Using non-destructive sampling to evaluate the population genomic status of captive Brown Eared Pheasants
Evaluating the genetic status of threatened species is an essential task in conservation genetics. However, the genetic status of threatened species has been mostly evaluated through techniques that fail to estimate genetic diversity at the whole genomic level. Next generation sequencing can meet th...
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KeAi Communications Co., Ltd.
2023-01-01
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| Series: | Avian Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S205371662300004X |
| _version_ | 1811172023586521088 |
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| author | Pengcheng Wang Ping Hu Jinping Zhang Lixia Zhang Jing Zhang Zhengwang Zhang |
| author_facet | Pengcheng Wang Ping Hu Jinping Zhang Lixia Zhang Jing Zhang Zhengwang Zhang |
| author_sort | Pengcheng Wang |
| collection | DOAJ |
| description | Evaluating the genetic status of threatened species is an essential task in conservation genetics. However, the genetic status of threatened species has been mostly evaluated through techniques that fail to estimate genetic diversity at the whole genomic level. Next generation sequencing can meet this demand, but high quality samples such as blood or muscle tissues are required. However, it is difficult to collect such samples from threatened species because sampling work may impact their health. Therefore, it is essential to design a workflow to evaluate the whole genomic status of threatened species using non-destructive sampling. Even though non-destructive sampling has been used in traditional barcoding technique, the barcoding technique cannot evaluate the whole genomic status. Brown Eared Pheasant (Crossoptilon mantchuricum) is an endangered species, with captive populations maintained in Taiyuan Zoo, China, and Europe. However, the genetic diversity, inbreeding pattern, and mutation load of these two populations are unclear. To uncover the genetic status of these two captive populations, we applied 2b-RAD technology to evaluate the genomic status of these populations using feathers as samples. The feathers could be collected by non-destructive sampling. The results indicate that the Taiyuan Zoo population has a lower genetic diversity and higher inbreeding coefficient than the European population. The Taiyuan Zoo population has lethal mutations when homozygous. The current project uses a non-destructive sampling technique to evaluate the whole genomic status of the two captive populations, providing a paradigm for conservation genetics, which will facilitate the development of conservation biology. |
| first_indexed | 2024-04-10T17:23:37Z |
| format | Article |
| id | doaj.art-51b5db8d547645f381df0db6bb75bfef |
| institution | Directory Open Access Journal |
| issn | 2053-7166 |
| language | English |
| last_indexed | 2024-04-10T17:23:37Z |
| publishDate | 2023-01-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Avian Research |
| spelling | doaj.art-51b5db8d547645f381df0db6bb75bfef2023-02-05T04:15:45ZengKeAi Communications Co., Ltd.Avian Research2053-71662023-01-0114100078Using non-destructive sampling to evaluate the population genomic status of captive Brown Eared PheasantsPengcheng Wang0Ping Hu1Jinping Zhang2Lixia Zhang3Jing Zhang4Zhengwang Zhang5Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing, 210023, ChinaJiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing, 210023, ChinaJiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing, 210023, ChinaTaiyuan Zoo, Taiyuan, 030009, ChinaBeijing Zoo Management Office, Beijing Key Laboratory of Captive Wildlife Technologies of Beijing Zoo, Beijing, 100044, China; Corresponding author.Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering, College of Life Sciences, Beijing Normal University, Beijing, 100875, China; Corresponding author.Evaluating the genetic status of threatened species is an essential task in conservation genetics. However, the genetic status of threatened species has been mostly evaluated through techniques that fail to estimate genetic diversity at the whole genomic level. Next generation sequencing can meet this demand, but high quality samples such as blood or muscle tissues are required. However, it is difficult to collect such samples from threatened species because sampling work may impact their health. Therefore, it is essential to design a workflow to evaluate the whole genomic status of threatened species using non-destructive sampling. Even though non-destructive sampling has been used in traditional barcoding technique, the barcoding technique cannot evaluate the whole genomic status. Brown Eared Pheasant (Crossoptilon mantchuricum) is an endangered species, with captive populations maintained in Taiyuan Zoo, China, and Europe. However, the genetic diversity, inbreeding pattern, and mutation load of these two populations are unclear. To uncover the genetic status of these two captive populations, we applied 2b-RAD technology to evaluate the genomic status of these populations using feathers as samples. The feathers could be collected by non-destructive sampling. The results indicate that the Taiyuan Zoo population has a lower genetic diversity and higher inbreeding coefficient than the European population. The Taiyuan Zoo population has lethal mutations when homozygous. The current project uses a non-destructive sampling technique to evaluate the whole genomic status of the two captive populations, providing a paradigm for conservation genetics, which will facilitate the development of conservation biology.http://www.sciencedirect.com/science/article/pii/S205371662300004XGenetic diversityInbreeding coefficientKinshipMutation loadThreatened species |
| spellingShingle | Pengcheng Wang Ping Hu Jinping Zhang Lixia Zhang Jing Zhang Zhengwang Zhang Using non-destructive sampling to evaluate the population genomic status of captive Brown Eared Pheasants Avian Research Genetic diversity Inbreeding coefficient Kinship Mutation load Threatened species |
| title | Using non-destructive sampling to evaluate the population genomic status of captive Brown Eared Pheasants |
| title_full | Using non-destructive sampling to evaluate the population genomic status of captive Brown Eared Pheasants |
| title_fullStr | Using non-destructive sampling to evaluate the population genomic status of captive Brown Eared Pheasants |
| title_full_unstemmed | Using non-destructive sampling to evaluate the population genomic status of captive Brown Eared Pheasants |
| title_short | Using non-destructive sampling to evaluate the population genomic status of captive Brown Eared Pheasants |
| title_sort | using non destructive sampling to evaluate the population genomic status of captive brown eared pheasants |
| topic | Genetic diversity Inbreeding coefficient Kinship Mutation load Threatened species |
| url | http://www.sciencedirect.com/science/article/pii/S205371662300004X |
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