Transcriptomic Analysis Reveals Genes Associated with the Regulation of Peach Fruit Softening and Senescence during Storage
Peach (<i>Prunus persica</i> (L.) Batsch) is a highly desirable fruit that is consumed around the world. However, the peach fruit is highly perishable after harvest, a characteristic that limits the distribution and supply to the market and causes heavy economic losses. Thus, peach fruit...
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| Format: | Article |
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MDPI AG
2023-04-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/12/8/1648 |
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| author | Shaolei Guo Ruijuan Ma Jianlan Xu Binbin Zhang Mingliang Yu Zhihong Gao |
| author_facet | Shaolei Guo Ruijuan Ma Jianlan Xu Binbin Zhang Mingliang Yu Zhihong Gao |
| author_sort | Shaolei Guo |
| collection | DOAJ |
| description | Peach (<i>Prunus persica</i> (L.) Batsch) is a highly desirable fruit that is consumed around the world. However, the peach fruit is highly perishable after harvest, a characteristic that limits the distribution and supply to the market and causes heavy economic losses. Thus, peach fruit softening and senescence after harvest urgently need to be addressed. In the current study, transcriptomic analysis was performed to identify candidate genes associated with peach fruit softening and senescence, comparing peach fruit from cultivars with different flesh textures, namely melting and stony hard (SH) flesh textures during storage at room temperature. The mitogen-activated protein kinase signaling pathway-plant and plant hormone signal transduction pathways were associated with peach fruit softening and senescence according to the Venn diagram analysis and weighted gene co-expression network analysis. The expression levels of seven genes, including <i>Prupe.1G034300</i>, <i>Prupe.2G176900</i>, <i>Prupe.3G024700</i>, <i>Prupe.3G098100</i>, <i>Prupe.6G226100</i>, <i>Prupe.7G234800,</i> and <i>Prupe.7G247500</i>, were higher in melting peach fruit than in SH peach fruit during storage. Furthermore, the SH peach fruit softened rapidly after 1-naphthylacetic acid treatment, during which the levels of expression of these seven genes, determined by a quantitative reverse transcription polymerase chain reaction, were strongly induced and upregulated. Thus, these seven genes may play essential roles in regulating peach fruit softening and senescence. |
| first_indexed | 2024-03-11T05:01:03Z |
| format | Article |
| id | doaj.art-51a59a85182845d88995bd45dd51765d |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-11T05:01:03Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-51a59a85182845d88995bd45dd51765d2023-11-17T19:14:41ZengMDPI AGFoods2304-81582023-04-01128164810.3390/foods12081648Transcriptomic Analysis Reveals Genes Associated with the Regulation of Peach Fruit Softening and Senescence during StorageShaolei Guo0Ruijuan Ma1Jianlan Xu2Binbin Zhang3Mingliang Yu4Zhihong Gao5Institute of Pomology, Jiangsu Academy of Agricultural Sciences/Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Nanjing 210014, ChinaInstitute of Pomology, Jiangsu Academy of Agricultural Sciences/Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Nanjing 210014, ChinaInstitute of Pomology, Jiangsu Academy of Agricultural Sciences/Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Nanjing 210014, ChinaInstitute of Pomology, Jiangsu Academy of Agricultural Sciences/Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Nanjing 210014, ChinaInstitute of Pomology, Jiangsu Academy of Agricultural Sciences/Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Nanjing 210014, ChinaCollege of Horticulture, Nanjing Agricultural University, Nanjing 210095, ChinaPeach (<i>Prunus persica</i> (L.) Batsch) is a highly desirable fruit that is consumed around the world. However, the peach fruit is highly perishable after harvest, a characteristic that limits the distribution and supply to the market and causes heavy economic losses. Thus, peach fruit softening and senescence after harvest urgently need to be addressed. In the current study, transcriptomic analysis was performed to identify candidate genes associated with peach fruit softening and senescence, comparing peach fruit from cultivars with different flesh textures, namely melting and stony hard (SH) flesh textures during storage at room temperature. The mitogen-activated protein kinase signaling pathway-plant and plant hormone signal transduction pathways were associated with peach fruit softening and senescence according to the Venn diagram analysis and weighted gene co-expression network analysis. The expression levels of seven genes, including <i>Prupe.1G034300</i>, <i>Prupe.2G176900</i>, <i>Prupe.3G024700</i>, <i>Prupe.3G098100</i>, <i>Prupe.6G226100</i>, <i>Prupe.7G234800,</i> and <i>Prupe.7G247500</i>, were higher in melting peach fruit than in SH peach fruit during storage. Furthermore, the SH peach fruit softened rapidly after 1-naphthylacetic acid treatment, during which the levels of expression of these seven genes, determined by a quantitative reverse transcription polymerase chain reaction, were strongly induced and upregulated. Thus, these seven genes may play essential roles in regulating peach fruit softening and senescence.https://www.mdpi.com/2304-8158/12/8/1648auxinpeachqRT-PCRsofteningtranscriptomic analysis |
| spellingShingle | Shaolei Guo Ruijuan Ma Jianlan Xu Binbin Zhang Mingliang Yu Zhihong Gao Transcriptomic Analysis Reveals Genes Associated with the Regulation of Peach Fruit Softening and Senescence during Storage Foods auxin peach qRT-PCR softening transcriptomic analysis |
| title | Transcriptomic Analysis Reveals Genes Associated with the Regulation of Peach Fruit Softening and Senescence during Storage |
| title_full | Transcriptomic Analysis Reveals Genes Associated with the Regulation of Peach Fruit Softening and Senescence during Storage |
| title_fullStr | Transcriptomic Analysis Reveals Genes Associated with the Regulation of Peach Fruit Softening and Senescence during Storage |
| title_full_unstemmed | Transcriptomic Analysis Reveals Genes Associated with the Regulation of Peach Fruit Softening and Senescence during Storage |
| title_short | Transcriptomic Analysis Reveals Genes Associated with the Regulation of Peach Fruit Softening and Senescence during Storage |
| title_sort | transcriptomic analysis reveals genes associated with the regulation of peach fruit softening and senescence during storage |
| topic | auxin peach qRT-PCR softening transcriptomic analysis |
| url | https://www.mdpi.com/2304-8158/12/8/1648 |
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