Effects of Hochuekkito, a traditional Japanese medicine (Kampo), on reproduction of aging female mice
Abstract Purpose To determine the potentials of Hochuekkito (HET) treatment for aging infertility. Methods Mice at 36 weeks of age were fed without (control, n = 40) or with low (100 mg/kg/day, n = 24) and high (1000 mg/kg/day, n = 38) doses of HET for 12 weeks. Aging animals at 48 weeks of age were...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2022-01-01
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| Series: | Reproductive Medicine and Biology |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/rmb2.12425 |
| _version_ | 1797976003295838208 |
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| author | Kim Cat Tuyen Vo Yorino Sato Yuta Kawagoe Kazuhiro Kawamura |
| author_facet | Kim Cat Tuyen Vo Yorino Sato Yuta Kawagoe Kazuhiro Kawamura |
| author_sort | Kim Cat Tuyen Vo |
| collection | DOAJ |
| description | Abstract Purpose To determine the potentials of Hochuekkito (HET) treatment for aging infertility. Methods Mice at 36 weeks of age were fed without (control, n = 40) or with low (100 mg/kg/day, n = 24) and high (1000 mg/kg/day, n = 38) doses of HET for 12 weeks. Aging animals at 48 weeks of age were used for in vitro fertilization‐embryo transfer (IVF‐ET), and their ovaries were subjected to histological and quantitative inflammation analyses. Results HET administration decreased transcript levels of ovarian inflammatory markers, interleukin 6 (IL‐6), IL‐1β, tumor necrosis factor (TNF)‐α, and interferon‐gamma (IFN‐γ) but suppressed ovulation rates and the number of ovulated oocytes in aging mice. Furthermore, HET treatment decreased the rates of oocytes maturation and fertilization and the cumulus‐cell expression of TNF‐α‐induced protein 6 and epidermal growth factor receptor. After IVF‐ET, no improvement of declined live offspring rate by aging was achieved by HET administration, although there were no adverse effects on embryo development and implantation as well as gross morphology and bodyweight of pups. Conclusion Present study indicated HET treatment interfered with ovulation and fertilization in aging mice without affecting ovarian follicle development. No improvement on the age‐associated decline of live offspring rate and follicle development was achieved after HET treatment. |
| first_indexed | 2024-04-11T04:44:23Z |
| format | Article |
| id | doaj.art-51ac883d2c584befbe83da12c6eafaf5 |
| institution | Directory Open Access Journal |
| issn | 1445-5781 1447-0578 |
| language | English |
| last_indexed | 2024-04-11T04:44:23Z |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Reproductive Medicine and Biology |
| spelling | doaj.art-51ac883d2c584befbe83da12c6eafaf52022-12-27T13:54:58ZengWileyReproductive Medicine and Biology1445-57811447-05782022-01-01211n/an/a10.1002/rmb2.12425Effects of Hochuekkito, a traditional Japanese medicine (Kampo), on reproduction of aging female miceKim Cat Tuyen Vo0Yorino Sato1Yuta Kawagoe2Kazuhiro Kawamura3Graduate School of Medicine International University of Health and Welfare School of Medicine Narita‐shi JapanAdvanced Reproductive Medicine Research Center International University of Health and Welfare School of Medicine Narita‐shi JapanAdvanced Reproductive Medicine Research Center International University of Health and Welfare School of Medicine Narita‐shi JapanGraduate School of Medicine International University of Health and Welfare School of Medicine Narita‐shi JapanAbstract Purpose To determine the potentials of Hochuekkito (HET) treatment for aging infertility. Methods Mice at 36 weeks of age were fed without (control, n = 40) or with low (100 mg/kg/day, n = 24) and high (1000 mg/kg/day, n = 38) doses of HET for 12 weeks. Aging animals at 48 weeks of age were used for in vitro fertilization‐embryo transfer (IVF‐ET), and their ovaries were subjected to histological and quantitative inflammation analyses. Results HET administration decreased transcript levels of ovarian inflammatory markers, interleukin 6 (IL‐6), IL‐1β, tumor necrosis factor (TNF)‐α, and interferon‐gamma (IFN‐γ) but suppressed ovulation rates and the number of ovulated oocytes in aging mice. Furthermore, HET treatment decreased the rates of oocytes maturation and fertilization and the cumulus‐cell expression of TNF‐α‐induced protein 6 and epidermal growth factor receptor. After IVF‐ET, no improvement of declined live offspring rate by aging was achieved by HET administration, although there were no adverse effects on embryo development and implantation as well as gross morphology and bodyweight of pups. Conclusion Present study indicated HET treatment interfered with ovulation and fertilization in aging mice without affecting ovarian follicle development. No improvement on the age‐associated decline of live offspring rate and follicle development was achieved after HET treatment.https://doi.org/10.1002/rmb2.12425HochuekkitoinfertilityKampooocyte qualityovulation |
| spellingShingle | Kim Cat Tuyen Vo Yorino Sato Yuta Kawagoe Kazuhiro Kawamura Effects of Hochuekkito, a traditional Japanese medicine (Kampo), on reproduction of aging female mice Reproductive Medicine and Biology Hochuekkito infertility Kampo oocyte quality ovulation |
| title | Effects of Hochuekkito, a traditional Japanese medicine (Kampo), on reproduction of aging female mice |
| title_full | Effects of Hochuekkito, a traditional Japanese medicine (Kampo), on reproduction of aging female mice |
| title_fullStr | Effects of Hochuekkito, a traditional Japanese medicine (Kampo), on reproduction of aging female mice |
| title_full_unstemmed | Effects of Hochuekkito, a traditional Japanese medicine (Kampo), on reproduction of aging female mice |
| title_short | Effects of Hochuekkito, a traditional Japanese medicine (Kampo), on reproduction of aging female mice |
| title_sort | effects of hochuekkito a traditional japanese medicine kampo on reproduction of aging female mice |
| topic | Hochuekkito infertility Kampo oocyte quality ovulation |
| url | https://doi.org/10.1002/rmb2.12425 |
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