Effect of Cell Generations on t-BHP-induced Oxidative Stress Model of Caco-2 Cells
Objective: To investigate the effects of cell passage number on the establishment of an oxidative stress model in human colon adenocarcinoma cells (Caco-2) caused by tert-butyl hydroperoxide (t-BHP). Methods: First, by applying various t-BHP concentrations (1, 2 and 3 mmol/L) to the F15 generation o...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | zho |
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The editorial department of Science and Technology of Food Industry
2023-03-01
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| Series: | Shipin gongye ke-ji |
| Subjects: | |
| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022050222 |
| _version_ | 1811162801994989568 |
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| author | Jun NI Cailian YUAN Rong SHE Chengfa ZHAO Lijuan LI Xu YANG Xiaoyan YANG |
| author_facet | Jun NI Cailian YUAN Rong SHE Chengfa ZHAO Lijuan LI Xu YANG Xiaoyan YANG |
| author_sort | Jun NI |
| collection | DOAJ |
| description | Objective: To investigate the effects of cell passage number on the establishment of an oxidative stress model in human colon adenocarcinoma cells (Caco-2) caused by tert-butyl hydroperoxide (t-BHP). Methods: First, by applying various t-BHP concentrations (1, 2 and 3 mmol/L) to the F15 generation of Caco-2 cells for 1, 2, 3, 4, 5 and 6 hours, the content of malondialdehyde (MDA) and 8-hydroxydeoxyguanosine (8-OHdG) in different treatment groups were measured to determine the basic conditions for constructing the oxidative stress model of Caco-2 cells. Then, based on the fundamental parameters, the effect of cell passage number (F14, F15, F18, F19 and F20) on the development of oxidative stress model was investigated using the content of 8-OHdG as an index. Results: When exposed to 1, 2 and 3 mmol/L of t-BHP for 3 to 6 hours, the oxidative stress model of Caco-2 cells from the F15 generation could be established steadily. The content of 8-OHdG in Caco-2 cells of the F14, F15 and F18 generations was significantly higher than that in control group (which had not been treated with t-BHP) after being exposed to 2.0 mmol/L t-BHP for 5 hours (P<0.05), whereas there was no significant difference between the F19 and F20 generations and the control group. Conclusion: The development of the oxidative stress model of Caco-2 cells will be strongly impacted by cell passage number. By subjecting Caco-2 cells prior to F19 to 2 mmol/L t-BHP for 5 hours, the oxidative stress model may be successfully constructed. |
| first_indexed | 2024-04-10T06:35:02Z |
| format | Article |
| id | doaj.art-51c840115ed84546942927dd4f9ece3b |
| institution | Directory Open Access Journal |
| issn | 1002-0306 |
| language | zho |
| last_indexed | 2024-04-10T06:35:02Z |
| publishDate | 2023-03-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
| record_format | Article |
| series | Shipin gongye ke-ji |
| spelling | doaj.art-51c840115ed84546942927dd4f9ece3b2023-03-01T01:21:35ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062023-03-01445606610.13386/j.issn1002-0306.20220502222022050222-5Effect of Cell Generations on t-BHP-induced Oxidative Stress Model of Caco-2 CellsJun NI0Cailian YUAN1Rong SHE2Chengfa ZHAO3Lijuan LI4Xu YANG5Xiaoyan YANG6Institute of Natural Antioxidants and Anti-inflammation, Dali University, Dali 671003, ChinaInstitute of Natural Antioxidants and Anti-inflammation, Dali University, Dali 671003, ChinaInstitute of Natural Antioxidants and Anti-inflammation, Dali University, Dali 671003, ChinaInstitute of Natural Antioxidants and Anti-inflammation, Dali University, Dali 671003, ChinaInstitute of Natural Antioxidants and Anti-inflammation, Dali University, Dali 671003, ChinaInstitute of Natural Antioxidants and Anti-inflammation, Dali University, Dali 671003, ChinaInstitute of Natural Antioxidants and Anti-inflammation, Dali University, Dali 671003, ChinaObjective: To investigate the effects of cell passage number on the establishment of an oxidative stress model in human colon adenocarcinoma cells (Caco-2) caused by tert-butyl hydroperoxide (t-BHP). Methods: First, by applying various t-BHP concentrations (1, 2 and 3 mmol/L) to the F15 generation of Caco-2 cells for 1, 2, 3, 4, 5 and 6 hours, the content of malondialdehyde (MDA) and 8-hydroxydeoxyguanosine (8-OHdG) in different treatment groups were measured to determine the basic conditions for constructing the oxidative stress model of Caco-2 cells. Then, based on the fundamental parameters, the effect of cell passage number (F14, F15, F18, F19 and F20) on the development of oxidative stress model was investigated using the content of 8-OHdG as an index. Results: When exposed to 1, 2 and 3 mmol/L of t-BHP for 3 to 6 hours, the oxidative stress model of Caco-2 cells from the F15 generation could be established steadily. The content of 8-OHdG in Caco-2 cells of the F14, F15 and F18 generations was significantly higher than that in control group (which had not been treated with t-BHP) after being exposed to 2.0 mmol/L t-BHP for 5 hours (P<0.05), whereas there was no significant difference between the F19 and F20 generations and the control group. Conclusion: The development of the oxidative stress model of Caco-2 cells will be strongly impacted by cell passage number. By subjecting Caco-2 cells prior to F19 to 2 mmol/L t-BHP for 5 hours, the oxidative stress model may be successfully constructed.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022050222caco-2 cellstert-butyl hydrogen peroxideoxidative stresscell modelcell passage number8-hydroxydeoxyguanosine |
| spellingShingle | Jun NI Cailian YUAN Rong SHE Chengfa ZHAO Lijuan LI Xu YANG Xiaoyan YANG Effect of Cell Generations on t-BHP-induced Oxidative Stress Model of Caco-2 Cells Shipin gongye ke-ji caco-2 cells tert-butyl hydrogen peroxide oxidative stress cell model cell passage number 8-hydroxydeoxyguanosine |
| title | Effect of Cell Generations on t-BHP-induced Oxidative Stress Model of Caco-2 Cells |
| title_full | Effect of Cell Generations on t-BHP-induced Oxidative Stress Model of Caco-2 Cells |
| title_fullStr | Effect of Cell Generations on t-BHP-induced Oxidative Stress Model of Caco-2 Cells |
| title_full_unstemmed | Effect of Cell Generations on t-BHP-induced Oxidative Stress Model of Caco-2 Cells |
| title_short | Effect of Cell Generations on t-BHP-induced Oxidative Stress Model of Caco-2 Cells |
| title_sort | effect of cell generations on t bhp induced oxidative stress model of caco 2 cells |
| topic | caco-2 cells tert-butyl hydrogen peroxide oxidative stress cell model cell passage number 8-hydroxydeoxyguanosine |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022050222 |
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