Extensive Analysis of miRNA Trimming and Tailing Indicates that AGO1 Has a Complex Role in miRNA Turnover
MicroRNAs are small regulatory RNAs involved in several processes in plants ranging from development and stress responses to defense against pathogens. In order to accomplish their molecular functions, miRNAs are methylated and loaded into one ARGONAUTE (AGO) protein, commonly known as AGO1, to stab...
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MDPI AG
2021-01-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/10/2/267 |
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| author | Axel J. Giudicatti Ariel H. Tomassi Pablo A. Manavella Agustin L. Arce |
| author_facet | Axel J. Giudicatti Ariel H. Tomassi Pablo A. Manavella Agustin L. Arce |
| author_sort | Axel J. Giudicatti |
| collection | DOAJ |
| description | MicroRNAs are small regulatory RNAs involved in several processes in plants ranging from development and stress responses to defense against pathogens. In order to accomplish their molecular functions, miRNAs are methylated and loaded into one ARGONAUTE (AGO) protein, commonly known as AGO1, to stabilize and protect the molecule and to assemble a functional RNA-induced silencing complex (RISC). A specific machinery controls miRNA turnover to ensure the silencing release of targeted-genes in given circumstances. The trimming and tailing of miRNAs are fundamental modifications related to their turnover and, hence, to their action. In order to gain a better understanding of these modifications, we analyzed <i>Arabidopsis thaliana</i> small RNA sequencing data from a diversity of mutants, related to miRNA biogenesis, action, and turnover, and from different cellular fractions and immunoprecipitations. Besides confirming the effects of known players in these pathways, we found increased trimming and tailing in miRNA biogenesis mutants. More importantly, our analysis allowed us to reveal the importance of ARGONAUTE 1 (AGO1) loading, slicing activity, and cellular localization in trimming and tailing of miRNAs. |
| first_indexed | 2024-03-09T03:15:51Z |
| format | Article |
| id | doaj.art-51d13ce7b6db4e318d92bc0accef0b40 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-09T03:15:51Z |
| publishDate | 2021-01-01 |
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| series | Plants |
| spelling | doaj.art-51d13ce7b6db4e318d92bc0accef0b402023-12-03T15:20:24ZengMDPI AGPlants2223-77472021-01-0110226710.3390/plants10020267Extensive Analysis of miRNA Trimming and Tailing Indicates that AGO1 Has a Complex Role in miRNA TurnoverAxel J. Giudicatti0Ariel H. Tomassi1Pablo A. Manavella2Agustin L. Arce3Instituto de Agrobiotecnología del Litoral (CONICET-UNL), Cátedra de Biología Celular y Molecular, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, 3000 Santa Fe, ArgentinaInstituto de Agrobiotecnología del Litoral (CONICET-UNL), Cátedra de Biología Celular y Molecular, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, 3000 Santa Fe, ArgentinaInstituto de Agrobiotecnología del Litoral (CONICET-UNL), Cátedra de Biología Celular y Molecular, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, 3000 Santa Fe, ArgentinaInstituto de Agrobiotecnología del Litoral (CONICET-UNL), Cátedra de Biología Celular y Molecular, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, 3000 Santa Fe, ArgentinaMicroRNAs are small regulatory RNAs involved in several processes in plants ranging from development and stress responses to defense against pathogens. In order to accomplish their molecular functions, miRNAs are methylated and loaded into one ARGONAUTE (AGO) protein, commonly known as AGO1, to stabilize and protect the molecule and to assemble a functional RNA-induced silencing complex (RISC). A specific machinery controls miRNA turnover to ensure the silencing release of targeted-genes in given circumstances. The trimming and tailing of miRNAs are fundamental modifications related to their turnover and, hence, to their action. In order to gain a better understanding of these modifications, we analyzed <i>Arabidopsis thaliana</i> small RNA sequencing data from a diversity of mutants, related to miRNA biogenesis, action, and turnover, and from different cellular fractions and immunoprecipitations. Besides confirming the effects of known players in these pathways, we found increased trimming and tailing in miRNA biogenesis mutants. More importantly, our analysis allowed us to reveal the importance of ARGONAUTE 1 (AGO1) loading, slicing activity, and cellular localization in trimming and tailing of miRNAs.https://www.mdpi.com/2223-7747/10/2/267miRNA trimmingmiRNA tailingARGONAUTE 1miRNA turnover<i>Arabidopsis thaliana</i> |
| spellingShingle | Axel J. Giudicatti Ariel H. Tomassi Pablo A. Manavella Agustin L. Arce Extensive Analysis of miRNA Trimming and Tailing Indicates that AGO1 Has a Complex Role in miRNA Turnover Plants miRNA trimming miRNA tailing ARGONAUTE 1 miRNA turnover <i>Arabidopsis thaliana</i> |
| title | Extensive Analysis of miRNA Trimming and Tailing Indicates that AGO1 Has a Complex Role in miRNA Turnover |
| title_full | Extensive Analysis of miRNA Trimming and Tailing Indicates that AGO1 Has a Complex Role in miRNA Turnover |
| title_fullStr | Extensive Analysis of miRNA Trimming and Tailing Indicates that AGO1 Has a Complex Role in miRNA Turnover |
| title_full_unstemmed | Extensive Analysis of miRNA Trimming and Tailing Indicates that AGO1 Has a Complex Role in miRNA Turnover |
| title_short | Extensive Analysis of miRNA Trimming and Tailing Indicates that AGO1 Has a Complex Role in miRNA Turnover |
| title_sort | extensive analysis of mirna trimming and tailing indicates that ago1 has a complex role in mirna turnover |
| topic | miRNA trimming miRNA tailing ARGONAUTE 1 miRNA turnover <i>Arabidopsis thaliana</i> |
| url | https://www.mdpi.com/2223-7747/10/2/267 |
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