Male Gonads Transplantation from Kadaknath Chicken to Chicken and Duck Surrogates
Transplantation of the gonadal tissue of male and female avian species, such as chicken, onto suitable surrogates and production of live offspring has been successfully demonstrated as a strategy for the conservation and re-constitution of valuable chicken germplasm. The main objective of this study...
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MDPI AG
2023-05-01
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| Series: | Genes |
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| Online Access: | https://www.mdpi.com/2073-4425/14/5/1094 |
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| author | Adnan Naim Surya Kanta Mishra Anjan Sahoo Indra mani Nath |
| author_facet | Adnan Naim Surya Kanta Mishra Anjan Sahoo Indra mani Nath |
| author_sort | Adnan Naim |
| collection | DOAJ |
| description | Transplantation of the gonadal tissue of male and female avian species, such as chicken, onto suitable surrogates and production of live offspring has been successfully demonstrated as a strategy for the conservation and re-constitution of valuable chicken germplasm. The main objective of this study was to establish and develop the male gonadal tissue transplantation technology for the conservation of the indigenous chicken germplasm. The male gonads of the Indian native chicken breed, Kadaknath (KN), were transplanted from a day-old donor to a recipient white leghorn (WL) chicken, and Khaki Campbell (KC) ducks, as surrogates. All the surgical interventions were performed under permitted general anaesthesia, and the chicks, upon recovery, were reared with and without immunosuppressant. The recipient surrogates for the donor KN gonads were housed and reared for 10–14 weeks, and post-sacrifice, developed gonadal tissues were harvested to squeeze out the fluid to perform artificial insemination (AI). The AI-entailed fertility test using the recovered seminal extract from the transplanted KN testes from both surrogate species (KC ducks and WL males), used against KN purebred females, remained very close to the percent fertility realised from purebred KN chickens (controls). These initial results revealed from this trial study suggest definitively that, Kadaknath male gonads were readily accepted and grown inside the intra- and inter-species surrogate host, WL chicken and KC ducks, demonstrating a suitable intra- and inter-species donor-host system. Furthermore, the developed transplanted male gonads of KN chicken into the surrogates were found to have the potential to fertilise the egg and give rise to pure-line KN chicks. |
| first_indexed | 2024-03-11T03:42:33Z |
| format | Article |
| id | doaj.art-51af4744878e4988ba17b5462062c725 |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-11T03:42:33Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Genes |
| spelling | doaj.art-51af4744878e4988ba17b5462062c7252023-11-18T01:30:23ZengMDPI AGGenes2073-44252023-05-01145109410.3390/genes14051094Male Gonads Transplantation from Kadaknath Chicken to Chicken and Duck SurrogatesAdnan Naim0Surya Kanta Mishra1Anjan Sahoo2Indra mani Nath3KIIT-TBI, Campus 11, KIIT University, Bhubaneswar 751024, Odisha, IndiaIndian Council of Agriculture and Research—Directorate of Poultry Research, Regional Centre, Bhubaneswar 751003, Odisha, IndiaCollege of Veterinary Science and Animal Husbandry, Orissa University of Agriculture and Technology, Bhubaneswar 751003, Odisha, IndiaCollege of Veterinary Science and Animal Husbandry, Orissa University of Agriculture and Technology, Bhubaneswar 751003, Odisha, IndiaTransplantation of the gonadal tissue of male and female avian species, such as chicken, onto suitable surrogates and production of live offspring has been successfully demonstrated as a strategy for the conservation and re-constitution of valuable chicken germplasm. The main objective of this study was to establish and develop the male gonadal tissue transplantation technology for the conservation of the indigenous chicken germplasm. The male gonads of the Indian native chicken breed, Kadaknath (KN), were transplanted from a day-old donor to a recipient white leghorn (WL) chicken, and Khaki Campbell (KC) ducks, as surrogates. All the surgical interventions were performed under permitted general anaesthesia, and the chicks, upon recovery, were reared with and without immunosuppressant. The recipient surrogates for the donor KN gonads were housed and reared for 10–14 weeks, and post-sacrifice, developed gonadal tissues were harvested to squeeze out the fluid to perform artificial insemination (AI). The AI-entailed fertility test using the recovered seminal extract from the transplanted KN testes from both surrogate species (KC ducks and WL males), used against KN purebred females, remained very close to the percent fertility realised from purebred KN chickens (controls). These initial results revealed from this trial study suggest definitively that, Kadaknath male gonads were readily accepted and grown inside the intra- and inter-species surrogate host, WL chicken and KC ducks, demonstrating a suitable intra- and inter-species donor-host system. Furthermore, the developed transplanted male gonads of KN chicken into the surrogates were found to have the potential to fertilise the egg and give rise to pure-line KN chicks.https://www.mdpi.com/2073-4425/14/5/1094Kadaknathgonadtransplantationinterspeciespoultry |
| spellingShingle | Adnan Naim Surya Kanta Mishra Anjan Sahoo Indra mani Nath Male Gonads Transplantation from Kadaknath Chicken to Chicken and Duck Surrogates Genes Kadaknath gonad transplantation interspecies poultry |
| title | Male Gonads Transplantation from Kadaknath Chicken to Chicken and Duck Surrogates |
| title_full | Male Gonads Transplantation from Kadaknath Chicken to Chicken and Duck Surrogates |
| title_fullStr | Male Gonads Transplantation from Kadaknath Chicken to Chicken and Duck Surrogates |
| title_full_unstemmed | Male Gonads Transplantation from Kadaknath Chicken to Chicken and Duck Surrogates |
| title_short | Male Gonads Transplantation from Kadaknath Chicken to Chicken and Duck Surrogates |
| title_sort | male gonads transplantation from kadaknath chicken to chicken and duck surrogates |
| topic | Kadaknath gonad transplantation interspecies poultry |
| url | https://www.mdpi.com/2073-4425/14/5/1094 |
| work_keys_str_mv | AT adnannaim malegonadstransplantationfromkadaknathchickentochickenandducksurrogates AT suryakantamishra malegonadstransplantationfromkadaknathchickentochickenandducksurrogates AT anjansahoo malegonadstransplantationfromkadaknathchickentochickenandducksurrogates AT indramaninath malegonadstransplantationfromkadaknathchickentochickenandducksurrogates |