A DnaA‐dependent riboswitch for transcription attenuation of the his operon
Abstract Transcription attenuation in response to the availability of a specific amino acid is believed to be controlled by alternative configurations of RNA secondary structures that lead to the arrest of translation or the release of the arrested ribosome from the leader mRNA molecule. In this stu...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2023-06-01
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| Series: | mLife |
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| Online Access: | https://doi.org/10.1002/mlf2.12075 |
| _version_ | 1797791655691026432 |
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| author | Yuan Yao Hongwei Sun Wurihan Gegeheng Gezi Kirsten Skarstad Lifei Fan Morigen |
| author_facet | Yuan Yao Hongwei Sun Wurihan Gegeheng Gezi Kirsten Skarstad Lifei Fan Morigen |
| author_sort | Yuan Yao |
| collection | DOAJ |
| description | Abstract Transcription attenuation in response to the availability of a specific amino acid is believed to be controlled by alternative configurations of RNA secondary structures that lead to the arrest of translation or the release of the arrested ribosome from the leader mRNA molecule. In this study, we first report a possible example of the DnaA‐dependent riboswitch for transcription attenuation in Escherichia coli. We show that (i) DnaA regulates the transcription of the structural genes but not that of the leader hisL gene; (ii) DnaA might bind to rDnaA boxes present in the HisL‐SL RNA, and subsequently attenuate the transcription of the operon; (iii) the HisL‐SL RNA and rDnaA boxes are phylogenetically conserved and evolutionarily important; and (iv) the translating ribosome is required for deattenuation of the his operon, whereas tRNAHis strengthens attenuation. This mechanism seems to be phylogenetically conserved in Gram‐negative bacteria and evolutionarily important. |
| first_indexed | 2024-03-13T02:21:56Z |
| format | Article |
| id | doaj.art-51e394801e3e47ee876ffac7fc704eb1 |
| institution | Directory Open Access Journal |
| issn | 2770-100X |
| language | English |
| last_indexed | 2024-03-13T02:21:56Z |
| publishDate | 2023-06-01 |
| publisher | Wiley |
| record_format | Article |
| series | mLife |
| spelling | doaj.art-51e394801e3e47ee876ffac7fc704eb12023-06-30T08:36:29ZengWileymLife2770-100X2023-06-012212614010.1002/mlf2.12075A DnaA‐dependent riboswitch for transcription attenuation of the his operonYuan Yao0Hongwei Sun1Wurihan2Gegeheng3Gezi4Kirsten Skarstad5Lifei Fan6Morigen7State Key Laboratory of Reproductive Regulation, Breeding of Grassland Livestock, School of Life Sciences Inner Mongolia University Hohhot ChinaState Key Laboratory of Reproductive Regulation, Breeding of Grassland Livestock, School of Life Sciences Inner Mongolia University Hohhot ChinaState Key Laboratory of Reproductive Regulation, Breeding of Grassland Livestock, School of Life Sciences Inner Mongolia University Hohhot ChinaState Key Laboratory of Reproductive Regulation, Breeding of Grassland Livestock, School of Life Sciences Inner Mongolia University Hohhot ChinaState Key Laboratory of Reproductive Regulation, Breeding of Grassland Livestock, School of Life Sciences Inner Mongolia University Hohhot ChinaDepartment of Molecular Cell Biology and Department of Microbiology Oslo University Hospital Oslo NorwayState Key Laboratory of Reproductive Regulation, Breeding of Grassland Livestock, School of Life Sciences Inner Mongolia University Hohhot ChinaState Key Laboratory of Reproductive Regulation, Breeding of Grassland Livestock, School of Life Sciences Inner Mongolia University Hohhot ChinaAbstract Transcription attenuation in response to the availability of a specific amino acid is believed to be controlled by alternative configurations of RNA secondary structures that lead to the arrest of translation or the release of the arrested ribosome from the leader mRNA molecule. In this study, we first report a possible example of the DnaA‐dependent riboswitch for transcription attenuation in Escherichia coli. We show that (i) DnaA regulates the transcription of the structural genes but not that of the leader hisL gene; (ii) DnaA might bind to rDnaA boxes present in the HisL‐SL RNA, and subsequently attenuate the transcription of the operon; (iii) the HisL‐SL RNA and rDnaA boxes are phylogenetically conserved and evolutionarily important; and (iv) the translating ribosome is required for deattenuation of the his operon, whereas tRNAHis strengthens attenuation. This mechanism seems to be phylogenetically conserved in Gram‐negative bacteria and evolutionarily important.https://doi.org/10.1002/mlf2.12075DnaA‐dependentEscherichia colihis operonriboswitchtranscription attenuation |
| spellingShingle | Yuan Yao Hongwei Sun Wurihan Gegeheng Gezi Kirsten Skarstad Lifei Fan Morigen A DnaA‐dependent riboswitch for transcription attenuation of the his operon mLife DnaA‐dependent Escherichia coli his operon riboswitch transcription attenuation |
| title | A DnaA‐dependent riboswitch for transcription attenuation of the his operon |
| title_full | A DnaA‐dependent riboswitch for transcription attenuation of the his operon |
| title_fullStr | A DnaA‐dependent riboswitch for transcription attenuation of the his operon |
| title_full_unstemmed | A DnaA‐dependent riboswitch for transcription attenuation of the his operon |
| title_short | A DnaA‐dependent riboswitch for transcription attenuation of the his operon |
| title_sort | dnaa dependent riboswitch for transcription attenuation of the his operon |
| topic | DnaA‐dependent Escherichia coli his operon riboswitch transcription attenuation |
| url | https://doi.org/10.1002/mlf2.12075 |
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