MicroRNA-based promotion of human neuronal differentiation and subtype specification.
MicroRNAs are key regulators of neural cell proliferation, differentiation and fate choice. Due to the limited access to human primary neural tissue, the role of microRNAs in human neuronal differentiation remains largely unknown. Here, we use a population of long-term self-renewing neuroepithelial-...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3601127?pdf=render |
| _version_ | 1828784241406640128 |
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| author | Laura Stappert Lodovica Borghese Beate Roese-Koerner Sandra Weinhold Philipp Koch Stefanie Terstegge Markus Uhrberg Peter Wernet Oliver Brüstle |
| author_facet | Laura Stappert Lodovica Borghese Beate Roese-Koerner Sandra Weinhold Philipp Koch Stefanie Terstegge Markus Uhrberg Peter Wernet Oliver Brüstle |
| author_sort | Laura Stappert |
| collection | DOAJ |
| description | MicroRNAs are key regulators of neural cell proliferation, differentiation and fate choice. Due to the limited access to human primary neural tissue, the role of microRNAs in human neuronal differentiation remains largely unknown. Here, we use a population of long-term self-renewing neuroepithelial-like stem cells (lt-NES cells) derived from human embryonic stem cells to study the expression and function of microRNAs at early stages of human neural stem cell differentiation and neuronal lineage decision. Based on microRNA expression profiling followed by gain- and loss-of-function analyses in lt-NES cells and their neuronal progeny, we demonstrate that miR-153, miR-324-5p/3p and miR-181a/a contribute to the shift of lt-NES cells from self-renewal to neuronal differentiation. We further show that miR-125b and miR-181a specifically promote the generation of neurons of dopaminergic fate, whereas miR-181a inhibits the development of this neurotransmitter subtype. Our data demonstrate that time-controlled modulation of specific microRNA activities not only regulates human neural stem cell self-renewal and differentiation but also contributes to the development of defined neuronal subtypes. |
| first_indexed | 2024-12-11T23:27:58Z |
| format | Article |
| id | doaj.art-51a637531df24406af0f692eaafe87f7 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-11T23:27:58Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-51a637531df24406af0f692eaafe87f72022-12-22T00:46:08ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0183e5901110.1371/journal.pone.0059011MicroRNA-based promotion of human neuronal differentiation and subtype specification.Laura StappertLodovica BorgheseBeate Roese-KoernerSandra WeinholdPhilipp KochStefanie TersteggeMarkus UhrbergPeter WernetOliver BrüstleMicroRNAs are key regulators of neural cell proliferation, differentiation and fate choice. Due to the limited access to human primary neural tissue, the role of microRNAs in human neuronal differentiation remains largely unknown. Here, we use a population of long-term self-renewing neuroepithelial-like stem cells (lt-NES cells) derived from human embryonic stem cells to study the expression and function of microRNAs at early stages of human neural stem cell differentiation and neuronal lineage decision. Based on microRNA expression profiling followed by gain- and loss-of-function analyses in lt-NES cells and their neuronal progeny, we demonstrate that miR-153, miR-324-5p/3p and miR-181a/a contribute to the shift of lt-NES cells from self-renewal to neuronal differentiation. We further show that miR-125b and miR-181a specifically promote the generation of neurons of dopaminergic fate, whereas miR-181a inhibits the development of this neurotransmitter subtype. Our data demonstrate that time-controlled modulation of specific microRNA activities not only regulates human neural stem cell self-renewal and differentiation but also contributes to the development of defined neuronal subtypes.http://europepmc.org/articles/PMC3601127?pdf=render |
| spellingShingle | Laura Stappert Lodovica Borghese Beate Roese-Koerner Sandra Weinhold Philipp Koch Stefanie Terstegge Markus Uhrberg Peter Wernet Oliver Brüstle MicroRNA-based promotion of human neuronal differentiation and subtype specification. PLoS ONE |
| title | MicroRNA-based promotion of human neuronal differentiation and subtype specification. |
| title_full | MicroRNA-based promotion of human neuronal differentiation and subtype specification. |
| title_fullStr | MicroRNA-based promotion of human neuronal differentiation and subtype specification. |
| title_full_unstemmed | MicroRNA-based promotion of human neuronal differentiation and subtype specification. |
| title_short | MicroRNA-based promotion of human neuronal differentiation and subtype specification. |
| title_sort | microrna based promotion of human neuronal differentiation and subtype specification |
| url | http://europepmc.org/articles/PMC3601127?pdf=render |
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