A comprehensive analysis of potential gastric cancer prognostic biomarker ITGBL1 associated with immune infiltration and epithelial–mesenchymal transition
Abstract Background Integrin, beta-like 1 (ITGBL1) is involved in a variety of human malignancies. However, the information on the involvement of ITGBL1 in gastric carcinoma (GC) is limited. Hence, this study aimed further to explore the functions and mechanisms of ITGBL1 in GC. Methods First, multi...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2022-05-01
|
| Series: | BioMedical Engineering OnLine |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12938-022-00998-5 |
| _version_ | 1818554745823952896 |
|---|---|
| author | Zhe Wang Liu Fu Junjie Zhang Yanli Ge Cheng Guo Rui Wang Min Deng Qizhi Wang Zhirong Wang |
| author_facet | Zhe Wang Liu Fu Junjie Zhang Yanli Ge Cheng Guo Rui Wang Min Deng Qizhi Wang Zhirong Wang |
| author_sort | Zhe Wang |
| collection | DOAJ |
| description | Abstract Background Integrin, beta-like 1 (ITGBL1) is involved in a variety of human malignancies. However, the information on the involvement of ITGBL1 in gastric carcinoma (GC) is limited. Hence, this study aimed further to explore the functions and mechanisms of ITGBL1 in GC. Methods First, multiple bioinformatics databases, including Oncomine, Tumor Immune Estimation Resource, UALCAN, and Kaplan–Meier Plotter, were used to predict the expression level and prognostic value of ITGBL1, as well as its association with immune infiltration and epithelial–mesenchymal transition (EMT) in GC. Quantitative reverse transcription–polymerase chain reaction and immunohistochemical analysis were used to detect the expression of ITGBL1 in both GC tissues and cells. Then, targeted silencing of ITGBL1 in GC cells was further used to examine the biological functions of ITGBL1. Results These databases revealed that ITGBL1 was overexpressed and affected the overall survival in GC. Besides, the expression of ITGBL1 positively correlated with immune-infiltrating cells and EMT-related markers. Subsequently, molecular biology experiments verified these predictions. In GC tissues and cells, ITGBL1 was notably overexpressed. Loss-of-function studies showed that the knockdown of ITGBL1 significantly suppressed migration and invasion but promoted apoptosis in MGC803 GC cells. Furthermore, the inhibition of ITGBL1 resulted in remarkably increased protein expression levels of cadherin 1, while the expression of Vimentin, Snail, and transforming growth factor-β1 was downregulated, indicating the initiation and progression of GC caused by ITGBL1 partly via inducing EMT. Conclusions To sum up, the findings indicated that ITGBL1 acted as a valuable oncogenic factor in GC. |
| first_indexed | 2024-12-12T09:44:08Z |
| format | Article |
| id | doaj.art-51b357f4db624394bee3b28a8f732f16 |
| institution | Directory Open Access Journal |
| issn | 1475-925X |
| language | English |
| last_indexed | 2024-12-12T09:44:08Z |
| publishDate | 2022-05-01 |
| publisher | BMC |
| record_format | Article |
| series | BioMedical Engineering OnLine |
| spelling | doaj.art-51b357f4db624394bee3b28a8f732f162022-12-22T00:28:27ZengBMCBioMedical Engineering OnLine1475-925X2022-05-0121111710.1186/s12938-022-00998-5A comprehensive analysis of potential gastric cancer prognostic biomarker ITGBL1 associated with immune infiltration and epithelial–mesenchymal transitionZhe Wang0Liu Fu1Junjie Zhang2Yanli Ge3Cheng Guo4Rui Wang5Min Deng6Qizhi Wang7Zhirong Wang8Department of Gastroenterology, Tongji Hospital, Tongji University School of MedicineDepartment of Gastroenterology, Putuo People’s Hospital, Tongji UniversityDepartment of Gastroenterology, Tongji Hospital, Tongji University School of MedicineDepartment of Gastroenterology, Tongji Hospital, Tongji University School of MedicineDepartment of Gastroenterology, Tongji Hospital, Tongji University School of MedicineDepartment of Gastroenterology, Tongji Hospital, Tongji University School of MedicineDepartment of Gastroenterology, The First Affiliated Hospital of Bengbu Medical CollegeDepartment of Gastroenterology, The First Affiliated Hospital of Bengbu Medical CollegeDepartment of Gastroenterology, Tongji Hospital, Tongji University School of MedicineAbstract Background Integrin, beta-like 1 (ITGBL1) is involved in a variety of human malignancies. However, the information on the involvement of ITGBL1 in gastric carcinoma (GC) is limited. Hence, this study aimed further to explore the functions and mechanisms of ITGBL1 in GC. Methods First, multiple bioinformatics databases, including Oncomine, Tumor Immune Estimation Resource, UALCAN, and Kaplan–Meier Plotter, were used to predict the expression level and prognostic value of ITGBL1, as well as its association with immune infiltration and epithelial–mesenchymal transition (EMT) in GC. Quantitative reverse transcription–polymerase chain reaction and immunohistochemical analysis were used to detect the expression of ITGBL1 in both GC tissues and cells. Then, targeted silencing of ITGBL1 in GC cells was further used to examine the biological functions of ITGBL1. Results These databases revealed that ITGBL1 was overexpressed and affected the overall survival in GC. Besides, the expression of ITGBL1 positively correlated with immune-infiltrating cells and EMT-related markers. Subsequently, molecular biology experiments verified these predictions. In GC tissues and cells, ITGBL1 was notably overexpressed. Loss-of-function studies showed that the knockdown of ITGBL1 significantly suppressed migration and invasion but promoted apoptosis in MGC803 GC cells. Furthermore, the inhibition of ITGBL1 resulted in remarkably increased protein expression levels of cadherin 1, while the expression of Vimentin, Snail, and transforming growth factor-β1 was downregulated, indicating the initiation and progression of GC caused by ITGBL1 partly via inducing EMT. Conclusions To sum up, the findings indicated that ITGBL1 acted as a valuable oncogenic factor in GC.https://doi.org/10.1186/s12938-022-00998-5BioinformaticsBiomarkerEpithelial–mesenchymal transitionGastric cancerImmune infiltrationITGBL1 |
| spellingShingle | Zhe Wang Liu Fu Junjie Zhang Yanli Ge Cheng Guo Rui Wang Min Deng Qizhi Wang Zhirong Wang A comprehensive analysis of potential gastric cancer prognostic biomarker ITGBL1 associated with immune infiltration and epithelial–mesenchymal transition BioMedical Engineering OnLine Bioinformatics Biomarker Epithelial–mesenchymal transition Gastric cancer Immune infiltration ITGBL1 |
| title | A comprehensive analysis of potential gastric cancer prognostic biomarker ITGBL1 associated with immune infiltration and epithelial–mesenchymal transition |
| title_full | A comprehensive analysis of potential gastric cancer prognostic biomarker ITGBL1 associated with immune infiltration and epithelial–mesenchymal transition |
| title_fullStr | A comprehensive analysis of potential gastric cancer prognostic biomarker ITGBL1 associated with immune infiltration and epithelial–mesenchymal transition |
| title_full_unstemmed | A comprehensive analysis of potential gastric cancer prognostic biomarker ITGBL1 associated with immune infiltration and epithelial–mesenchymal transition |
| title_short | A comprehensive analysis of potential gastric cancer prognostic biomarker ITGBL1 associated with immune infiltration and epithelial–mesenchymal transition |
| title_sort | comprehensive analysis of potential gastric cancer prognostic biomarker itgbl1 associated with immune infiltration and epithelial mesenchymal transition |
| topic | Bioinformatics Biomarker Epithelial–mesenchymal transition Gastric cancer Immune infiltration ITGBL1 |
| url | https://doi.org/10.1186/s12938-022-00998-5 |
| work_keys_str_mv | AT zhewang acomprehensiveanalysisofpotentialgastriccancerprognosticbiomarkeritgbl1associatedwithimmuneinfiltrationandepithelialmesenchymaltransition AT liufu acomprehensiveanalysisofpotentialgastriccancerprognosticbiomarkeritgbl1associatedwithimmuneinfiltrationandepithelialmesenchymaltransition AT junjiezhang acomprehensiveanalysisofpotentialgastriccancerprognosticbiomarkeritgbl1associatedwithimmuneinfiltrationandepithelialmesenchymaltransition AT yanlige acomprehensiveanalysisofpotentialgastriccancerprognosticbiomarkeritgbl1associatedwithimmuneinfiltrationandepithelialmesenchymaltransition AT chengguo acomprehensiveanalysisofpotentialgastriccancerprognosticbiomarkeritgbl1associatedwithimmuneinfiltrationandepithelialmesenchymaltransition AT ruiwang acomprehensiveanalysisofpotentialgastriccancerprognosticbiomarkeritgbl1associatedwithimmuneinfiltrationandepithelialmesenchymaltransition AT mindeng acomprehensiveanalysisofpotentialgastriccancerprognosticbiomarkeritgbl1associatedwithimmuneinfiltrationandepithelialmesenchymaltransition AT qizhiwang acomprehensiveanalysisofpotentialgastriccancerprognosticbiomarkeritgbl1associatedwithimmuneinfiltrationandepithelialmesenchymaltransition AT zhirongwang acomprehensiveanalysisofpotentialgastriccancerprognosticbiomarkeritgbl1associatedwithimmuneinfiltrationandepithelialmesenchymaltransition AT zhewang comprehensiveanalysisofpotentialgastriccancerprognosticbiomarkeritgbl1associatedwithimmuneinfiltrationandepithelialmesenchymaltransition AT liufu comprehensiveanalysisofpotentialgastriccancerprognosticbiomarkeritgbl1associatedwithimmuneinfiltrationandepithelialmesenchymaltransition AT junjiezhang comprehensiveanalysisofpotentialgastriccancerprognosticbiomarkeritgbl1associatedwithimmuneinfiltrationandepithelialmesenchymaltransition AT yanlige comprehensiveanalysisofpotentialgastriccancerprognosticbiomarkeritgbl1associatedwithimmuneinfiltrationandepithelialmesenchymaltransition AT chengguo comprehensiveanalysisofpotentialgastriccancerprognosticbiomarkeritgbl1associatedwithimmuneinfiltrationandepithelialmesenchymaltransition AT ruiwang comprehensiveanalysisofpotentialgastriccancerprognosticbiomarkeritgbl1associatedwithimmuneinfiltrationandepithelialmesenchymaltransition AT mindeng comprehensiveanalysisofpotentialgastriccancerprognosticbiomarkeritgbl1associatedwithimmuneinfiltrationandepithelialmesenchymaltransition AT qizhiwang comprehensiveanalysisofpotentialgastriccancerprognosticbiomarkeritgbl1associatedwithimmuneinfiltrationandepithelialmesenchymaltransition AT zhirongwang comprehensiveanalysisofpotentialgastriccancerprognosticbiomarkeritgbl1associatedwithimmuneinfiltrationandepithelialmesenchymaltransition |