Effects of cationic polymers on the viability of microbial biofilms
Introduction: The number of published biofilm studies and novel ways for studying them has risen dramatically in recent years, ow-ing to the broad application of biofilms in medicine. Some bacteria develop biofilms that are highly resistant to antimicrobial agents, resulting in persistent infections...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Pensoft Publishers
2023-02-01
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| Series: | Folia Medica |
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| Online Access: | https://foliamedica.bg/article/76154/download/pdf/ |
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| author | Paulina Teper Anna Sotirova Agnieszka Kowalczuk Barbara Mendrek Tsvetelina Paunova-Krasteva |
| author_facet | Paulina Teper Anna Sotirova Agnieszka Kowalczuk Barbara Mendrek Tsvetelina Paunova-Krasteva |
| author_sort | Paulina Teper |
| collection | DOAJ |
| description | Introduction: The number of published biofilm studies and novel ways for studying them has risen dramatically in recent years, ow-ing to the broad application of biofilms in medicine. Some bacteria develop biofilms that are highly resistant to antimicrobial agents, resulting in persistent infections. This necessitates the development of alternative methods for combating biofilms. In this regard, the application of cationic polymers is a good candidate for realization of this strategy. Aim: The aim of our study was to investigate the potential of a newly synthesized covalently attached star copolymer of N,N’-dimeth-ylaminoethyl methacrylate and hydroxyl-bearing oligo(ethylene glycol) methacrylate [P(DMAEMA-co-HOEGMA)] to silica surfaces and its quaternized version [P(QDMAEMA-co-HOEGMA)] for destruction of biofilms formed by Bacillus subtilis or Pseudomonas aeruginosa. Materials and methods: Model strains representing different genera and taxonomic groups were selected for the study. The anti-biofilm activities of two different newly synthesized cationic polymers were investigated by observation (live/dead staining) of the viability of bacterial cells within the biofilm.Results: The results obtained by the live/dead labeling of bacterial biofilms show a substantial decrease in the viability of population in the presence of cationic polymers, better expressed at B. subtilis.Conclusions: The studied two immobilized on silica wafers newly synthesized star copolymers exhibited potential for anti-biofilm effects. The results demonstrated combined potential for reducing the viability of bacterial cells within the biofilms and probably for loosening the biofilm matrix. The effect was better expressed in B. subtilis. |
| first_indexed | 2024-04-10T06:15:02Z |
| format | Article |
| id | doaj.art-51c80a32d694426197e9db84309945d6 |
| institution | Directory Open Access Journal |
| issn | 1314-2143 |
| language | English |
| last_indexed | 2024-04-10T06:15:02Z |
| publishDate | 2023-02-01 |
| publisher | Pensoft Publishers |
| record_format | Article |
| series | Folia Medica |
| spelling | doaj.art-51c80a32d694426197e9db84309945d62023-03-02T09:11:08ZengPensoft PublishersFolia Medica1314-21432023-02-0165112413010.3897/folmed.65.e7615476154Effects of cationic polymers on the viability of microbial biofilmsPaulina Teper0Anna Sotirova1Agnieszka Kowalczuk2Barbara Mendrek3Tsvetelina Paunova-Krasteva4Centre of Polymer and Carbon Materials, Polish Academy of SciencesThe Stephan Angeloff Institute of Microbiology, Bulgarian Academy of SciencesCentre of Polymer and Carbon Materials, Polish Academy of SciencesCentre of Polymer and Carbon Materials, Polish Academy of SciencesThe Stephan Angeloff Institute of Microbiology, Bulgarian Academy of SciencesIntroduction: The number of published biofilm studies and novel ways for studying them has risen dramatically in recent years, ow-ing to the broad application of biofilms in medicine. Some bacteria develop biofilms that are highly resistant to antimicrobial agents, resulting in persistent infections. This necessitates the development of alternative methods for combating biofilms. In this regard, the application of cationic polymers is a good candidate for realization of this strategy.&nbsp;Aim: The aim of our study was to investigate the potential of a newly synthesized covalently attached star copolymer of N,N&rsquo;-dimeth-ylaminoethyl methacrylate and hydroxyl-bearing oligo(ethylene glycol) methacrylate [P(DMAEMA-co-HOEGMA)] to silica surfaces and its quaternized version [P(QDMAEMA-co-HOEGMA)] for destruction of biofilms formed by Bacillus subtilis or Pseudomonas aeruginosa.&nbsp;Materials and methods: Model strains representing different genera and taxonomic groups were selected for the study. The anti-biofilm activities of two different newly synthesized cationic polymers were investigated by observation (live/dead staining) of the viability of bacterial cells within the biofilm.Results: The results obtained by the live/dead labeling of bacterial biofilms show a substantial decrease in the viability of population in the presence of cationic polymers, better expressed at B. subtilis.Conclusions: The studied two immobilized on silica wafers newly synthesized star copolymers exhibited potential for anti-biofilm effects. The results demonstrated combined potential for reducing the viability of bacterial cells within the biofilms and probably for loosening the biofilm matrix. The effect was better expressed in B. subtilis.https://foliamedica.bg/article/76154/download/pdf/biofilm destruction<em>B. subtilis</em><em>P. |
| spellingShingle | Paulina Teper Anna Sotirova Agnieszka Kowalczuk Barbara Mendrek Tsvetelina Paunova-Krasteva Effects of cationic polymers on the viability of microbial biofilms Folia Medica biofilm destruction <em>B. subtilis</em> <em>P. |
| title | Effects of cationic polymers on the viability of microbial biofilms |
| title_full | Effects of cationic polymers on the viability of microbial biofilms |
| title_fullStr | Effects of cationic polymers on the viability of microbial biofilms |
| title_full_unstemmed | Effects of cationic polymers on the viability of microbial biofilms |
| title_short | Effects of cationic polymers on the viability of microbial biofilms |
| title_sort | effects of cationic polymers on the viability of microbial biofilms |
| topic | biofilm destruction <em>B. subtilis</em> <em>P. |
| url | https://foliamedica.bg/article/76154/download/pdf/ |
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