Comparison of Minced Cartilage Implantation with Autologous Chondrocyte Transplantation in an In Vitro Inflammation Model
The current gold standard to treat large cartilage defects is autologous chondrocyte transplantation (ACT). As a new surgical method of cartilage regeneration, minced cartilage implantation (MCI) is increasingly coming into focus. The aim of this study is to investigate the influence of chondrogenes...
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MDPI AG
2024-03-01
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| Online Access: | https://www.mdpi.com/2073-4409/13/6/546 |
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| author | Robert Ossendorff Lisa Grede Sebastian Scheidt Andreas C. Strauss Christof Burger Dieter C. Wirtz Gian M. Salzmann Frank A. Schildberg |
| author_facet | Robert Ossendorff Lisa Grede Sebastian Scheidt Andreas C. Strauss Christof Burger Dieter C. Wirtz Gian M. Salzmann Frank A. Schildberg |
| author_sort | Robert Ossendorff |
| collection | DOAJ |
| description | The current gold standard to treat large cartilage defects is autologous chondrocyte transplantation (ACT). As a new surgical method of cartilage regeneration, minced cartilage implantation (MCI) is increasingly coming into focus. The aim of this study is to investigate the influence of chondrogenesis between isolated and cultured chondrocytes compared to cartilage chips in a standardized inflammation model with the proinflammatory cytokine TNFα. Articular chondrocytes from bovine cartilage were cultured according to the ACT method to passage 3 and transferred to spheroid culture. At the same time, cartilage was fragmented (<1 mm<sup>3</sup>) to produce cartilage chips. TNFα (20 ng/mL) was supplemented to simulate an inflammatory process. TNFα had a stronger influence on the passaged chondrocytes compared to the non-passaged ones, affecting gene expression profiles differently between isolated chondrocytes and cartilage chips. MCI showed less susceptibility to TNFα, with reduced IL-6 release and less impact on inflammation markers. Biochemical and histological analyses supported these findings, showing a greater negative influence of TNFα on the passaged pellet cultures compared to the unpassaged cells and MCI constructs. This study demonstrated the negative influence of TNFα on chondrogenesis in a chondrocyte spheroid culture and cartilage fragment model. Passaged chondrocytes are more sensitive to cytokine influences compared to non-passaged cells and chondrons. This suggests that MCI may have superior regeneration potential in osteoarthritic conditions compared to ACT. Further investigations are necessary for the translation of these findings into clinical practice. |
| first_indexed | 2024-04-24T18:26:42Z |
| format | Article |
| id | doaj.art-51b9c90291f14584bcaa439e9cc759b4 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-04-24T18:26:42Z |
| publishDate | 2024-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-51b9c90291f14584bcaa439e9cc759b42024-03-27T13:30:43ZengMDPI AGCells2073-44092024-03-0113654610.3390/cells13060546Comparison of Minced Cartilage Implantation with Autologous Chondrocyte Transplantation in an In Vitro Inflammation ModelRobert Ossendorff0Lisa Grede1Sebastian Scheidt2Andreas C. Strauss3Christof Burger4Dieter C. Wirtz5Gian M. Salzmann6Frank A. Schildberg7Department of Orthopedics and Trauma Surgery, University Hospital Bonn, 53127 Bonn, GermanyDepartment of Orthopedics and Trauma Surgery, University Hospital Bonn, 53127 Bonn, GermanyDepartment of Orthopedics and Trauma Surgery, University Hospital Bonn, 53127 Bonn, GermanyDepartment of Orthopedics and Trauma Surgery, University Hospital Bonn, 53127 Bonn, GermanyDepartment of Orthopedics and Trauma Surgery, University Hospital Bonn, 53127 Bonn, GermanyDepartment of Orthopedics and Trauma Surgery, University Hospital Bonn, 53127 Bonn, GermanyGelenkzentrum Rhein-Main, 65239 Hochheim, GermanyDepartment of Orthopedics and Trauma Surgery, University Hospital Bonn, 53127 Bonn, GermanyThe current gold standard to treat large cartilage defects is autologous chondrocyte transplantation (ACT). As a new surgical method of cartilage regeneration, minced cartilage implantation (MCI) is increasingly coming into focus. The aim of this study is to investigate the influence of chondrogenesis between isolated and cultured chondrocytes compared to cartilage chips in a standardized inflammation model with the proinflammatory cytokine TNFα. Articular chondrocytes from bovine cartilage were cultured according to the ACT method to passage 3 and transferred to spheroid culture. At the same time, cartilage was fragmented (<1 mm<sup>3</sup>) to produce cartilage chips. TNFα (20 ng/mL) was supplemented to simulate an inflammatory process. TNFα had a stronger influence on the passaged chondrocytes compared to the non-passaged ones, affecting gene expression profiles differently between isolated chondrocytes and cartilage chips. MCI showed less susceptibility to TNFα, with reduced IL-6 release and less impact on inflammation markers. Biochemical and histological analyses supported these findings, showing a greater negative influence of TNFα on the passaged pellet cultures compared to the unpassaged cells and MCI constructs. This study demonstrated the negative influence of TNFα on chondrogenesis in a chondrocyte spheroid culture and cartilage fragment model. Passaged chondrocytes are more sensitive to cytokine influences compared to non-passaged cells and chondrons. This suggests that MCI may have superior regeneration potential in osteoarthritic conditions compared to ACT. Further investigations are necessary for the translation of these findings into clinical practice.https://www.mdpi.com/2073-4409/13/6/546autologous chondrocyte transplantationminced cartilage implantationinflammation modeltumor necrosis factor αmusculoskeletal immunology |
| spellingShingle | Robert Ossendorff Lisa Grede Sebastian Scheidt Andreas C. Strauss Christof Burger Dieter C. Wirtz Gian M. Salzmann Frank A. Schildberg Comparison of Minced Cartilage Implantation with Autologous Chondrocyte Transplantation in an In Vitro Inflammation Model Cells autologous chondrocyte transplantation minced cartilage implantation inflammation model tumor necrosis factor α musculoskeletal immunology |
| title | Comparison of Minced Cartilage Implantation with Autologous Chondrocyte Transplantation in an In Vitro Inflammation Model |
| title_full | Comparison of Minced Cartilage Implantation with Autologous Chondrocyte Transplantation in an In Vitro Inflammation Model |
| title_fullStr | Comparison of Minced Cartilage Implantation with Autologous Chondrocyte Transplantation in an In Vitro Inflammation Model |
| title_full_unstemmed | Comparison of Minced Cartilage Implantation with Autologous Chondrocyte Transplantation in an In Vitro Inflammation Model |
| title_short | Comparison of Minced Cartilage Implantation with Autologous Chondrocyte Transplantation in an In Vitro Inflammation Model |
| title_sort | comparison of minced cartilage implantation with autologous chondrocyte transplantation in an in vitro inflammation model |
| topic | autologous chondrocyte transplantation minced cartilage implantation inflammation model tumor necrosis factor α musculoskeletal immunology |
| url | https://www.mdpi.com/2073-4409/13/6/546 |
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