Remifentanil reduces glutamate toxicity in rat olfactory bulb neurons in culture
Background: Opioids are widely used as an analgesic drug in the surgical setting. Remifentanil is an ultra-short acting opioid with selective affinity to the mu (μ) receptor, and also exhibits GABA agonist effects. The aim of this study was study of the neurotoxic or neuroprotective effect of differ...
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| Format: | Article |
| Language: | English |
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Elsevier
2021-07-01
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| Series: | Brazilian Journal of Anesthesiology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0104001421001536 |
| _version_ | 1811199803185430528 |
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| author | Muhammet Emin Naldan Ali Taghizadehghalehjoughi |
| author_facet | Muhammet Emin Naldan Ali Taghizadehghalehjoughi |
| author_sort | Muhammet Emin Naldan |
| collection | DOAJ |
| description | Background: Opioids are widely used as an analgesic drug in the surgical setting. Remifentanil is an ultra-short acting opioid with selective affinity to the mu (μ) receptor, and also exhibits GABA agonist effects. The aim of this study was study of the neurotoxic or neuroprotective effect of different doses of remifentanil in glutamate-induced toxicity in olfactory neuron cell culture. Materials and methods: Olfactory neurons were obtained from newborn Sprague Dawley rat pups. Glutamate 10-5 mM was added to all culture dishes, except for the negative control group. Remifentanil was added at three different doses for 24 hours, after which evaluation was performed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), Total Antioxidant Capacity (TAC), Total Oxidant Status (TOS), and Annexin V. Results: The highest and lowest viability values were obtained from the low and high remifentanil doses at approximately 91% and 75%, respectively. TAC and TOS were correlated with the MTT results. TAC, TOS and MTT most closely approximated to the sham group values in the remifentanil 0.02 mM group. Conclusions: Our results suggest that remifentanil has the potential to reduce glutamate toxicity and to increase cell viability in cultured neuron from the rat olfactory bulb. |
| first_indexed | 2024-04-12T01:54:40Z |
| format | Article |
| id | doaj.art-51c506278ae94eea915e29fa0ad027d0 |
| institution | Directory Open Access Journal |
| issn | 0104-0014 |
| language | English |
| last_indexed | 2024-04-12T01:54:40Z |
| publishDate | 2021-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Brazilian Journal of Anesthesiology |
| spelling | doaj.art-51c506278ae94eea915e29fa0ad027d02022-12-22T03:52:50ZengElsevierBrazilian Journal of Anesthesiology0104-00142021-07-01714402407Remifentanil reduces glutamate toxicity in rat olfactory bulb neurons in cultureMuhammet Emin Naldan0Ali Taghizadehghalehjoughi1Erzurum Regional Training and Research Hospital, Department of Anesthesiology and Reanimation, Erzurum, Turkey; Corresponding author.Atatürk University, Faculty of Veterinary Science, Department of Pharmacology and Toxicology, Erzurum, TurkeyBackground: Opioids are widely used as an analgesic drug in the surgical setting. Remifentanil is an ultra-short acting opioid with selective affinity to the mu (μ) receptor, and also exhibits GABA agonist effects. The aim of this study was study of the neurotoxic or neuroprotective effect of different doses of remifentanil in glutamate-induced toxicity in olfactory neuron cell culture. Materials and methods: Olfactory neurons were obtained from newborn Sprague Dawley rat pups. Glutamate 10-5 mM was added to all culture dishes, except for the negative control group. Remifentanil was added at three different doses for 24 hours, after which evaluation was performed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), Total Antioxidant Capacity (TAC), Total Oxidant Status (TOS), and Annexin V. Results: The highest and lowest viability values were obtained from the low and high remifentanil doses at approximately 91% and 75%, respectively. TAC and TOS were correlated with the MTT results. TAC, TOS and MTT most closely approximated to the sham group values in the remifentanil 0.02 mM group. Conclusions: Our results suggest that remifentanil has the potential to reduce glutamate toxicity and to increase cell viability in cultured neuron from the rat olfactory bulb.http://www.sciencedirect.com/science/article/pii/S0104001421001536RemifentanilOlfactory bulb neuronGlutamateNeurotoxicity |
| spellingShingle | Muhammet Emin Naldan Ali Taghizadehghalehjoughi Remifentanil reduces glutamate toxicity in rat olfactory bulb neurons in culture Brazilian Journal of Anesthesiology Remifentanil Olfactory bulb neuron Glutamate Neurotoxicity |
| title | Remifentanil reduces glutamate toxicity in rat olfactory bulb neurons in culture |
| title_full | Remifentanil reduces glutamate toxicity in rat olfactory bulb neurons in culture |
| title_fullStr | Remifentanil reduces glutamate toxicity in rat olfactory bulb neurons in culture |
| title_full_unstemmed | Remifentanil reduces glutamate toxicity in rat olfactory bulb neurons in culture |
| title_short | Remifentanil reduces glutamate toxicity in rat olfactory bulb neurons in culture |
| title_sort | remifentanil reduces glutamate toxicity in rat olfactory bulb neurons in culture |
| topic | Remifentanil Olfactory bulb neuron Glutamate Neurotoxicity |
| url | http://www.sciencedirect.com/science/article/pii/S0104001421001536 |
| work_keys_str_mv | AT muhammeteminnaldan remifentanilreducesglutamatetoxicityinratolfactorybulbneuronsinculture AT alitaghizadehghalehjoughi remifentanilreducesglutamatetoxicityinratolfactorybulbneuronsinculture |