A Novel Prototype Biosensor Array Electrode System for Detecting the Bacterial Pathogen <i>Salmonella typhimurium</i>
Salmonellosis caused by <i>Salmonella</i> sp. has long been reported all over the world. Despite the availability of various diagnostic methods, easy and effective detection systems are still required. This report describes a dialysis membrane electrode interface disc with immobilized sp...
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| Format: | Article |
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MDPI AG
2022-06-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/12/6/389 |
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| author | Palaniappan Ramasamy Gajalakshmi Dakshinamoorthy Shanmugam Jayashree Dhamodharan Prabhu Sundararaj Rajamanikandan Palaniyandi Velusamy Govindan Dayanithi Robert E. B. Hanna |
| author_facet | Palaniappan Ramasamy Gajalakshmi Dakshinamoorthy Shanmugam Jayashree Dhamodharan Prabhu Sundararaj Rajamanikandan Palaniyandi Velusamy Govindan Dayanithi Robert E. B. Hanna |
| author_sort | Palaniappan Ramasamy |
| collection | DOAJ |
| description | Salmonellosis caused by <i>Salmonella</i> sp. has long been reported all over the world. Despite the availability of various diagnostic methods, easy and effective detection systems are still required. This report describes a dialysis membrane electrode interface disc with immobilized specific antibodies to capture antigenic <i>Salmonella</i> cells. The interaction of a specific <i>Salmonella</i> antigen with a mouse anti-<i>Salmonella</i> monoclonal antibody complexed to rabbit anti-mouse secondary antibody conjugated with HRP and the substrate o-aminophenol resulted in a response signal output current measured using two electrode systems (cadmium reference electrode and glassy carbon working electrode) and an agilent HP34401A 6.5 digital multimeter without a potentiostat or applied potential input. A maximum response signal output current was recorded for various concentrations of <i>Salmonella</i> viz., 3, 30, 300, 3000, 30,000 and 300,000 cells. The biosensor has a detection limit of three cells, which is very sensitive when compared with other detection sensors. Little non-specific response was observed using <i>Streptococcus</i>, <i>Vibrio</i>, and <i>Pseudomonas</i> sp. The maximum response signal output current for a dialysis membrane electrode interface disc was greater than that for gelatin, collagen, and agarose. The device and technique have a range of biological applications. This novel detection system has great potential for future development and application in surveillance for microbial pathogens. |
| first_indexed | 2024-03-10T00:17:51Z |
| format | Article |
| id | doaj.art-51cf747a41174abaaa92e94eaa29e2ef |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-10T00:17:51Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-51cf747a41174abaaa92e94eaa29e2ef2023-11-23T15:48:48ZengMDPI AGBiosensors2079-63742022-06-0112638910.3390/bios12060389A Novel Prototype Biosensor Array Electrode System for Detecting the Bacterial Pathogen <i>Salmonella typhimurium</i>Palaniappan Ramasamy0Gajalakshmi Dakshinamoorthy1Shanmugam Jayashree2Dhamodharan Prabhu3Sundararaj Rajamanikandan4Palaniyandi Velusamy5Govindan Dayanithi6Robert E. B. Hanna7Research and Development Wing, Bharath Institute of Higher Education and Research (BIHER), Sree Balaji Medical College and Hospital (SBMCH), Chromepet, Chennai 600044, Tamil Nadu, IndiaDepartment of Biotechnology, University of Madras, Chennai 600025, Tamil Nadu, IndiaDepartment of Biotechnology, University of Madras, Chennai 600025, Tamil Nadu, IndiaResearch and Development Wing, Bharath Institute of Higher Education and Research (BIHER), Sree Balaji Medical College and Hospital (SBMCH), Chromepet, Chennai 600044, Tamil Nadu, IndiaResearch and Development Wing, Bharath Institute of Higher Education and Research (BIHER), Sree Balaji Medical College and Hospital (SBMCH), Chromepet, Chennai 600044, Tamil Nadu, IndiaResearch and Development Wing, Bharath Institute of Higher Education and Research (BIHER), Sree Balaji Medical College and Hospital (SBMCH), Chromepet, Chennai 600044, Tamil Nadu, IndiaResearch and Development Wing, Bharath Institute of Higher Education and Research (BIHER), Sree Balaji Medical College and Hospital (SBMCH), Chromepet, Chennai 600044, Tamil Nadu, IndiaSchool of Biology and Biochemistry, The Queen’s University of Belfast, Belfast BT7 1NN, UKSalmonellosis caused by <i>Salmonella</i> sp. has long been reported all over the world. Despite the availability of various diagnostic methods, easy and effective detection systems are still required. This report describes a dialysis membrane electrode interface disc with immobilized specific antibodies to capture antigenic <i>Salmonella</i> cells. The interaction of a specific <i>Salmonella</i> antigen with a mouse anti-<i>Salmonella</i> monoclonal antibody complexed to rabbit anti-mouse secondary antibody conjugated with HRP and the substrate o-aminophenol resulted in a response signal output current measured using two electrode systems (cadmium reference electrode and glassy carbon working electrode) and an agilent HP34401A 6.5 digital multimeter without a potentiostat or applied potential input. A maximum response signal output current was recorded for various concentrations of <i>Salmonella</i> viz., 3, 30, 300, 3000, 30,000 and 300,000 cells. The biosensor has a detection limit of three cells, which is very sensitive when compared with other detection sensors. Little non-specific response was observed using <i>Streptococcus</i>, <i>Vibrio</i>, and <i>Pseudomonas</i> sp. The maximum response signal output current for a dialysis membrane electrode interface disc was greater than that for gelatin, collagen, and agarose. The device and technique have a range of biological applications. This novel detection system has great potential for future development and application in surveillance for microbial pathogens.https://www.mdpi.com/2079-6374/12/6/389<i>Salmonella</i> speciesbiosensorimmunosensordialysis membrane electrode interface discglassy carbon electrodesalmonellosis |
| spellingShingle | Palaniappan Ramasamy Gajalakshmi Dakshinamoorthy Shanmugam Jayashree Dhamodharan Prabhu Sundararaj Rajamanikandan Palaniyandi Velusamy Govindan Dayanithi Robert E. B. Hanna A Novel Prototype Biosensor Array Electrode System for Detecting the Bacterial Pathogen <i>Salmonella typhimurium</i> Biosensors <i>Salmonella</i> species biosensor immunosensor dialysis membrane electrode interface disc glassy carbon electrode salmonellosis |
| title | A Novel Prototype Biosensor Array Electrode System for Detecting the Bacterial Pathogen <i>Salmonella typhimurium</i> |
| title_full | A Novel Prototype Biosensor Array Electrode System for Detecting the Bacterial Pathogen <i>Salmonella typhimurium</i> |
| title_fullStr | A Novel Prototype Biosensor Array Electrode System for Detecting the Bacterial Pathogen <i>Salmonella typhimurium</i> |
| title_full_unstemmed | A Novel Prototype Biosensor Array Electrode System for Detecting the Bacterial Pathogen <i>Salmonella typhimurium</i> |
| title_short | A Novel Prototype Biosensor Array Electrode System for Detecting the Bacterial Pathogen <i>Salmonella typhimurium</i> |
| title_sort | novel prototype biosensor array electrode system for detecting the bacterial pathogen i salmonella typhimurium i |
| topic | <i>Salmonella</i> species biosensor immunosensor dialysis membrane electrode interface disc glassy carbon electrode salmonellosis |
| url | https://www.mdpi.com/2079-6374/12/6/389 |
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