Retinol kinetics in unsupplemented and vitamin A-retinoic acid supplemented neonatal rats: a preliminary model
Vitamin A (VA) metabolism in neonates is virtually uncharacterized. Our objective was to develop a compartmental model of VA metabolism in unsupplemented and VA-supplemented neonatal rats. On postnatal day 4, pups (n = 3/time) received 11,12-[3H]retinol orally, in either oil (control) or VA combined...
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| Format: | Article |
| Language: | English |
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Elsevier
2014-06-01
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| Series: | Journal of Lipid Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520352718 |
| _version_ | 1819293726169628672 |
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| author | Libo Tan Amanda E. Wray Michael H. Green A. Catharine Ross |
| author_facet | Libo Tan Amanda E. Wray Michael H. Green A. Catharine Ross |
| author_sort | Libo Tan |
| collection | DOAJ |
| description | Vitamin A (VA) metabolism in neonates is virtually uncharacterized. Our objective was to develop a compartmental model of VA metabolism in unsupplemented and VA-supplemented neonatal rats. On postnatal day 4, pups (n = 3/time) received 11,12-[3H]retinol orally, in either oil (control) or VA combined with retinoic acid (VARA) [VA (∼6 mg/kg body weight) + 10% retinoic acid]. Plasma and tissues were collected at 14 time points up to 14 days after dose administration. VARA supplementation rapidly, but transiently, increased total retinol mass in plasma, liver, and lung. It decreased the peak fraction of the dose in plasma. A multi-compartmental model developed to fit plasma [3H]retinol data predicted more extensive recycling of retinol between plasma and tissues in neonates compared with that reported in adults (144 vs. 12–13 times). In VARA pups, the recycling number for retinol between plasma and tissues (100 times) and the time that retinol spent in plasma were both lower compared with controls; VARA also stimulated the uptake of plasma VA into extravascular tissues. A VARA perturbation model indicated that the effect of VARA in stimulating VA uptake into tissues in neonates is both dramatic and transient. |
| first_indexed | 2024-12-24T04:15:00Z |
| format | Article |
| id | doaj.art-51f82a55650a44c9bb2ec385826bfdae |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-24T04:15:00Z |
| publishDate | 2014-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-51f82a55650a44c9bb2ec385826bfdae2022-12-21T17:15:57ZengElsevierJournal of Lipid Research0022-22752014-06-0155610771086Retinol kinetics in unsupplemented and vitamin A-retinoic acid supplemented neonatal rats: a preliminary modelLibo Tan0Amanda E. Wray1Michael H. Green2A. Catharine Ross3Graduate Program in Nutrition,Pennsylvania State University, University Park, PA 16802; Department of Nutritional Sciences, Pennsylvania State University, University Park, PA 16802Department of Nutritional Sciences, Pennsylvania State University, University Park, PA 16802Department of Nutritional Sciences, Pennsylvania State University, University Park, PA 16802To whom correspondence should be addressed; Department of Nutritional Sciences, Pennsylvania State University, University Park, PA 16802; Huck Institutes for the Life Sciences, Pennsylvania State University, University Park, PA 16802; To whom correspondence should be addressedVitamin A (VA) metabolism in neonates is virtually uncharacterized. Our objective was to develop a compartmental model of VA metabolism in unsupplemented and VA-supplemented neonatal rats. On postnatal day 4, pups (n = 3/time) received 11,12-[3H]retinol orally, in either oil (control) or VA combined with retinoic acid (VARA) [VA (∼6 mg/kg body weight) + 10% retinoic acid]. Plasma and tissues were collected at 14 time points up to 14 days after dose administration. VARA supplementation rapidly, but transiently, increased total retinol mass in plasma, liver, and lung. It decreased the peak fraction of the dose in plasma. A multi-compartmental model developed to fit plasma [3H]retinol data predicted more extensive recycling of retinol between plasma and tissues in neonates compared with that reported in adults (144 vs. 12–13 times). In VARA pups, the recycling number for retinol between plasma and tissues (100 times) and the time that retinol spent in plasma were both lower compared with controls; VARA also stimulated the uptake of plasma VA into extravascular tissues. A VARA perturbation model indicated that the effect of VARA in stimulating VA uptake into tissues in neonates is both dramatic and transient.http://www.sciencedirect.com/science/article/pii/S0022227520352718neonateliverlungcompartmental modelWinSAAMrecycling |
| spellingShingle | Libo Tan Amanda E. Wray Michael H. Green A. Catharine Ross Retinol kinetics in unsupplemented and vitamin A-retinoic acid supplemented neonatal rats: a preliminary model Journal of Lipid Research neonate liver lung compartmental model WinSAAM recycling |
| title | Retinol kinetics in unsupplemented and vitamin A-retinoic acid supplemented neonatal rats: a preliminary model |
| title_full | Retinol kinetics in unsupplemented and vitamin A-retinoic acid supplemented neonatal rats: a preliminary model |
| title_fullStr | Retinol kinetics in unsupplemented and vitamin A-retinoic acid supplemented neonatal rats: a preliminary model |
| title_full_unstemmed | Retinol kinetics in unsupplemented and vitamin A-retinoic acid supplemented neonatal rats: a preliminary model |
| title_short | Retinol kinetics in unsupplemented and vitamin A-retinoic acid supplemented neonatal rats: a preliminary model |
| title_sort | retinol kinetics in unsupplemented and vitamin a retinoic acid supplemented neonatal rats a preliminary model |
| topic | neonate liver lung compartmental model WinSAAM recycling |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520352718 |
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