RNA-Based Technologies for Engineering Plant Virus Resistance
In recent years, non-coding RNAs (ncRNAs) have gained unprecedented attention as new and crucial players in the regulation of numerous cellular processes and disease responses. In this review, we describe how diverse ncRNAs, including both small RNAs and long ncRNAs, may be used to engineer resistan...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-01-01
|
| Series: | Plants |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2223-7747/10/1/82 |
| _version_ | 1797542528086441984 |
|---|---|
| author | Michael Taliansky Viktoria Samarskaya Sergey K. Zavriev Igor Fesenko Natalia O. Kalinina Andrew J. Love |
| author_facet | Michael Taliansky Viktoria Samarskaya Sergey K. Zavriev Igor Fesenko Natalia O. Kalinina Andrew J. Love |
| author_sort | Michael Taliansky |
| collection | DOAJ |
| description | In recent years, non-coding RNAs (ncRNAs) have gained unprecedented attention as new and crucial players in the regulation of numerous cellular processes and disease responses. In this review, we describe how diverse ncRNAs, including both small RNAs and long ncRNAs, may be used to engineer resistance against plant viruses. We discuss how double-stranded RNAs and small RNAs, such as artificial microRNAs and trans-acting small interfering RNAs, either produced in transgenic plants or delivered exogenously to non-transgenic plants, may constitute powerful RNA interference (RNAi)-based technology that can be exploited to control plant viruses. Additionally, we describe how RNA guided CRISPR-CAS gene-editing systems have been deployed to inhibit plant virus infections, and we provide a comparative analysis of RNAi approaches and CRISPR-Cas technology. The two main strategies for engineering virus resistance are also discussed, including direct targeting of viral DNA or RNA, or inactivation of plant host susceptibility genes. We also elaborate on the challenges that need to be overcome before such technologies can be broadly exploited for crop protection against viruses. |
| first_indexed | 2024-03-10T13:31:50Z |
| format | Article |
| id | doaj.art-51a9716fc8aa4eae86fb3ce8200e3b18 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T13:31:50Z |
| publishDate | 2021-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-51a9716fc8aa4eae86fb3ce8200e3b182023-11-21T07:50:21ZengMDPI AGPlants2223-77472021-01-011018210.3390/plants10010082RNA-Based Technologies for Engineering Plant Virus ResistanceMichael Taliansky0Viktoria Samarskaya1Sergey K. Zavriev2Igor Fesenko3Natalia O. Kalinina4Andrew J. Love5Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, 117997 Moscow, RussiaShemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, 117997 Moscow, RussiaShemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, 117997 Moscow, RussiaShemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, 117997 Moscow, RussiaShemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, 117997 Moscow, RussiaThe James Hutton Institute, Invergowrie, Dundee DD2 5DA, UKIn recent years, non-coding RNAs (ncRNAs) have gained unprecedented attention as new and crucial players in the regulation of numerous cellular processes and disease responses. In this review, we describe how diverse ncRNAs, including both small RNAs and long ncRNAs, may be used to engineer resistance against plant viruses. We discuss how double-stranded RNAs and small RNAs, such as artificial microRNAs and trans-acting small interfering RNAs, either produced in transgenic plants or delivered exogenously to non-transgenic plants, may constitute powerful RNA interference (RNAi)-based technology that can be exploited to control plant viruses. Additionally, we describe how RNA guided CRISPR-CAS gene-editing systems have been deployed to inhibit plant virus infections, and we provide a comparative analysis of RNAi approaches and CRISPR-Cas technology. The two main strategies for engineering virus resistance are also discussed, including direct targeting of viral DNA or RNA, or inactivation of plant host susceptibility genes. We also elaborate on the challenges that need to be overcome before such technologies can be broadly exploited for crop protection against viruses.https://www.mdpi.com/2223-7747/10/1/82dsRNAsiRNAmiRNAlncRNAamiRNAtasiRNA |
| spellingShingle | Michael Taliansky Viktoria Samarskaya Sergey K. Zavriev Igor Fesenko Natalia O. Kalinina Andrew J. Love RNA-Based Technologies for Engineering Plant Virus Resistance Plants dsRNA siRNA miRNA lncRNA amiRNA tasiRNA |
| title | RNA-Based Technologies for Engineering Plant Virus Resistance |
| title_full | RNA-Based Technologies for Engineering Plant Virus Resistance |
| title_fullStr | RNA-Based Technologies for Engineering Plant Virus Resistance |
| title_full_unstemmed | RNA-Based Technologies for Engineering Plant Virus Resistance |
| title_short | RNA-Based Technologies for Engineering Plant Virus Resistance |
| title_sort | rna based technologies for engineering plant virus resistance |
| topic | dsRNA siRNA miRNA lncRNA amiRNA tasiRNA |
| url | https://www.mdpi.com/2223-7747/10/1/82 |
| work_keys_str_mv | AT michaeltaliansky rnabasedtechnologiesforengineeringplantvirusresistance AT viktoriasamarskaya rnabasedtechnologiesforengineeringplantvirusresistance AT sergeykzavriev rnabasedtechnologiesforengineeringplantvirusresistance AT igorfesenko rnabasedtechnologiesforengineeringplantvirusresistance AT nataliaokalinina rnabasedtechnologiesforengineeringplantvirusresistance AT andrewjlove rnabasedtechnologiesforengineeringplantvirusresistance |