Navigating the microbial community in the trachea-oropharynx of breast cancer patients with or without neoadjuvant chemotherapy (NAC) via endotracheal tube: has NAC caused any change?
Background We compare the diversity and niche specificity of the microbiome in the trachea-oropharynx microbiome of malignant breast neoplasm with or without neoadjuvant chemotherapy (NAC) via NGS analysis. Methods We prospectively collected a total of 40 endotracheal tubes intubated from subjects,...
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PeerJ Inc.
2023-11-01
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| Online Access: | https://peerj.com/articles/16366.pdf |
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| author | Hee Yeon Kim Tae Hyun Kim Jeong Hwan Shin Kwangrae Cho Heon-Kyun Ha Anbok Lee Young Jin Kim |
| author_facet | Hee Yeon Kim Tae Hyun Kim Jeong Hwan Shin Kwangrae Cho Heon-Kyun Ha Anbok Lee Young Jin Kim |
| author_sort | Hee Yeon Kim |
| collection | DOAJ |
| description | Background We compare the diversity and niche specificity of the microbiome in the trachea-oropharynx microbiome of malignant breast neoplasm with or without neoadjuvant chemotherapy (NAC) via NGS analysis. Methods We prospectively collected a total of 40 endotracheal tubes intubated from subjects, of whom 20 with NAC treated breast cancer (NAC group) and 20 with breast cancer without NAC (Non-NAC group). We generated 16S rRNA-based microbial profiles in IlluminaTM platform and alpha diversity indices were compared between groups. For the comparison of taxa abundance, linear discriminant analysis effect size method with Kruskal-Wallis test was used. The distribution of variables between the two groups was compared using the Mann-Whitney test. For beta diversity analysis, PERMANOVA was used. Results Among the diversity indices, the NAC group showed significantly lower Chao1, Inverse Simpson, and Shannon indices than the Non-NAC group. The three most frequent taxa of all two groups were Streptococcus (20.4%), followed by Veillonella (11.9%), and Prevorella (10.4%). This order was the same in NAC and non-NAC groups. Conclusion Here, we provide the first comparison data of the respiratory tract microbiome of breast cancer patients with or without NAC via NGS analysis. This study ultimately seeks to contribute to future studies on the lower respiratory tract in cancer patients with cytotoxic chemotherapy by establishing reliable control data. |
| first_indexed | 2024-03-09T15:37:53Z |
| format | Article |
| id | doaj.art-51d57a64e4104b04aeeaacb4b14bd09f |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T15:37:53Z |
| publishDate | 2023-11-01 |
| publisher | PeerJ Inc. |
| record_format | Article |
| series | PeerJ |
| spelling | doaj.art-51d57a64e4104b04aeeaacb4b14bd09f2023-11-25T15:05:05ZengPeerJ Inc.PeerJ2167-83592023-11-0111e1636610.7717/peerj.16366Navigating the microbial community in the trachea-oropharynx of breast cancer patients with or without neoadjuvant chemotherapy (NAC) via endotracheal tube: has NAC caused any change?Hee Yeon Kim0Tae Hyun Kim1Jeong Hwan Shin2Kwangrae Cho3Heon-Kyun Ha4Anbok Lee5Young Jin Kim6Department of Surgery, Busan Paik Hospital, Inje University, Busan, South KoreaDepartment of Surgery, Busan Paik Hospital, Inje University, Busan, South KoreaDepartment of Laboratory Medicine and Paik Institute for Clinical Research, Inje University, Busan, South KoreaDepartment of Anesthesiology and Pain Medicine, Busan Paik Hospital, Inje University, Busan, South KoreaDepartment of Surgery, Chung-Ang University Gwangmyeong Hospital, Chung-Ang University College of Medicine, Chung-Ang University, Gyeonggi-do, South KoreaDepartment of Surgery, Chung-Ang University Gwangmyeong Hospital, Chung-Ang University College of Medicine, Chung-Ang University, Gyeonggi-do, South KoreaDepartment of Laboratory Medicine, Kyung Hee University College of Medicine, Kyung Hee University Medical Center, Seoul, South KoreaBackground We compare the diversity and niche specificity of the microbiome in the trachea-oropharynx microbiome of malignant breast neoplasm with or without neoadjuvant chemotherapy (NAC) via NGS analysis. Methods We prospectively collected a total of 40 endotracheal tubes intubated from subjects, of whom 20 with NAC treated breast cancer (NAC group) and 20 with breast cancer without NAC (Non-NAC group). We generated 16S rRNA-based microbial profiles in IlluminaTM platform and alpha diversity indices were compared between groups. For the comparison of taxa abundance, linear discriminant analysis effect size method with Kruskal-Wallis test was used. The distribution of variables between the two groups was compared using the Mann-Whitney test. For beta diversity analysis, PERMANOVA was used. Results Among the diversity indices, the NAC group showed significantly lower Chao1, Inverse Simpson, and Shannon indices than the Non-NAC group. The three most frequent taxa of all two groups were Streptococcus (20.4%), followed by Veillonella (11.9%), and Prevorella (10.4%). This order was the same in NAC and non-NAC groups. Conclusion Here, we provide the first comparison data of the respiratory tract microbiome of breast cancer patients with or without NAC via NGS analysis. This study ultimately seeks to contribute to future studies on the lower respiratory tract in cancer patients with cytotoxic chemotherapy by establishing reliable control data.https://peerj.com/articles/16366.pdfBreast cancerLower respiratory tractMicrobiomeNeoadjuvant chemotherapy |
| spellingShingle | Hee Yeon Kim Tae Hyun Kim Jeong Hwan Shin Kwangrae Cho Heon-Kyun Ha Anbok Lee Young Jin Kim Navigating the microbial community in the trachea-oropharynx of breast cancer patients with or without neoadjuvant chemotherapy (NAC) via endotracheal tube: has NAC caused any change? PeerJ Breast cancer Lower respiratory tract Microbiome Neoadjuvant chemotherapy |
| title | Navigating the microbial community in the trachea-oropharynx of breast cancer patients with or without neoadjuvant chemotherapy (NAC) via endotracheal tube: has NAC caused any change? |
| title_full | Navigating the microbial community in the trachea-oropharynx of breast cancer patients with or without neoadjuvant chemotherapy (NAC) via endotracheal tube: has NAC caused any change? |
| title_fullStr | Navigating the microbial community in the trachea-oropharynx of breast cancer patients with or without neoadjuvant chemotherapy (NAC) via endotracheal tube: has NAC caused any change? |
| title_full_unstemmed | Navigating the microbial community in the trachea-oropharynx of breast cancer patients with or without neoadjuvant chemotherapy (NAC) via endotracheal tube: has NAC caused any change? |
| title_short | Navigating the microbial community in the trachea-oropharynx of breast cancer patients with or without neoadjuvant chemotherapy (NAC) via endotracheal tube: has NAC caused any change? |
| title_sort | navigating the microbial community in the trachea oropharynx of breast cancer patients with or without neoadjuvant chemotherapy nac via endotracheal tube has nac caused any change |
| topic | Breast cancer Lower respiratory tract Microbiome Neoadjuvant chemotherapy |
| url | https://peerj.com/articles/16366.pdf |
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