Antibiotic Resistance Profiles of Bacteria Isolated from Hotspot Environments in Bahir Dar City, Northwestern Ethiopia
Kindu Geta,1,2 Mulugeta Kibret2 1Department of Biology, Debre Tabor University, Debre Tabor, Ethiopia; 2Department of Biology, Bahir Dar University, Bahir Dar, EthiopiaCorrespondence: Kindu Geta, Email kindu2012@gmail.comBackground: Wastes generated from hotspot environments contain a wide range of...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Dove Medical Press
2022-06-01
|
| Series: | Journal of Multidisciplinary Healthcare |
| Subjects: | |
| Online Access: | https://www.dovepress.com/antibiotic-resistance-profiles-of-bacteria-isolated-from-hotspot-envir-peer-reviewed-fulltext-article-JMDH |
| _version_ | 1818545959535116288 |
|---|---|
| author | Geta K Kibret M |
| author_facet | Geta K Kibret M |
| author_sort | Geta K |
| collection | DOAJ |
| description | Kindu Geta,1,2 Mulugeta Kibret2 1Department of Biology, Debre Tabor University, Debre Tabor, Ethiopia; 2Department of Biology, Bahir Dar University, Bahir Dar, EthiopiaCorrespondence: Kindu Geta, Email kindu2012@gmail.comBackground: Wastes generated from hotspot environments contain a wide range of antibiotics and pathogens that play a significant role in the dissemination of antibiotic-resistant bacteria in the environment. This study was carried out to isolate bacteria from hotspot environments and determine their resistance profiles to commonly used antibiotics in Bahir Dar city, Ethiopia.Methods: A cross-sectional study was conducted from October 2020 to June 2021 in Bahir Dar City. A total of 126 waste and wastewater samples were aseptically collected, transported, and processed for bacteriological isolation and susceptibility testing following standard procedures.Results: A total of 411 bacterial isolates were recovered and the highest value of 122 (29.7%) bacterial isolates were obtained from medical wastewater samples, and the most frequently isolated bacteria were assigned to the species Escherichia coli with 82 strains (19.5%). The results revealed that the highest resistance profile of 69 (95.8%) was obtained in Staphylococcus aureus against ampicillin and 46 (86.8%) Citrobacter spp. against tetracycline. Two hundred and sixteen (52.6%) of bacteria showed multi-drug resistance and the highest multi-drug resistance was observed in Pseudomonas spp. 47 (65.3%), followed by Escherichia coli 51 (62.2%). The highest resistance profile of 12 (85.7%) and 60 (74.1%) for tetracycline were obtained from beef waste and wastewater and medical wastewater samples. The highest multi-drug resistance was recorded in isolates isolated from beef waste and wastewater samples 11 (64.7%), followed by medical wastewater samples 84 (64.1%). Even though a higher (> 0.2) multi-antibiotic resistance index was found in all hotspot environments, the highest multi-antibiotic resistance index (0.477) was recorded in bacteria isolated from medical wastewater.Conclusion: It was concluded that wastes generated from hotspot environments and released in the environment contain large numbers of antibiotic-resistant, multidrug, extensively, and pan-drug-resistant bacteria. Proper waste management strategies should be established.Graphical Abstract: Keywords: antibiotic resistance, bacteria, hotspot environments, multidrug resistance, wastewater |
| first_indexed | 2024-12-12T07:46:47Z |
| format | Article |
| id | doaj.art-51ad9021a05f45e98df65cfc34d0a086 |
| institution | Directory Open Access Journal |
| issn | 1178-2390 |
| language | English |
| last_indexed | 2024-12-12T07:46:47Z |
| publishDate | 2022-06-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Journal of Multidisciplinary Healthcare |
| spelling | doaj.art-51ad9021a05f45e98df65cfc34d0a0862022-12-22T00:32:34ZengDove Medical PressJournal of Multidisciplinary Healthcare1178-23902022-06-01Volume 151403141476170Antibiotic Resistance Profiles of Bacteria Isolated from Hotspot Environments in Bahir Dar City, Northwestern EthiopiaGeta KKibret MKindu Geta,1,2 Mulugeta Kibret2 1Department of Biology, Debre Tabor University, Debre Tabor, Ethiopia; 2Department of Biology, Bahir Dar University, Bahir Dar, EthiopiaCorrespondence: Kindu Geta, Email kindu2012@gmail.comBackground: Wastes generated from hotspot environments contain a wide range of antibiotics and pathogens that play a significant role in the dissemination of antibiotic-resistant bacteria in the environment. This study was carried out to isolate bacteria from hotspot environments and determine their resistance profiles to commonly used antibiotics in Bahir Dar city, Ethiopia.Methods: A cross-sectional study was conducted from October 2020 to June 2021 in Bahir Dar City. A total of 126 waste and wastewater samples were aseptically collected, transported, and processed for bacteriological isolation and susceptibility testing following standard procedures.Results: A total of 411 bacterial isolates were recovered and the highest value of 122 (29.7%) bacterial isolates were obtained from medical wastewater samples, and the most frequently isolated bacteria were assigned to the species Escherichia coli with 82 strains (19.5%). The results revealed that the highest resistance profile of 69 (95.8%) was obtained in Staphylococcus aureus against ampicillin and 46 (86.8%) Citrobacter spp. against tetracycline. Two hundred and sixteen (52.6%) of bacteria showed multi-drug resistance and the highest multi-drug resistance was observed in Pseudomonas spp. 47 (65.3%), followed by Escherichia coli 51 (62.2%). The highest resistance profile of 12 (85.7%) and 60 (74.1%) for tetracycline were obtained from beef waste and wastewater and medical wastewater samples. The highest multi-drug resistance was recorded in isolates isolated from beef waste and wastewater samples 11 (64.7%), followed by medical wastewater samples 84 (64.1%). Even though a higher (> 0.2) multi-antibiotic resistance index was found in all hotspot environments, the highest multi-antibiotic resistance index (0.477) was recorded in bacteria isolated from medical wastewater.Conclusion: It was concluded that wastes generated from hotspot environments and released in the environment contain large numbers of antibiotic-resistant, multidrug, extensively, and pan-drug-resistant bacteria. Proper waste management strategies should be established.Graphical Abstract: Keywords: antibiotic resistance, bacteria, hotspot environments, multidrug resistance, wastewaterhttps://www.dovepress.com/antibiotic-resistance-profiles-of-bacteria-isolated-from-hotspot-envir-peer-reviewed-fulltext-article-JMDHantibiotic resistancebacteriahotspot environmentsmultidrug resistancewastewater |
| spellingShingle | Geta K Kibret M Antibiotic Resistance Profiles of Bacteria Isolated from Hotspot Environments in Bahir Dar City, Northwestern Ethiopia Journal of Multidisciplinary Healthcare antibiotic resistance bacteria hotspot environments multidrug resistance wastewater |
| title | Antibiotic Resistance Profiles of Bacteria Isolated from Hotspot Environments in Bahir Dar City, Northwestern Ethiopia |
| title_full | Antibiotic Resistance Profiles of Bacteria Isolated from Hotspot Environments in Bahir Dar City, Northwestern Ethiopia |
| title_fullStr | Antibiotic Resistance Profiles of Bacteria Isolated from Hotspot Environments in Bahir Dar City, Northwestern Ethiopia |
| title_full_unstemmed | Antibiotic Resistance Profiles of Bacteria Isolated from Hotspot Environments in Bahir Dar City, Northwestern Ethiopia |
| title_short | Antibiotic Resistance Profiles of Bacteria Isolated from Hotspot Environments in Bahir Dar City, Northwestern Ethiopia |
| title_sort | antibiotic resistance profiles of bacteria isolated from hotspot environments in bahir dar city northwestern ethiopia |
| topic | antibiotic resistance bacteria hotspot environments multidrug resistance wastewater |
| url | https://www.dovepress.com/antibiotic-resistance-profiles-of-bacteria-isolated-from-hotspot-envir-peer-reviewed-fulltext-article-JMDH |
| work_keys_str_mv | AT getak antibioticresistanceprofilesofbacteriaisolatedfromhotspotenvironmentsinbahirdarcitynorthwesternethiopia AT kibretm antibioticresistanceprofilesofbacteriaisolatedfromhotspotenvironmentsinbahirdarcitynorthwesternethiopia |