratory, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R. China; DU Wei-hua, Tel: +86-10-62815892, Fax: +86-10-62815351College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, P.R. China; Correspondence WANG Zong-li, Tel/Fax: +86-931-7632148The antioxidant of reduced glutathione (GSH) is the most abundant thiol in cells for the maintenance of the intracellular redox balance. The study aimed to assay the effect of sperm treatment with GSH before incubation with oocytes on the development potential of embryos obtained by in vitro fertilization (IVF). Also the mitochondrial membrane potential (ΔΦm), plasma membrane integrity (viability), DNA fragmentation, reactive oxygen species (ROS) content, superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) activities, methane dicarboxylic aldehyde (MDA) level as indices of lipid peroxidation in sex-sorted and unsorted sperm from three bulls were investigated using flow cytometry and enzyme-labeled instrument individually. The results showed that 2 mmol L−1 GSH increased significantly the cleavage rate (86.68% vs. 82.78%), 4- to 8-cell rate (82.30% vs. 73.43%) and blastocyst rate (43.15% vs. 35.24%) of IVF embryos compared with untreated group. Furthermore, addition of GSH increased significantly the ΔΦm and viability, decreased the ratio of DNA fragmentation in sex-sorted or unsorted semen (P<0.05), except the sex-sorted semen from bull 019. Similarly, activities of SOD, CAT and GPx were increased significantly. However, the contents of MDA were decreased significantly both in sex-sorted and unsorted semen treated with GSH (P<0.05). These results suggest that sperm pretreatment with GSH during IVF can maintain better the viability and fertility of sperm through reducing apoptosis and increasing the antioxidant capacity, which improves the IVF embryos development.http://www.sciencedirect.com/science/article/pii/S2095311916614028GSHapoptosisantioxidant enzymesunsorted semensex-sorted semen |
| spellingShingle | Ting-xi HU Hua-bin ZHU Wei-jun SUN Hai-sheng HAO Xue-ming ZHAO Wei-hua DU Zong-li WANG Sperm pretreatment with glutathione improves IVF embryos development through increasing the viability and antioxidative capacity of sex-sorted and unsorted bull semen Journal of Integrative Agriculture GSH apoptosis antioxidant enzymes unsorted semen sex-sorted semen |
| title | Sperm pretreatment with glutathione improves IVF embryos development through increasing the viability and antioxidative capacity of sex-sorted and unsorted bull semen |
| title_full | Sperm pretreatment with glutathione improves IVF embryos development through increasing the viability and antioxidative capacity of sex-sorted and unsorted bull semen |
| title_fullStr | Sperm pretreatment with glutathione improves IVF embryos development through increasing the viability and antioxidative capacity of sex-sorted and unsorted bull semen |
| title_full_unstemmed | Sperm pretreatment with glutathione improves IVF embryos development through increasing the viability and antioxidative capacity of sex-sorted and unsorted bull semen |
| title_short | Sperm pretreatment with glutathione improves IVF embryos development through increasing the viability and antioxidative capacity of sex-sorted and unsorted bull semen |
| title_sort | sperm pretreatment with glutathione improves ivf embryos development through increasing the viability and antioxidative capacity of sex sorted and unsorted bull semen |
| topic | GSH apoptosis antioxidant enzymes unsorted semen sex-sorted semen |
| url | http://www.sciencedirect.com/science/article/pii/S2095311916614028 |
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