Comparison of DNA extraction methods for detection of citrus huanglongbing in Colombia
Four DNA citrus plant tissue extraction protocols and three methods of DNA extraction from vector psyllid Diaphorina citri Kuwayama (Hemiptera: Psyllidae) were compared as part of the validation process and standardization for detection of huanglongbing (HLB). The comparison was done using several c...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Centro Editorial of Facultad de Ciencias Agrarias, Universidad Nacional de Colombia
2014-04-01
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| Series: | Agronomía Colombiana |
| Subjects: | |
| Online Access: | http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-99652014000100002&lng=en&tlng=en |
| _version_ | 1819139015949942784 |
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| author | Jorge Evelio Ángel Erick Geovanni Hernández Néstor Andrés Herrera Linda Yhiset Gómez Ángela Patricia Castro Sepúlveda Adriana Milena Everth Emilio Ebratt |
| author_facet | Jorge Evelio Ángel Erick Geovanni Hernández Néstor Andrés Herrera Linda Yhiset Gómez Ángela Patricia Castro Sepúlveda Adriana Milena Everth Emilio Ebratt |
| author_sort | Jorge Evelio Ángel |
| collection | DOAJ |
| description | Four DNA citrus plant tissue extraction protocols and three methods of DNA extraction from vector psyllid Diaphorina citri Kuwayama (Hemiptera: Psyllidae) were compared as part of the validation process and standardization for detection of huanglongbing (HLB). The comparison was done using several criterias such as integrity, purity and concentration. The best quality parameters presented in terms of extraction of DNA from plant midribs tissue of citrus, were cited by Murray and Thompson (1980) and Rodríguez et al. (2010), while for the DNA extraction from psyllid vectors of HLB, the best extraction method was suggested by Manjunath et al.(2008). |
| first_indexed | 2024-12-22T11:15:57Z |
| format | Article |
| id | doaj.art-51b534cf20004d7e8ae05f3a0405923b |
| institution | Directory Open Access Journal |
| issn | 0120-9965 |
| language | English |
| last_indexed | 2024-12-22T11:15:57Z |
| publishDate | 2014-04-01 |
| publisher | Centro Editorial of Facultad de Ciencias Agrarias, Universidad Nacional de Colombia |
| record_format | Article |
| series | Agronomía Colombiana |
| spelling | doaj.art-51b534cf20004d7e8ae05f3a0405923b2022-12-21T18:28:00ZengCentro Editorial of Facultad de Ciencias Agrarias, Universidad Nacional de ColombiaAgronomía Colombiana0120-99652014-04-0132171310.15446/agron.colomb.v32n1.40544S0120-99652014000100002Comparison of DNA extraction methods for detection of citrus huanglongbing in ColombiaJorge Evelio Ángel0Erick Geovanni Hernández1Néstor Andrés Herrera2Linda Yhiset Gómez3Ángela Patricia Castro4Sepúlveda Adriana Milena5Everth Emilio Ebratt6Instituto Colombiano Agropecuario (ICA)Instituto Colombiano Agropecuario (ICA)Instituto Colombiano Agropecuario (ICA)Instituto Colombiano Agropecuario (ICA)Instituto Colombiano Agropecuario (ICA)Instituto Colombiano Agropecuario (ICA)Instituto Colombiano Agropecuario (ICA)Four DNA citrus plant tissue extraction protocols and three methods of DNA extraction from vector psyllid Diaphorina citri Kuwayama (Hemiptera: Psyllidae) were compared as part of the validation process and standardization for detection of huanglongbing (HLB). The comparison was done using several criterias such as integrity, purity and concentration. The best quality parameters presented in terms of extraction of DNA from plant midribs tissue of citrus, were cited by Murray and Thompson (1980) and Rodríguez et al. (2010), while for the DNA extraction from psyllid vectors of HLB, the best extraction method was suggested by Manjunath et al.(2008).http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-99652014000100002&lng=en&tlng=enCandidatus LiberibacterDiaphorina citriPsyllidaevectores |
| spellingShingle | Jorge Evelio Ángel Erick Geovanni Hernández Néstor Andrés Herrera Linda Yhiset Gómez Ángela Patricia Castro Sepúlveda Adriana Milena Everth Emilio Ebratt Comparison of DNA extraction methods for detection of citrus huanglongbing in Colombia Agronomía Colombiana Candidatus Liberibacter Diaphorina citri Psyllidae vectores |
| title | Comparison of DNA extraction methods for detection of citrus huanglongbing in Colombia |
| title_full | Comparison of DNA extraction methods for detection of citrus huanglongbing in Colombia |
| title_fullStr | Comparison of DNA extraction methods for detection of citrus huanglongbing in Colombia |
| title_full_unstemmed | Comparison of DNA extraction methods for detection of citrus huanglongbing in Colombia |
| title_short | Comparison of DNA extraction methods for detection of citrus huanglongbing in Colombia |
| title_sort | comparison of dna extraction methods for detection of citrus huanglongbing in colombia |
| topic | Candidatus Liberibacter Diaphorina citri Psyllidae vectores |
| url | http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-99652014000100002&lng=en&tlng=en |
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