Customized antimicrobial efficacy tests offer superior evaluation of growth inhibitor efficacy for liquid microbial products
Antimicrobial effectiveness tests are common methods used to assess the risk of microbial contamination in pharmaceuticals and cosmetics. These assays may be inappropriate for endospore-based microbial products which often carry a similar – if not greater – risk of microbial contamination. In the pr...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Elsevier
2020-02-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844020302644 |
| _version_ | 1818127811480649728 |
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| author | John P. Gorsuch Zachary Jones |
| author_facet | John P. Gorsuch Zachary Jones |
| author_sort | John P. Gorsuch |
| collection | DOAJ |
| description | Antimicrobial effectiveness tests are common methods used to assess the risk of microbial contamination in pharmaceuticals and cosmetics. These assays may be inappropriate for endospore-based microbial products which often carry a similar – if not greater – risk of microbial contamination. In the present study, we compared the antimicrobial efficacy assessment provided by United States Pharmacopeia Chapter <51> Antimicrobial Effectiveness Testing with a modified test which utilized a customized bacterial challenge. The customized challenge inoculum comprised an assemblage of 12 bacterial strains (both pathogens and spoilage organisms) isolated from the product's end-use geography. Results suggest that some microbial inhibitor systems which pass industry standard antimicrobial effectiveness tests may fail when challenged with a customized bacterial assemblage. In order to provide the best possible assessment of microbial inhibitor systems for liquid Bacillus products, we suggest that new antimicrobial effectiveness tests be developed for this product class which include the addition of field-relevant contaminants in addition to the industry standard pathogen challenge. |
| first_indexed | 2024-12-11T07:23:17Z |
| format | Article |
| id | doaj.art-51d577a2f8ee4ce6bbb4e45730ee412b |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-12-11T07:23:17Z |
| publishDate | 2020-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-51d577a2f8ee4ce6bbb4e45730ee412b2022-12-22T01:16:02ZengElsevierHeliyon2405-84402020-02-0162e03419Customized antimicrobial efficacy tests offer superior evaluation of growth inhibitor efficacy for liquid microbial productsJohn P. Gorsuch0Zachary Jones1Corresponding author.; BiOWiSH Technologies, 2724 Erie Avenue Suite C, Cincinnati, OH, 45208, USABiOWiSH Technologies, 2724 Erie Avenue Suite C, Cincinnati, OH, 45208, USAAntimicrobial effectiveness tests are common methods used to assess the risk of microbial contamination in pharmaceuticals and cosmetics. These assays may be inappropriate for endospore-based microbial products which often carry a similar – if not greater – risk of microbial contamination. In the present study, we compared the antimicrobial efficacy assessment provided by United States Pharmacopeia Chapter <51> Antimicrobial Effectiveness Testing with a modified test which utilized a customized bacterial challenge. The customized challenge inoculum comprised an assemblage of 12 bacterial strains (both pathogens and spoilage organisms) isolated from the product's end-use geography. Results suggest that some microbial inhibitor systems which pass industry standard antimicrobial effectiveness tests may fail when challenged with a customized bacterial assemblage. In order to provide the best possible assessment of microbial inhibitor systems for liquid Bacillus products, we suggest that new antimicrobial effectiveness tests be developed for this product class which include the addition of field-relevant contaminants in addition to the industry standard pathogen challenge.http://www.sciencedirect.com/science/article/pii/S2405844020302644MicrobiologyAntimicrobial effectiveness testBacillusLiquid microbial productsBacterial contamination |
| spellingShingle | John P. Gorsuch Zachary Jones Customized antimicrobial efficacy tests offer superior evaluation of growth inhibitor efficacy for liquid microbial products Heliyon Microbiology Antimicrobial effectiveness test Bacillus Liquid microbial products Bacterial contamination |
| title | Customized antimicrobial efficacy tests offer superior evaluation of growth inhibitor efficacy for liquid microbial products |
| title_full | Customized antimicrobial efficacy tests offer superior evaluation of growth inhibitor efficacy for liquid microbial products |
| title_fullStr | Customized antimicrobial efficacy tests offer superior evaluation of growth inhibitor efficacy for liquid microbial products |
| title_full_unstemmed | Customized antimicrobial efficacy tests offer superior evaluation of growth inhibitor efficacy for liquid microbial products |
| title_short | Customized antimicrobial efficacy tests offer superior evaluation of growth inhibitor efficacy for liquid microbial products |
| title_sort | customized antimicrobial efficacy tests offer superior evaluation of growth inhibitor efficacy for liquid microbial products |
| topic | Microbiology Antimicrobial effectiveness test Bacillus Liquid microbial products Bacterial contamination |
| url | http://www.sciencedirect.com/science/article/pii/S2405844020302644 |
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