Evaluation of expression and function of the H<sup>+</sup>/<it>myo</it>-inositol transporter HMIT
<p>Abstract</p> <p>Background</p> <p>The phosphoinositide (PIns) signalling pathway regulates a series of neuronal processes, such as neurotransmitter release, that are thought to be altered in mood disorders. Furthermore, mood-stabilising drugs have been shown to inhib...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2009-07-01
|
| Series: | BMC Cell Biology |
| Online Access: | http://www.biomedcentral.com/1471-2121/10/54 |
| _version_ | 1818272322905178112 |
|---|---|
| author | Shah Ajit J Langmead Christopher J Pell Theresa J Caberlotto Laura L Zambello Erika Mutinelli Chiara Mead Emma Mok Man HS Di Daniel Elena Duddy Graham Kew James NC Maycox Peter R |
| author_facet | Shah Ajit J Langmead Christopher J Pell Theresa J Caberlotto Laura L Zambello Erika Mutinelli Chiara Mead Emma Mok Man HS Di Daniel Elena Duddy Graham Kew James NC Maycox Peter R |
| author_sort | Shah Ajit J |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>The phosphoinositide (PIns) signalling pathway regulates a series of neuronal processes, such as neurotransmitter release, that are thought to be altered in mood disorders. Furthermore, mood-stabilising drugs have been shown to inhibit key enzymes that regulate PIns production and alter neuronal growth cone morphology in an inositol-reversible manner. Here, we describe analyses of expression and function of the recently identified H<sup>+</sup>/<it>myo</it>-inositol transporter (HMIT) investigated as a potential regulator of PIns signalling.</p> <p>Results</p> <p>We show that HMIT is primarily a neuronal transporter widely expressed in the rat and human brain, with particularly high levels in the hippocampus and cortex, as shown by immunohistochemistry. The transporter is localised at the Golgi apparatus in primary cultured neurones. No HMIT-mediated electrophysiological responses were detected in rat brain neurones or slices; in addition, inositol transport and homeostasis were unaffected in HMIT targeted null-mutant mice.</p> <p>Conclusion</p> <p>Together, these data do not support a role for HMIT as a neuronal plasma membrane inositol transporter, as previously proposed. However, we observed that HMIT can transport inositol triphosphate, indicating unanticipated intracellular functions for this transporter that may be relevant to mood control.</p> |
| first_indexed | 2024-12-12T21:40:14Z |
| format | Article |
| id | doaj.art-51f50de772494b71be0d9e47602b9224 |
| institution | Directory Open Access Journal |
| issn | 1471-2121 |
| language | English |
| last_indexed | 2024-12-12T21:40:14Z |
| publishDate | 2009-07-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Cell Biology |
| spelling | doaj.art-51f50de772494b71be0d9e47602b92242022-12-22T00:11:04ZengBMCBMC Cell Biology1471-21212009-07-011015410.1186/1471-2121-10-54Evaluation of expression and function of the H<sup>+</sup>/<it>myo</it>-inositol transporter HMITShah Ajit JLangmead Christopher JPell Theresa JCaberlotto Laura LZambello ErikaMutinelli ChiaraMead EmmaMok Man HSDi Daniel ElenaDuddy GrahamKew James NCMaycox Peter R<p>Abstract</p> <p>Background</p> <p>The phosphoinositide (PIns) signalling pathway regulates a series of neuronal processes, such as neurotransmitter release, that are thought to be altered in mood disorders. Furthermore, mood-stabilising drugs have been shown to inhibit key enzymes that regulate PIns production and alter neuronal growth cone morphology in an inositol-reversible manner. Here, we describe analyses of expression and function of the recently identified H<sup>+</sup>/<it>myo</it>-inositol transporter (HMIT) investigated as a potential regulator of PIns signalling.</p> <p>Results</p> <p>We show that HMIT is primarily a neuronal transporter widely expressed in the rat and human brain, with particularly high levels in the hippocampus and cortex, as shown by immunohistochemistry. The transporter is localised at the Golgi apparatus in primary cultured neurones. No HMIT-mediated electrophysiological responses were detected in rat brain neurones or slices; in addition, inositol transport and homeostasis were unaffected in HMIT targeted null-mutant mice.</p> <p>Conclusion</p> <p>Together, these data do not support a role for HMIT as a neuronal plasma membrane inositol transporter, as previously proposed. However, we observed that HMIT can transport inositol triphosphate, indicating unanticipated intracellular functions for this transporter that may be relevant to mood control.</p>http://www.biomedcentral.com/1471-2121/10/54 |
| spellingShingle | Shah Ajit J Langmead Christopher J Pell Theresa J Caberlotto Laura L Zambello Erika Mutinelli Chiara Mead Emma Mok Man HS Di Daniel Elena Duddy Graham Kew James NC Maycox Peter R Evaluation of expression and function of the H<sup>+</sup>/<it>myo</it>-inositol transporter HMIT BMC Cell Biology |
| title | Evaluation of expression and function of the H<sup>+</sup>/<it>myo</it>-inositol transporter HMIT |
| title_full | Evaluation of expression and function of the H<sup>+</sup>/<it>myo</it>-inositol transporter HMIT |
| title_fullStr | Evaluation of expression and function of the H<sup>+</sup>/<it>myo</it>-inositol transporter HMIT |
| title_full_unstemmed | Evaluation of expression and function of the H<sup>+</sup>/<it>myo</it>-inositol transporter HMIT |
| title_short | Evaluation of expression and function of the H<sup>+</sup>/<it>myo</it>-inositol transporter HMIT |
| title_sort | evaluation of expression and function of the h sup sup it myo it inositol transporter hmit |
| url | http://www.biomedcentral.com/1471-2121/10/54 |
| work_keys_str_mv | AT shahajitj evaluationofexpressionandfunctionofthehsupsupitmyoitinositoltransporterhmit AT langmeadchristopherj evaluationofexpressionandfunctionofthehsupsupitmyoitinositoltransporterhmit AT pelltheresaj evaluationofexpressionandfunctionofthehsupsupitmyoitinositoltransporterhmit AT caberlottolaural evaluationofexpressionandfunctionofthehsupsupitmyoitinositoltransporterhmit AT zambelloerika evaluationofexpressionandfunctionofthehsupsupitmyoitinositoltransporterhmit AT mutinellichiara evaluationofexpressionandfunctionofthehsupsupitmyoitinositoltransporterhmit AT meademma evaluationofexpressionandfunctionofthehsupsupitmyoitinositoltransporterhmit AT mokmanhs evaluationofexpressionandfunctionofthehsupsupitmyoitinositoltransporterhmit AT didanielelena evaluationofexpressionandfunctionofthehsupsupitmyoitinositoltransporterhmit AT duddygraham evaluationofexpressionandfunctionofthehsupsupitmyoitinositoltransporterhmit AT kewjamesnc evaluationofexpressionandfunctionofthehsupsupitmyoitinositoltransporterhmit AT maycoxpeterr evaluationofexpressionandfunctionofthehsupsupitmyoitinositoltransporterhmit |