Icariin ameliorates lung injury in severe acute pancreatitis of rat models
Objective To evaluate the protective effect and inflammation inhibition mechanism of icariin (ICA) on lung injury in rat model with severe acute pancreatitis (SAP). Methods The rats were randomly divided into sham operation group (sham group), model group (SAP group, 5% sodium taurocholate was retro...
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| Format: | Article |
| Language: | zho |
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Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.
2022-09-01
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| Series: | Jichu yixue yu linchuang |
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| Online Access: | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-42-9-1400.pdf |
| _version_ | 1797365848447385600 |
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| author | GU Wen-hao, GUO Fei-xia,XU Yu-peng, LU Xin-yu, HU Qing-qing |
| author_facet | GU Wen-hao, GUO Fei-xia,XU Yu-peng, LU Xin-yu, HU Qing-qing |
| author_sort | GU Wen-hao, GUO Fei-xia,XU Yu-peng, LU Xin-yu, HU Qing-qing |
| collection | DOAJ |
| description | Objective To evaluate the protective effect and inflammation inhibition mechanism of icariin (ICA) on lung injury in rat model with severe acute pancreatitis (SAP). Methods The rats were randomly divided into sham operation group (sham group), model group (SAP group, 5% sodium taurocholate was retrograde pumped into bile duct according to 0.1 mL/kg through micropump) and experimental group (ICA group, icariin 80 mg/kg intervention was given 2 hours before modeling). There were 6 rats in each group. Twenty-four hours after the establishment of the model, the inferior vena cava blood, pancreatic tissue and lung tissue were collected. The pathological injury of pancreas and lung was observed by HE staining; The activity of amylase, serum IL-6, IL-1β and TNF-α were measured by ELISA; The expression of inflammatory factors IL-6, IL-1β and TNF-α in lung tissue was determined by immunohistochemical staining; And the ratio of phosphorylated JNK/JNK to phosphorylated NF-κB/NF-κB in lung tissue was measured by Western blot. Results The pathological scores of pancreas and lung in SAP group were significantly higher than those in sham group; And the pathological scores of pancreas and lung in ICA group were significantly lower than those in SAP group. Serum amylase activity, serum IL-6, IL-1β and TNF-α content and the expression of IL-6, IL-1β and TNF-α in lung tissue in SAP group were significantly higher than those in sham group; While serum amylase activity, serum IL-6, IL-1β and TNF-α content and the expression of IL-6, IL-1β and TNF-α in lung tissue in ICA group were significantly lower than those in SAP group. The level of phosphorylated JNK and phosphorylated NF-κB in lung tissue of SAP group were significantly higher than those of sham group; While icariin might significantly reduce the level of phosphorylated JNK and phosphorylated NF-κB in lung tissue. Conclusions ICA ameliorates lung injury in SAP rat model by inhibiting acute inflammation response,which is potentially mediated by JNK/NF-κB signal pathway. |
| first_indexed | 2024-03-08T16:54:51Z |
| format | Article |
| id | doaj.art-51abbc535d2e471da80cf63027e3e675 |
| institution | Directory Open Access Journal |
| issn | 1001-6325 |
| language | zho |
| last_indexed | 2024-03-08T16:54:51Z |
| publishDate | 2022-09-01 |
| publisher | Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College. |
| record_format | Article |
| series | Jichu yixue yu linchuang |
| spelling | doaj.art-51abbc535d2e471da80cf63027e3e6752024-01-05T02:36:18ZzhoInstitute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.Jichu yixue yu linchuang1001-63252022-09-014291400140510.16352/j.issn.1001-6325.2022.09.1400Icariin ameliorates lung injury in severe acute pancreatitis of rat modelsGU Wen-hao, GUO Fei-xia,XU Yu-peng, LU Xin-yu, HU Qing-qing01. Zhejiang University of Traditional Chinese Medicine, Hangzhou 310000;;2. Department of Internal Medicine, Wenzhou Lucheng District People's Hospital, Wenzhou 325000;;3. Laboratory of Translational Medicine;;4. the First Clinical Medical College;;5. Department of Pediatrics, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, ChinaObjective To evaluate the protective effect and inflammation inhibition mechanism of icariin (ICA) on lung injury in rat model with severe acute pancreatitis (SAP). Methods The rats were randomly divided into sham operation group (sham group), model group (SAP group, 5% sodium taurocholate was retrograde pumped into bile duct according to 0.1 mL/kg through micropump) and experimental group (ICA group, icariin 80 mg/kg intervention was given 2 hours before modeling). There were 6 rats in each group. Twenty-four hours after the establishment of the model, the inferior vena cava blood, pancreatic tissue and lung tissue were collected. The pathological injury of pancreas and lung was observed by HE staining; The activity of amylase, serum IL-6, IL-1β and TNF-α were measured by ELISA; The expression of inflammatory factors IL-6, IL-1β and TNF-α in lung tissue was determined by immunohistochemical staining; And the ratio of phosphorylated JNK/JNK to phosphorylated NF-κB/NF-κB in lung tissue was measured by Western blot. Results The pathological scores of pancreas and lung in SAP group were significantly higher than those in sham group; And the pathological scores of pancreas and lung in ICA group were significantly lower than those in SAP group. Serum amylase activity, serum IL-6, IL-1β and TNF-α content and the expression of IL-6, IL-1β and TNF-α in lung tissue in SAP group were significantly higher than those in sham group; While serum amylase activity, serum IL-6, IL-1β and TNF-α content and the expression of IL-6, IL-1β and TNF-α in lung tissue in ICA group were significantly lower than those in SAP group. The level of phosphorylated JNK and phosphorylated NF-κB in lung tissue of SAP group were significantly higher than those of sham group; While icariin might significantly reduce the level of phosphorylated JNK and phosphorylated NF-κB in lung tissue. Conclusions ICA ameliorates lung injury in SAP rat model by inhibiting acute inflammation response,which is potentially mediated by JNK/NF-κB signal pathway.http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-42-9-1400.pdficariin| inflammation| c-jun n-terminal kinase(jnk)| nf-κb| severe acute pancreatitis| lung injury |
| spellingShingle | GU Wen-hao, GUO Fei-xia,XU Yu-peng, LU Xin-yu, HU Qing-qing Icariin ameliorates lung injury in severe acute pancreatitis of rat models Jichu yixue yu linchuang icariin| inflammation| c-jun n-terminal kinase(jnk)| nf-κb| severe acute pancreatitis| lung injury |
| title | Icariin ameliorates lung injury in severe acute pancreatitis of rat models |
| title_full | Icariin ameliorates lung injury in severe acute pancreatitis of rat models |
| title_fullStr | Icariin ameliorates lung injury in severe acute pancreatitis of rat models |
| title_full_unstemmed | Icariin ameliorates lung injury in severe acute pancreatitis of rat models |
| title_short | Icariin ameliorates lung injury in severe acute pancreatitis of rat models |
| title_sort | icariin ameliorates lung injury in severe acute pancreatitis of rat models |
| topic | icariin| inflammation| c-jun n-terminal kinase(jnk)| nf-κb| severe acute pancreatitis| lung injury |
| url | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-42-9-1400.pdf |
| work_keys_str_mv | AT guwenhaoguofeixiaxuyupengluxinyuhuqingqing icariinameliorateslunginjuryinsevereacutepancreatitisofratmodels |