PlMYB308 Regulates Flower Senescence by Modulating Ethylene Biosynthesis in Herbaceous Peony
Herbaceous peony is an important cut-flower plant cultivated worldwide, but its short vase life substantially restricts its economic value. It is well established that endogenous hormones regulate the senescence process, but their molecular mechanism in flower senescence remains unclear. Here, we is...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-05-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.872442/full |
| _version_ | 1818243063976296448 |
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| author | Xiaotong Ji Meiling Wang Zhuangzhuang Xu Kai Wang Daoyang Sun Lixin Niu |
| author_facet | Xiaotong Ji Meiling Wang Zhuangzhuang Xu Kai Wang Daoyang Sun Lixin Niu |
| author_sort | Xiaotong Ji |
| collection | DOAJ |
| description | Herbaceous peony is an important cut-flower plant cultivated worldwide, but its short vase life substantially restricts its economic value. It is well established that endogenous hormones regulate the senescence process, but their molecular mechanism in flower senescence remains unclear. Here, we isolated a MYB transcription factor gene, PlMYB308, from herbaceous peony flowers, based on transcriptome data. Quantitative real-time PCR analysis showed that PlMYB308 is strongly up-regulated in senescing petals, and its expression was induced by abscisic acid or ethylene and reduced by gibberellin in petals. Treatment with abscisic acid or ethylene accelerated herbaceous peony petal senescence, and gibberellin delayed the process. PlMYB308 silencing delayed peony flower senescence and dramatically increased gibberellin, but reduced ethylene and abscisic acid levels in petals. PlMYB308 ectopic overexpression in tobacco accelerated flower senescence and reduced gibberellin, but increased ethylene and abscisic acid accumulation. Correspondingly, five endogenous hormone biosynthetic genes showed variable expression levels in petals after PlMYB308 silencing or overexpression. A dual-luciferase assay and yeast one-hybrid analysis showed that PlMYB308 specifically binds the PlACO1 promoter. Moreover, treatment with ethylene and 1-MCP can accelerate PlMYB308 silencing-reduced senescence and delay PlMYB308- overexpression-induced senescence. We also found that PlACO1 silencing delayed senescence in herbaceous peony petals. Taken together, our results suggest that the PlMYB308-PlACO1 regulatory checkpoints positively mediate the production of ethylene, and thus contribute to senescence in herbaceous peony flowers. |
| first_indexed | 2024-12-12T13:55:11Z |
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| id | doaj.art-51c95bc77493446eb8ecc63f84a5b3fd |
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| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-12T13:55:11Z |
| publishDate | 2022-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-51c95bc77493446eb8ecc63f84a5b3fd2022-12-22T00:22:28ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-05-011310.3389/fpls.2022.872442872442PlMYB308 Regulates Flower Senescence by Modulating Ethylene Biosynthesis in Herbaceous PeonyXiaotong JiMeiling WangZhuangzhuang XuKai WangDaoyang SunLixin NiuHerbaceous peony is an important cut-flower plant cultivated worldwide, but its short vase life substantially restricts its economic value. It is well established that endogenous hormones regulate the senescence process, but their molecular mechanism in flower senescence remains unclear. Here, we isolated a MYB transcription factor gene, PlMYB308, from herbaceous peony flowers, based on transcriptome data. Quantitative real-time PCR analysis showed that PlMYB308 is strongly up-regulated in senescing petals, and its expression was induced by abscisic acid or ethylene and reduced by gibberellin in petals. Treatment with abscisic acid or ethylene accelerated herbaceous peony petal senescence, and gibberellin delayed the process. PlMYB308 silencing delayed peony flower senescence and dramatically increased gibberellin, but reduced ethylene and abscisic acid levels in petals. PlMYB308 ectopic overexpression in tobacco accelerated flower senescence and reduced gibberellin, but increased ethylene and abscisic acid accumulation. Correspondingly, five endogenous hormone biosynthetic genes showed variable expression levels in petals after PlMYB308 silencing or overexpression. A dual-luciferase assay and yeast one-hybrid analysis showed that PlMYB308 specifically binds the PlACO1 promoter. Moreover, treatment with ethylene and 1-MCP can accelerate PlMYB308 silencing-reduced senescence and delay PlMYB308- overexpression-induced senescence. We also found that PlACO1 silencing delayed senescence in herbaceous peony petals. Taken together, our results suggest that the PlMYB308-PlACO1 regulatory checkpoints positively mediate the production of ethylene, and thus contribute to senescence in herbaceous peony flowers.https://www.frontiersin.org/articles/10.3389/fpls.2022.872442/fullherbaceous peonyflower senescencePlMYB308ethyleneplant hormones |
| spellingShingle | Xiaotong Ji Meiling Wang Zhuangzhuang Xu Kai Wang Daoyang Sun Lixin Niu PlMYB308 Regulates Flower Senescence by Modulating Ethylene Biosynthesis in Herbaceous Peony Frontiers in Plant Science herbaceous peony flower senescence PlMYB308 ethylene plant hormones |
| title | PlMYB308 Regulates Flower Senescence by Modulating Ethylene Biosynthesis in Herbaceous Peony |
| title_full | PlMYB308 Regulates Flower Senescence by Modulating Ethylene Biosynthesis in Herbaceous Peony |
| title_fullStr | PlMYB308 Regulates Flower Senescence by Modulating Ethylene Biosynthesis in Herbaceous Peony |
| title_full_unstemmed | PlMYB308 Regulates Flower Senescence by Modulating Ethylene Biosynthesis in Herbaceous Peony |
| title_short | PlMYB308 Regulates Flower Senescence by Modulating Ethylene Biosynthesis in Herbaceous Peony |
| title_sort | plmyb308 regulates flower senescence by modulating ethylene biosynthesis in herbaceous peony |
| topic | herbaceous peony flower senescence PlMYB308 ethylene plant hormones |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.872442/full |
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