Differential immune responses of 3D gingival and periodontal connective tissue equivalents to microbial colonization
Gingival and periodontal ligament fibroblasts are functionally distinct cell types within the dento-gingival unit that participate in host immune response. Their microenvironment influences the behavior and immune response to microbial challenge. We developed three-dimensional gingival and periodont...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
SAGE Publishing
2022-07-01
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| Series: | Journal of Tissue Engineering |
| Online Access: | https://doi.org/10.1177/20417314221111650 |
| _version_ | 1811223310991622144 |
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| author | Hardik Makkar Srividya Atkuru Yi Ling Tang Tanya Sethi Chwee Teck Lim Kai Soo Tan Gopu Sriram |
| author_facet | Hardik Makkar Srividya Atkuru Yi Ling Tang Tanya Sethi Chwee Teck Lim Kai Soo Tan Gopu Sriram |
| author_sort | Hardik Makkar |
| collection | DOAJ |
| description | Gingival and periodontal ligament fibroblasts are functionally distinct cell types within the dento-gingival unit that participate in host immune response. Their microenvironment influences the behavior and immune response to microbial challenge. We developed three-dimensional gingival and periodontal connective tissue equivalents (CTEs) using human fibrin-based matrix. The CTEs were characterized, and the heterogeneity in their innate immune response was investigated. The CTEs demonstrated no to minimal response to planktonic Streptococcus mitis and Streptococcus oralis , while their biofilms elicited a moderate increase in IL-6 and IL-8 production. In contrast, Fusobacterium nucleatum provoked a substantial increase in IL-6 and IL-8 production. Interestingly, the gingival CTEs secreted significantly higher IL-6, while periodontal counterparts produced higher IL-8. In conclusion, the gingival and periodontal CTEs exhibited differential responses to various bacterial challenges. This gives insights into the contribution of tissue topography and fibroblast heterogeneity in rendering protective and specific immune responses toward early biofilm colonizers. |
| first_indexed | 2024-04-12T08:29:32Z |
| format | Article |
| id | doaj.art-51f07a0af0c649ca99be7719c2144cff |
| institution | Directory Open Access Journal |
| issn | 2041-7314 |
| language | English |
| last_indexed | 2024-04-12T08:29:32Z |
| publishDate | 2022-07-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Journal of Tissue Engineering |
| spelling | doaj.art-51f07a0af0c649ca99be7719c2144cff2022-12-22T03:40:14ZengSAGE PublishingJournal of Tissue Engineering2041-73142022-07-011310.1177/20417314221111650Differential immune responses of 3D gingival and periodontal connective tissue equivalents to microbial colonizationHardik Makkar0Srividya Atkuru1Yi Ling Tang2Tanya Sethi3Chwee Teck Lim4Kai Soo Tan5Gopu Sriram6Faculty of Dentistry, National University of Singapore, SingaporeFaculty of Dentistry, National University of Singapore, SingaporeFaculty of Dentistry, National University of Singapore, SingaporeFaculty of Dentistry, National University of Singapore, SingaporeInstitute for Health Innovation and Technology (iHealthtech), National University of Singapore, SingaporeFaculty of Dentistry, National University of Singapore, SingaporeFaculty of Dentistry, National University of Singapore, SingaporeGingival and periodontal ligament fibroblasts are functionally distinct cell types within the dento-gingival unit that participate in host immune response. Their microenvironment influences the behavior and immune response to microbial challenge. We developed three-dimensional gingival and periodontal connective tissue equivalents (CTEs) using human fibrin-based matrix. The CTEs were characterized, and the heterogeneity in their innate immune response was investigated. The CTEs demonstrated no to minimal response to planktonic Streptococcus mitis and Streptococcus oralis , while their biofilms elicited a moderate increase in IL-6 and IL-8 production. In contrast, Fusobacterium nucleatum provoked a substantial increase in IL-6 and IL-8 production. Interestingly, the gingival CTEs secreted significantly higher IL-6, while periodontal counterparts produced higher IL-8. In conclusion, the gingival and periodontal CTEs exhibited differential responses to various bacterial challenges. This gives insights into the contribution of tissue topography and fibroblast heterogeneity in rendering protective and specific immune responses toward early biofilm colonizers.https://doi.org/10.1177/20417314221111650 |
| spellingShingle | Hardik Makkar Srividya Atkuru Yi Ling Tang Tanya Sethi Chwee Teck Lim Kai Soo Tan Gopu Sriram Differential immune responses of 3D gingival and periodontal connective tissue equivalents to microbial colonization Journal of Tissue Engineering |
| title | Differential immune responses of 3D gingival and periodontal connective tissue equivalents to microbial colonization |
| title_full | Differential immune responses of 3D gingival and periodontal connective tissue equivalents to microbial colonization |
| title_fullStr | Differential immune responses of 3D gingival and periodontal connective tissue equivalents to microbial colonization |
| title_full_unstemmed | Differential immune responses of 3D gingival and periodontal connective tissue equivalents to microbial colonization |
| title_short | Differential immune responses of 3D gingival and periodontal connective tissue equivalents to microbial colonization |
| title_sort | differential immune responses of 3d gingival and periodontal connective tissue equivalents to microbial colonization |
| url | https://doi.org/10.1177/20417314221111650 |
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