Changes in serum cytokine profile and deficit severity in patients with relapsing-remitting multiple sclerosis
Introduction: In experimental autoimmune encephalomyelitis, neurological deficit correlates with axonal loss and the CD8+ T cells are a likely mediator of axonal damage. In relapsing-remitting multiple sclerosis, there is a correlation of the immune inflammatory activity in the lesion foci with the...
| Main Author: | |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Pensoft Publishers
2023-08-01
|
| Series: | Folia Medica |
| Online Access: | https://foliamedica.bg/article/86576/download/pdf/ |
| _version_ | 1797726080150274048 |
|---|---|
| author | Georgi Slavov |
| author_facet | Georgi Slavov |
| author_sort | Georgi Slavov |
| collection | DOAJ |
| description | Introduction: In experimental autoimmune encephalomyelitis, neurological deficit correlates with axonal loss and the CD8+ T cells are a likely mediator of axonal damage. In relapsing-remitting multiple sclerosis, there is a correlation of the immune inflammatory activity in the lesion foci with the axon transection. Aim: To evaluate the changes occurring in the serum concentrations of TNF-α, IFN-γ, IL17, TGF-β1, IL4, and IL10 during relapse and remission, and their correlations with the degree of neurological deficit. Materials and methods: In an open, prospective, case-control study conducted between 2012 and 2014, we examined 86 people: 46 patients (33 women and 13 men) and 40 healthy individuals (20 women and 20 men). Serum cytokine concentrations were analyzed using ELISA – once in the controls, twice in the patients during the relapse and remission of the condition. The collected data were analyzed using SPSS 19.0. We used the Kolmogorov-Smirnov test, the independent sample t-test, the Spearman and Pearson correlation, the Mann-Whitney test, and regression analysis. Results: Immune imbalance was found in the patients compared to the healthy controls in both relapse and remission. During the relapse, the IFN-γ levels were significantly increased compared to the levels in remission (p=0.017). During remission, the deficit was statistically significantly improved (p<0.001) and the anti-inflammatory IL4 and TGF-β1 were increased compared to their levels in the exacerbation period (p=0.006 and p=0.009, respectively). There was a causal relationship between the serum concentrations of TNF-α and EDSS in relapse (Vanetto-significance). During this phase, the regression analysis established two factors that had statistically significant influence on the deficit severity – TNF-α and IL17 (t=2.093, p=0.042; t=−2.140, p=0.038). Conclusions: IL17 and TNF-α serum concentrations are significant factors for the neurological deficit severity. The levels of IFN-γ, IL4, and TGF-β1 during both periods are criteria for evaluation of the immune inflammatory activity. |
| first_indexed | 2024-03-12T10:40:39Z |
| format | Article |
| id | doaj.art-51abc019e1dc403ca319cec22b731abf |
| institution | Directory Open Access Journal |
| issn | 1314-2143 |
| language | English |
| last_indexed | 2024-03-12T10:40:39Z |
| publishDate | 2023-08-01 |
| publisher | Pensoft Publishers |
| record_format | Article |
| series | Folia Medica |
| spelling | doaj.art-51abc019e1dc403ca319cec22b731abf2023-09-02T08:11:14ZengPensoft PublishersFolia Medica1314-21432023-08-0165462563010.3897/folmed.65.e8657686576Changes in serum cytokine profile and deficit severity in patients with relapsing-remitting multiple sclerosisGeorgi Slavov0Medical University of PlovdivIntroduction: In experimental autoimmune encephalomyelitis, neurological deficit correlates with axonal loss and the CD8+ T cells are a likely mediator of axonal damage. In relapsing-remitting multiple sclerosis, there is a correlation of the immune inflammatory activity in the lesion foci with the axon transection. Aim: To evaluate the changes occurring in the serum concentrations of TNF-α, IFN-γ, IL17, TGF-β1, IL4, and IL10 during relapse and remission, and their correlations with the degree of neurological deficit. Materials and methods: In an open, prospective, case-control study conducted between 2012 and 2014, we examined 86 people: 46 patients (33 women and 13 men) and 40 healthy individuals (20 women and 20 men). Serum cytokine concentrations were analyzed using ELISA – once in the controls, twice in the patients during the relapse and remission of the condition. The collected data were analyzed using SPSS 19.0. We used the Kolmogorov-Smirnov test, the independent sample t-test, the Spearman and Pearson correlation, the Mann-Whitney test, and regression analysis. Results: Immune imbalance was found in the patients compared to the healthy controls in both relapse and remission. During the relapse, the IFN-γ levels were significantly increased compared to the levels in remission (p=0.017). During remission, the deficit was statistically significantly improved (p<0.001) and the anti-inflammatory IL4 and TGF-β1 were increased compared to their levels in the exacerbation period (p=0.006 and p=0.009, respectively). There was a causal relationship between the serum concentrations of TNF-α and EDSS in relapse (Vanetto-significance). During this phase, the regression analysis established two factors that had statistically significant influence on the deficit severity – TNF-α and IL17 (t=2.093, p=0.042; t=−2.140, p=0.038). Conclusions: IL17 and TNF-α serum concentrations are significant factors for the neurological deficit severity. The levels of IFN-γ, IL4, and TGF-β1 during both periods are criteria for evaluation of the immune inflammatory activity.https://foliamedica.bg/article/86576/download/pdf/ |
| spellingShingle | Georgi Slavov Changes in serum cytokine profile and deficit severity in patients with relapsing-remitting multiple sclerosis Folia Medica |
| title | Changes in serum cytokine profile and deficit severity in patients with relapsing-remitting multiple sclerosis |
| title_full | Changes in serum cytokine profile and deficit severity in patients with relapsing-remitting multiple sclerosis |
| title_fullStr | Changes in serum cytokine profile and deficit severity in patients with relapsing-remitting multiple sclerosis |
| title_full_unstemmed | Changes in serum cytokine profile and deficit severity in patients with relapsing-remitting multiple sclerosis |
| title_short | Changes in serum cytokine profile and deficit severity in patients with relapsing-remitting multiple sclerosis |
| title_sort | changes in serum cytokine profile and deficit severity in patients with relapsing remitting multiple sclerosis |
| url | https://foliamedica.bg/article/86576/download/pdf/ |
| work_keys_str_mv | AT georgislavov changesinserumcytokineprofileanddeficitseverityinpatientswithrelapsingremittingmultiplesclerosis |