Ameliorative Effects of Peptides from the Oyster (<i>Crassostrea hongkongensis</i>) Protein Hydrolysates against UVB-Induced Skin Photodamage in Mice
Chronic exposure to ultraviolet B (UVB) irradiation is a major cause for skin photoaging. UVB induces damage to skin mainly by oxidative stress, inflammation, and collagen degradation. This paper investigated the photo-protective effects of peptides from oyster (<i>Crassostrea hongkongensis<...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-05-01
|
| Series: | Marine Drugs |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1660-3397/18/6/288 |
| _version_ | 1797566493025632256 |
|---|---|
| author | Zhilan Peng Beibei Chen Qinsheng Zheng Guoping Zhu Wenhong Cao Xiaoming Qin Chaohua Zhang |
| author_facet | Zhilan Peng Beibei Chen Qinsheng Zheng Guoping Zhu Wenhong Cao Xiaoming Qin Chaohua Zhang |
| author_sort | Zhilan Peng |
| collection | DOAJ |
| description | Chronic exposure to ultraviolet B (UVB) irradiation is a major cause for skin photoaging. UVB induces damage to skin mainly by oxidative stress, inflammation, and collagen degradation. This paper investigated the photo-protective effects of peptides from oyster (<i>Crassostrea hongkongensis</i>) protein hydrolysates (OPs) by topical application on the skin of UVB-irradiated mice. Results from mass spectrometry showed that OPs consisted of peptides with a molecular weight range of 302.17–2936.43 Da. In vivo study demonstrated that topical application of OPs on the skin significantly alleviated moisture loss, epidermal hyperplasia, as well as degradation of collagen and elastin fibers caused by chronic UVB irradiation. In this study, OPs treatment promoted antioxidant enzymes (SOD and GPH-Px) activities, while decreased malondialdehyde (MDA) level in the skin. In addition, OPs treatment significantly decreased inflammatory cytokines (IL-1β, IL-6, TNF-α) content, and inhibited inflammation related (iNOS, COX-2) protein expression in the skin. Via inhibiting metalloproteinase 1(MMP1) expression, OPs treatment markedly decreased the degradation of collagen and elastin fibers as well as recovered the altered arrangement of extracellular matrix network in the dermis of skin. Our study demonstrated for the first time that OPs protected against UVB induced skin photodamage by virtue of its antioxidative and anti-inflammatory properties, as well as regulating the abnormal expression of MMP-1. The possible molecular mechanism underlying OPs anti-photoaging is possibly related to downregulating of the MAPK/NF-κB signaling pathway, while promoting TGF-β production in the skin. |
| first_indexed | 2024-03-10T19:28:36Z |
| format | Article |
| id | doaj.art-51dc0333973e4095bf6950514c911b73 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-03-10T19:28:36Z |
| publishDate | 2020-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-51dc0333973e4095bf6950514c911b732023-11-20T02:20:56ZengMDPI AGMarine Drugs1660-33972020-05-0118628810.3390/md18060288Ameliorative Effects of Peptides from the Oyster (<i>Crassostrea hongkongensis</i>) Protein Hydrolysates against UVB-Induced Skin Photodamage in MiceZhilan Peng0Beibei Chen1Qinsheng Zheng2Guoping Zhu3Wenhong Cao4Xiaoming Qin5Chaohua Zhang6College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, ChinaCollege of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, ChinaCollege of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, ChinaCollege of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, ChinaCollege of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, ChinaCollege of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, ChinaCollege of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, ChinaChronic exposure to ultraviolet B (UVB) irradiation is a major cause for skin photoaging. UVB induces damage to skin mainly by oxidative stress, inflammation, and collagen degradation. This paper investigated the photo-protective effects of peptides from oyster (<i>Crassostrea hongkongensis</i>) protein hydrolysates (OPs) by topical application on the skin of UVB-irradiated mice. Results from mass spectrometry showed that OPs consisted of peptides with a molecular weight range of 302.17–2936.43 Da. In vivo study demonstrated that topical application of OPs on the skin significantly alleviated moisture loss, epidermal hyperplasia, as well as degradation of collagen and elastin fibers caused by chronic UVB irradiation. In this study, OPs treatment promoted antioxidant enzymes (SOD and GPH-Px) activities, while decreased malondialdehyde (MDA) level in the skin. In addition, OPs treatment significantly decreased inflammatory cytokines (IL-1β, IL-6, TNF-α) content, and inhibited inflammation related (iNOS, COX-2) protein expression in the skin. Via inhibiting metalloproteinase 1(MMP1) expression, OPs treatment markedly decreased the degradation of collagen and elastin fibers as well as recovered the altered arrangement of extracellular matrix network in the dermis of skin. Our study demonstrated for the first time that OPs protected against UVB induced skin photodamage by virtue of its antioxidative and anti-inflammatory properties, as well as regulating the abnormal expression of MMP-1. The possible molecular mechanism underlying OPs anti-photoaging is possibly related to downregulating of the MAPK/NF-κB signaling pathway, while promoting TGF-β production in the skin.https://www.mdpi.com/1660-3397/18/6/288polypeptidesoysterphotoagingantioxidativeanti-inflammatory |
| spellingShingle | Zhilan Peng Beibei Chen Qinsheng Zheng Guoping Zhu Wenhong Cao Xiaoming Qin Chaohua Zhang Ameliorative Effects of Peptides from the Oyster (<i>Crassostrea hongkongensis</i>) Protein Hydrolysates against UVB-Induced Skin Photodamage in Mice Marine Drugs polypeptides oyster photoaging antioxidative anti-inflammatory |
| title | Ameliorative Effects of Peptides from the Oyster (<i>Crassostrea hongkongensis</i>) Protein Hydrolysates against UVB-Induced Skin Photodamage in Mice |
| title_full | Ameliorative Effects of Peptides from the Oyster (<i>Crassostrea hongkongensis</i>) Protein Hydrolysates against UVB-Induced Skin Photodamage in Mice |
| title_fullStr | Ameliorative Effects of Peptides from the Oyster (<i>Crassostrea hongkongensis</i>) Protein Hydrolysates against UVB-Induced Skin Photodamage in Mice |
| title_full_unstemmed | Ameliorative Effects of Peptides from the Oyster (<i>Crassostrea hongkongensis</i>) Protein Hydrolysates against UVB-Induced Skin Photodamage in Mice |
| title_short | Ameliorative Effects of Peptides from the Oyster (<i>Crassostrea hongkongensis</i>) Protein Hydrolysates against UVB-Induced Skin Photodamage in Mice |
| title_sort | ameliorative effects of peptides from the oyster i crassostrea hongkongensis i protein hydrolysates against uvb induced skin photodamage in mice |
| topic | polypeptides oyster photoaging antioxidative anti-inflammatory |
| url | https://www.mdpi.com/1660-3397/18/6/288 |
| work_keys_str_mv | AT zhilanpeng ameliorativeeffectsofpeptidesfromtheoystericrassostreahongkongensisiproteinhydrolysatesagainstuvbinducedskinphotodamageinmice AT beibeichen ameliorativeeffectsofpeptidesfromtheoystericrassostreahongkongensisiproteinhydrolysatesagainstuvbinducedskinphotodamageinmice AT qinshengzheng ameliorativeeffectsofpeptidesfromtheoystericrassostreahongkongensisiproteinhydrolysatesagainstuvbinducedskinphotodamageinmice AT guopingzhu ameliorativeeffectsofpeptidesfromtheoystericrassostreahongkongensisiproteinhydrolysatesagainstuvbinducedskinphotodamageinmice AT wenhongcao ameliorativeeffectsofpeptidesfromtheoystericrassostreahongkongensisiproteinhydrolysatesagainstuvbinducedskinphotodamageinmice AT xiaomingqin ameliorativeeffectsofpeptidesfromtheoystericrassostreahongkongensisiproteinhydrolysatesagainstuvbinducedskinphotodamageinmice AT chaohuazhang ameliorativeeffectsofpeptidesfromtheoystericrassostreahongkongensisiproteinhydrolysatesagainstuvbinducedskinphotodamageinmice |