Mapping of IgE-binding regions on recombinant Cyn d 1, a major allergen from Bermuda Grass Pollen (BGP)
<p>Abstract</p> <p>Background</p> <p>Bermuda grass (<it>Cynodon dactylon</it>; subfamily Chloridoideae) is an important source of seasonal aeroallergens in warm tropical and sub-tropical areas worldwide. Improved approaches to diagnosis and therapy of allerg...
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| Format: | Article |
| Language: | English |
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BMC
2009-02-01
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| Series: | Clinical and Molecular Allergy |
| Online Access: | http://www.clinicalmolecularallergy.com/content/7/1/3 |
| _version_ | 1818145845962342400 |
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| author | Bhalla Prem L Tiwari Ruby Singh Mohan B |
| author_facet | Bhalla Prem L Tiwari Ruby Singh Mohan B |
| author_sort | Bhalla Prem L |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Bermuda grass (<it>Cynodon dactylon</it>; subfamily Chloridoideae) is an important source of seasonal aeroallergens in warm tropical and sub-tropical areas worldwide. Improved approaches to diagnosis and therapy of allergic diseases require a thorough understanding of the structure and epitopes on the allergen molecule that are crucial for the antigen-antibody interaction. This study describes the localization of the human IgE-binding regions of the major group 1 pollen allergen Cyn d 1 from Bermuda grass.</p> <p>Methods</p> <p>A cDNA library was constructed from Bermuda grass pollen (BGP) using a Lambda gt11 expression vector. The gene encoding the Cyn d 1 allergen was isolated by screening the library with a mouse monoclonal antibody raised against grass group 1 allergen. In order to characterize the IgE epitopes on Cyn d 1, seven overlapping fragments and three deletion mutants were cloned and over-expressed in E. coli. The recombinant fragments and deletion mutants were evaluated for their comparative IgE reactivity with sera of non atopic individuals and grass pollen allergic patients by ELISA and a dot-blot assay.</p> <p>Results</p> <p>Analysis of IgE binding regions by overlapping fragments and deletion mutants identified two major allergenic regions corresponding to amino acids 120–170 and 224–244. Deletion of either or both regions led to a significant reduction in IgE binding, emphasizing the importance of the C-terminal region on Cyn d 1 in epitope-IgE interaction.</p> <p>Conclusion</p> <p>Anti-Cyn d 1 IgE antibodies from allergic human sera recognize two epitopes located at the C-terminal end of the molecule. These data will enable the design of improved diagnostic and therapeutic approaches for BGP hypersensitivity.</p> |
| first_indexed | 2024-12-11T12:09:56Z |
| format | Article |
| id | doaj.art-51ab82d6b39b41858d9d2b6e76321651 |
| institution | Directory Open Access Journal |
| issn | 1476-7961 |
| language | English |
| last_indexed | 2024-12-11T12:09:56Z |
| publishDate | 2009-02-01 |
| publisher | BMC |
| record_format | Article |
| series | Clinical and Molecular Allergy |
| spelling | doaj.art-51ab82d6b39b41858d9d2b6e763216512022-12-22T01:07:50ZengBMCClinical and Molecular Allergy1476-79612009-02-0171310.1186/1476-7961-7-3Mapping of IgE-binding regions on recombinant Cyn d 1, a major allergen from Bermuda Grass Pollen (BGP)Bhalla Prem LTiwari RubySingh Mohan B<p>Abstract</p> <p>Background</p> <p>Bermuda grass (<it>Cynodon dactylon</it>; subfamily Chloridoideae) is an important source of seasonal aeroallergens in warm tropical and sub-tropical areas worldwide. Improved approaches to diagnosis and therapy of allergic diseases require a thorough understanding of the structure and epitopes on the allergen molecule that are crucial for the antigen-antibody interaction. This study describes the localization of the human IgE-binding regions of the major group 1 pollen allergen Cyn d 1 from Bermuda grass.</p> <p>Methods</p> <p>A cDNA library was constructed from Bermuda grass pollen (BGP) using a Lambda gt11 expression vector. The gene encoding the Cyn d 1 allergen was isolated by screening the library with a mouse monoclonal antibody raised against grass group 1 allergen. In order to characterize the IgE epitopes on Cyn d 1, seven overlapping fragments and three deletion mutants were cloned and over-expressed in E. coli. The recombinant fragments and deletion mutants were evaluated for their comparative IgE reactivity with sera of non atopic individuals and grass pollen allergic patients by ELISA and a dot-blot assay.</p> <p>Results</p> <p>Analysis of IgE binding regions by overlapping fragments and deletion mutants identified two major allergenic regions corresponding to amino acids 120–170 and 224–244. Deletion of either or both regions led to a significant reduction in IgE binding, emphasizing the importance of the C-terminal region on Cyn d 1 in epitope-IgE interaction.</p> <p>Conclusion</p> <p>Anti-Cyn d 1 IgE antibodies from allergic human sera recognize two epitopes located at the C-terminal end of the molecule. These data will enable the design of improved diagnostic and therapeutic approaches for BGP hypersensitivity.</p>http://www.clinicalmolecularallergy.com/content/7/1/3 |
| spellingShingle | Bhalla Prem L Tiwari Ruby Singh Mohan B Mapping of IgE-binding regions on recombinant Cyn d 1, a major allergen from Bermuda Grass Pollen (BGP) Clinical and Molecular Allergy |
| title | Mapping of IgE-binding regions on recombinant Cyn d 1, a major allergen from Bermuda Grass Pollen (BGP) |
| title_full | Mapping of IgE-binding regions on recombinant Cyn d 1, a major allergen from Bermuda Grass Pollen (BGP) |
| title_fullStr | Mapping of IgE-binding regions on recombinant Cyn d 1, a major allergen from Bermuda Grass Pollen (BGP) |
| title_full_unstemmed | Mapping of IgE-binding regions on recombinant Cyn d 1, a major allergen from Bermuda Grass Pollen (BGP) |
| title_short | Mapping of IgE-binding regions on recombinant Cyn d 1, a major allergen from Bermuda Grass Pollen (BGP) |
| title_sort | mapping of ige binding regions on recombinant cyn d 1 a major allergen from bermuda grass pollen bgp |
| url | http://www.clinicalmolecularallergy.com/content/7/1/3 |
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