Comparison of virulence factors between ESBL and non-ESBL producing Klebsiella pneumoniae isolates
Background and Objectives: Klebsiella pneumoniae is an opportunistic pathogen responsible for causing nosocomial and community-acquired infections. Its pathogenicity is associated with a variety of virulence factors and antibiotic resistance. The aim of the present study was to compare virulence at...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Tehran University of Medical Sciences
2024-02-01
|
| Series: | Iranian Journal of Microbiology |
| Subjects: | |
| Online Access: | https://ijm.tums.ac.ir/index.php/ijm/article/view/4428 |
| _version_ | 1797303924533755904 |
|---|---|
| author | Hamed Hatami Mirbag Saeed Tajbakhsh Azam Askari Forough Yousefi |
| author_facet | Hamed Hatami Mirbag Saeed Tajbakhsh Azam Askari Forough Yousefi |
| author_sort | Hamed Hatami Mirbag |
| collection | DOAJ |
| description |
Background and Objectives: Klebsiella pneumoniae is an opportunistic pathogen responsible for causing nosocomial and community-acquired infections. Its pathogenicity is associated with a variety of virulence factors and antibiotic resistance. The aim of the present study was to compare virulence attributes between ESBL and non-ESBL producing isolates.
Materials and Methods: A total of 113 K. pneumoniae including 56 ESBL and 57 non ESBL-producers were collected in Bushehr province, Iran, from November 2017 to February 2019. Enzymatic profile, hypermucoviscosity and biofilm formation were investigated phenotypically. In addition, the presence of rmpA, aerobactin, kfu, allS, mrkD, ybtS, entB, iutA, fimH, wabG, wcaG, K1 and K2 genes were detected by PCR and sequencing.
Results: There was no statistically significant difference in enzymatic profile between ESBL and non-ESBL producers. The prevalence of the hypermocoviscosity was lower among ESBL compared to non-ESBL producers but the intensity of biofilm was higher in the ESBL producers. Among the virulence genes, K1, rmpA, iutA, and aero were observed only in non-ESBLs. Moreover, the carriage of allS, K, K2, rmpA, iutA and aero genes was higher in hypermucoviscous in comparison with non hypermucoviscous isolates.
Conclusion: The identification of potentially pathogenic isolates plays an important role in preventing their spread as well as the success of their treatment.
|
| first_indexed | 2024-03-08T00:01:47Z |
| format | Article |
| id | doaj.art-51ce18f3b3724720a8b42e1a71980218 |
| institution | Directory Open Access Journal |
| issn | 2008-3289 2008-4447 |
| language | English |
| last_indexed | 2024-03-08T00:01:47Z |
| publishDate | 2024-02-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Microbiology |
| spelling | doaj.art-51ce18f3b3724720a8b42e1a719802182024-02-18T04:04:21ZengTehran University of Medical SciencesIranian Journal of Microbiology2008-32892008-44472024-02-0116110.18502/ijm.v16i1.14869Comparison of virulence factors between ESBL and non-ESBL producing Klebsiella pneumoniae isolatesHamed Hatami Mirbag0Saeed Tajbakhsh1Azam Askari2Forough Yousefi3Department of Microbiology and Parasitology, School of Medicine, Bushehr University of Medical Sciences, Bushehr, Iran; Student Research Committee, Bushehr University of Medical Sciences, Bushehr, IranDepartment of Microbiology and Parasitology, School of Medicine, Bushehr University of Medical Sciences, Bushehr, Iran; The Persian Gulf Marine Biotechnology Research Center, The Persian Gulf Biomedical Sciences, Research Institute, Bushehr University of Medical Sciences, Bushehr, IranDepartment of Microbiology and Parasitology, School of Medicine, Bushehr University of Medical Sciences, Bushehr, IranDepartment of Microbiology and Parasitology, School of Medicine, Bushehr University of Medical Sciences, Bushehr, Iran; The Persian Gulf Marine Biotechnology Research Center, The Persian Gulf Biomedical Sciences, Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran Background and Objectives: Klebsiella pneumoniae is an opportunistic pathogen responsible for causing nosocomial and community-acquired infections. Its pathogenicity is associated with a variety of virulence factors and antibiotic resistance. The aim of the present study was to compare virulence attributes between ESBL and non-ESBL producing isolates. Materials and Methods: A total of 113 K. pneumoniae including 56 ESBL and 57 non ESBL-producers were collected in Bushehr province, Iran, from November 2017 to February 2019. Enzymatic profile, hypermucoviscosity and biofilm formation were investigated phenotypically. In addition, the presence of rmpA, aerobactin, kfu, allS, mrkD, ybtS, entB, iutA, fimH, wabG, wcaG, K1 and K2 genes were detected by PCR and sequencing. Results: There was no statistically significant difference in enzymatic profile between ESBL and non-ESBL producers. The prevalence of the hypermocoviscosity was lower among ESBL compared to non-ESBL producers but the intensity of biofilm was higher in the ESBL producers. Among the virulence genes, K1, rmpA, iutA, and aero were observed only in non-ESBLs. Moreover, the carriage of allS, K, K2, rmpA, iutA and aero genes was higher in hypermucoviscous in comparison with non hypermucoviscous isolates. Conclusion: The identification of potentially pathogenic isolates plays an important role in preventing their spread as well as the success of their treatment. https://ijm.tums.ac.ir/index.php/ijm/article/view/4428Klebsiella pneumoniae;Biofilm;Extended-spectrum beta-lactamase;Virulence |
| spellingShingle | Hamed Hatami Mirbag Saeed Tajbakhsh Azam Askari Forough Yousefi Comparison of virulence factors between ESBL and non-ESBL producing Klebsiella pneumoniae isolates Iranian Journal of Microbiology Klebsiella pneumoniae; Biofilm; Extended-spectrum beta-lactamase; Virulence |
| title | Comparison of virulence factors between ESBL and non-ESBL producing Klebsiella pneumoniae isolates |
| title_full | Comparison of virulence factors between ESBL and non-ESBL producing Klebsiella pneumoniae isolates |
| title_fullStr | Comparison of virulence factors between ESBL and non-ESBL producing Klebsiella pneumoniae isolates |
| title_full_unstemmed | Comparison of virulence factors between ESBL and non-ESBL producing Klebsiella pneumoniae isolates |
| title_short | Comparison of virulence factors between ESBL and non-ESBL producing Klebsiella pneumoniae isolates |
| title_sort | comparison of virulence factors between esbl and non esbl producing klebsiella pneumoniae isolates |
| topic | Klebsiella pneumoniae; Biofilm; Extended-spectrum beta-lactamase; Virulence |
| url | https://ijm.tums.ac.ir/index.php/ijm/article/view/4428 |
| work_keys_str_mv | AT hamedhatamimirbag comparisonofvirulencefactorsbetweenesblandnonesblproducingklebsiellapneumoniaeisolates AT saeedtajbakhsh comparisonofvirulencefactorsbetweenesblandnonesblproducingklebsiellapneumoniaeisolates AT azamaskari comparisonofvirulencefactorsbetweenesblandnonesblproducingklebsiellapneumoniaeisolates AT foroughyousefi comparisonofvirulencefactorsbetweenesblandnonesblproducingklebsiellapneumoniaeisolates |