Origin, prospective identification, and function of circulating endothelial colony-forming cells in mice and humans
Most circulating endothelial cells are apoptotic, but rare circulating endothelial colony-forming cells (C-ECFCs), also known as blood outgrowth endothelial cells, with proliferative and vasculogenic activity can be cultured; however, the origin and naive function of these C-ECFCs remains obscure. H...
| Main Authors: | , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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American Society for Clinical investigation
2023-03-01
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| Series: | JCI Insight |
| Subjects: | |
| Online Access: | https://doi.org/10.1172/jci.insight.164781 |
| _version_ | 1827768019332366336 |
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| author | Yang Lin Kimihiko Banno Chang-Hyun Gil Jered Myslinski Takashi Hato William C. Shelley Hongyu Gao Xiaoling Xuei Yunlong Liu David P. Basile Momoko Yoshimoto Nutan Prasain Stefan P. Tarnawsky Ralf H. Adams Katsuhiko Naruse Junko Yoshida Michael P. Murphy Kyoji Horie Mervin C. Yoder |
| author_facet | Yang Lin Kimihiko Banno Chang-Hyun Gil Jered Myslinski Takashi Hato William C. Shelley Hongyu Gao Xiaoling Xuei Yunlong Liu David P. Basile Momoko Yoshimoto Nutan Prasain Stefan P. Tarnawsky Ralf H. Adams Katsuhiko Naruse Junko Yoshida Michael P. Murphy Kyoji Horie Mervin C. Yoder |
| author_sort | Yang Lin |
| collection | DOAJ |
| description | Most circulating endothelial cells are apoptotic, but rare circulating endothelial colony-forming cells (C-ECFCs), also known as blood outgrowth endothelial cells, with proliferative and vasculogenic activity can be cultured; however, the origin and naive function of these C-ECFCs remains obscure. Herein, detailed lineage tracing revealed murine C-ECFCs emerged in the early postnatal period, displayed high vasculogenic potential with enriched frequency of clonal proliferative cells compared with tissue-resident ECFCs, and were not committed to or derived from the BM hematopoietic system but from tissue-resident ECFCs. In humans, C-ECFCs were present in the CD34bright cord blood mononuclear subset, possessed proliferative potential and in vivo vasculogenic function in a naive or cultured state, and displayed a single cell transcriptome sharing some umbilical venous endothelial cell features, such as a higher protein C receptor and extracellular matrix gene expression. This study provides an advance for the field by identifying the origin, naive function, and antigens to prospectively isolate C-ECFCs for translational studies. |
| first_indexed | 2024-03-11T12:06:33Z |
| format | Article |
| id | doaj.art-51cd15f30c264e4c8c211f1f7ecdd59c |
| institution | Directory Open Access Journal |
| issn | 2379-3708 |
| language | English |
| last_indexed | 2024-03-11T12:06:33Z |
| publishDate | 2023-03-01 |
| publisher | American Society for Clinical investigation |
| record_format | Article |
| series | JCI Insight |
| spelling | doaj.art-51cd15f30c264e4c8c211f1f7ecdd59c2023-11-07T16:25:19ZengAmerican Society for Clinical investigationJCI Insight2379-37082023-03-0185Origin, prospective identification, and function of circulating endothelial colony-forming cells in mice and humansYang LinKimihiko BannoChang-Hyun GilJered MyslinskiTakashi HatoWilliam C. ShelleyHongyu GaoXiaoling XueiYunlong LiuDavid P. BasileMomoko YoshimotoNutan PrasainStefan P. TarnawskyRalf H. AdamsKatsuhiko NaruseJunko YoshidaMichael P. MurphyKyoji HorieMervin C. YoderMost circulating endothelial cells are apoptotic, but rare circulating endothelial colony-forming cells (C-ECFCs), also known as blood outgrowth endothelial cells, with proliferative and vasculogenic activity can be cultured; however, the origin and naive function of these C-ECFCs remains obscure. Herein, detailed lineage tracing revealed murine C-ECFCs emerged in the early postnatal period, displayed high vasculogenic potential with enriched frequency of clonal proliferative cells compared with tissue-resident ECFCs, and were not committed to or derived from the BM hematopoietic system but from tissue-resident ECFCs. In humans, C-ECFCs were present in the CD34bright cord blood mononuclear subset, possessed proliferative potential and in vivo vasculogenic function in a naive or cultured state, and displayed a single cell transcriptome sharing some umbilical venous endothelial cell features, such as a higher protein C receptor and extracellular matrix gene expression. This study provides an advance for the field by identifying the origin, naive function, and antigens to prospectively isolate C-ECFCs for translational studies.https://doi.org/10.1172/jci.insight.164781Vascular biology |
| spellingShingle | Yang Lin Kimihiko Banno Chang-Hyun Gil Jered Myslinski Takashi Hato William C. Shelley Hongyu Gao Xiaoling Xuei Yunlong Liu David P. Basile Momoko Yoshimoto Nutan Prasain Stefan P. Tarnawsky Ralf H. Adams Katsuhiko Naruse Junko Yoshida Michael P. Murphy Kyoji Horie Mervin C. Yoder Origin, prospective identification, and function of circulating endothelial colony-forming cells in mice and humans JCI Insight Vascular biology |
| title | Origin, prospective identification, and function of circulating endothelial colony-forming cells in mice and humans |
| title_full | Origin, prospective identification, and function of circulating endothelial colony-forming cells in mice and humans |
| title_fullStr | Origin, prospective identification, and function of circulating endothelial colony-forming cells in mice and humans |
| title_full_unstemmed | Origin, prospective identification, and function of circulating endothelial colony-forming cells in mice and humans |
| title_short | Origin, prospective identification, and function of circulating endothelial colony-forming cells in mice and humans |
| title_sort | origin prospective identification and function of circulating endothelial colony forming cells in mice and humans |
| topic | Vascular biology |
| url | https://doi.org/10.1172/jci.insight.164781 |
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