Plasma exosomal miR-596: a novel biomarker predicts survival in patients with idiopathic pulmonary artery hypertension
Objective To determine if plasma exosomal microRNAs (miRNAs) can predict survival in patients with idiopathic pulmonary arterial hypertension (IPAH). Methods The study enrolled patients with IPAH that underwent right heart catheterization. Plasma was collected and exosomal miRNAs were extracted. Exo...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
SAGE Publishing
2021-03-01
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| Series: | Journal of International Medical Research |
| Online Access: | https://doi.org/10.1177/03000605211002379 |
| _version_ | 1818402585878462464 |
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| author | Yang Huang Zuo-Gang Wang Liang Tang Su-Gang Gong Yuan-Yuan Sun Lan Wang Rong Jiang Wen-Hui Wu Ci-Jun Luo Jie Zhang Xiao-Jun Yang Jin-Ling Li Xun-Tao Yuan Qin-Hua Zhao Ping Yuan |
| author_facet | Yang Huang Zuo-Gang Wang Liang Tang Su-Gang Gong Yuan-Yuan Sun Lan Wang Rong Jiang Wen-Hui Wu Ci-Jun Luo Jie Zhang Xiao-Jun Yang Jin-Ling Li Xun-Tao Yuan Qin-Hua Zhao Ping Yuan |
| author_sort | Yang Huang |
| collection | DOAJ |
| description | Objective To determine if plasma exosomal microRNAs (miRNAs) can predict survival in patients with idiopathic pulmonary arterial hypertension (IPAH). Methods The study enrolled patients with IPAH that underwent right heart catheterization. Plasma was collected and exosomal miRNAs were extracted. Exosomes were evaluated using transmission electron microscopy, Western blot analysis and particle size distribution analysis. MiRNAs were evaluated using a miRNA microarray and validated using real-time polymerase chain reaction. Results This study included 12 patients with IPAH in the study group and 48 patients with IPAH in the validation group. The mean ± SD follow-up duration was 60.3 ± 35.4 months in the overall cohort. The levels of miR-596 were higher in the nonsurvivors compared with the survivors. The levels of miR-596 significantly correlated with survival time, mean right atrial pressure, pulmonary vascular resistance (PVR) and cardiac index. High levels of miR-596 and PVR were significantly associated with poor overall survival. Multivariate analysis demonstrated that exosomal miR-596 (hazard ratio [HR] = 2.119; 95% confidence interval [CI] 1.402, 3.203) and PVR (HR = 1.146; 95% CI 1.010, 1.300) were independent predictors of survival. Conclusions High levels of plasma exosomal miR-596 were significantly associated with disease severity and poor prognosis of patients with IPAH. |
| first_indexed | 2024-12-14T08:10:43Z |
| format | Article |
| id | doaj.art-51c572feb010493595e2dfc2aab7340c |
| institution | Directory Open Access Journal |
| issn | 1473-2300 |
| language | English |
| last_indexed | 2024-12-14T08:10:43Z |
| publishDate | 2021-03-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Journal of International Medical Research |
| spelling | doaj.art-51c572feb010493595e2dfc2aab7340c2022-12-21T23:10:05ZengSAGE PublishingJournal of International Medical Research1473-23002021-03-014910.1177/03000605211002379Plasma exosomal miR-596: a novel biomarker predicts survival in patients with idiopathic pulmonary artery hypertensionYang HuangZuo-Gang WangLiang TangSu-Gang GongYuan-Yuan SunLan WangRong JiangWen-Hui WuCi-Jun LuoJie ZhangXiao-Jun YangJin-Ling LiXun-Tao YuanQin-Hua ZhaoPing YuanObjective To determine if plasma exosomal microRNAs (miRNAs) can predict survival in patients with idiopathic pulmonary arterial hypertension (IPAH). Methods The study enrolled patients with IPAH that underwent right heart catheterization. Plasma was collected and exosomal miRNAs were extracted. Exosomes were evaluated using transmission electron microscopy, Western blot analysis and particle size distribution analysis. MiRNAs were evaluated using a miRNA microarray and validated using real-time polymerase chain reaction. Results This study included 12 patients with IPAH in the study group and 48 patients with IPAH in the validation group. The mean ± SD follow-up duration was 60.3 ± 35.4 months in the overall cohort. The levels of miR-596 were higher in the nonsurvivors compared with the survivors. The levels of miR-596 significantly correlated with survival time, mean right atrial pressure, pulmonary vascular resistance (PVR) and cardiac index. High levels of miR-596 and PVR were significantly associated with poor overall survival. Multivariate analysis demonstrated that exosomal miR-596 (hazard ratio [HR] = 2.119; 95% confidence interval [CI] 1.402, 3.203) and PVR (HR = 1.146; 95% CI 1.010, 1.300) were independent predictors of survival. Conclusions High levels of plasma exosomal miR-596 were significantly associated with disease severity and poor prognosis of patients with IPAH.https://doi.org/10.1177/03000605211002379 |
| spellingShingle | Yang Huang Zuo-Gang Wang Liang Tang Su-Gang Gong Yuan-Yuan Sun Lan Wang Rong Jiang Wen-Hui Wu Ci-Jun Luo Jie Zhang Xiao-Jun Yang Jin-Ling Li Xun-Tao Yuan Qin-Hua Zhao Ping Yuan Plasma exosomal miR-596: a novel biomarker predicts survival in patients with idiopathic pulmonary artery hypertension Journal of International Medical Research |
| title | Plasma exosomal miR-596: a novel biomarker predicts survival in patients with idiopathic pulmonary artery hypertension |
| title_full | Plasma exosomal miR-596: a novel biomarker predicts survival in patients with idiopathic pulmonary artery hypertension |
| title_fullStr | Plasma exosomal miR-596: a novel biomarker predicts survival in patients with idiopathic pulmonary artery hypertension |
| title_full_unstemmed | Plasma exosomal miR-596: a novel biomarker predicts survival in patients with idiopathic pulmonary artery hypertension |
| title_short | Plasma exosomal miR-596: a novel biomarker predicts survival in patients with idiopathic pulmonary artery hypertension |
| title_sort | plasma exosomal mir 596 a novel biomarker predicts survival in patients with idiopathic pulmonary artery hypertension |
| url | https://doi.org/10.1177/03000605211002379 |
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