Melatonin Promotes Accumulation of Resveratrol and Its Derivatives through Upregulation of <i>PAL</i>, <i>4CL</i>, <i>C4H</i>, and <i>STS</i> in Grape Seeds
Melatonin’s role in regulating the biosynthesis of phenolic compounds, particularly stilbenes, remains unknown in grape seeds. In this study, widely targeted metabolomics analysis revealed that 82 phenolic compounds were differentially accumulated in grape seeds from the berries treated with melaton...
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MDPI AG
2024-01-01
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| Series: | Horticulturae |
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| Online Access: | https://www.mdpi.com/2311-7524/10/1/65 |
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| author | Shiwei Gao Dezheng Wei Dandan Pan Fei Wang Hui Kang Yuxin Yao |
| author_facet | Shiwei Gao Dezheng Wei Dandan Pan Fei Wang Hui Kang Yuxin Yao |
| author_sort | Shiwei Gao |
| collection | DOAJ |
| description | Melatonin’s role in regulating the biosynthesis of phenolic compounds, particularly stilbenes, remains unknown in grape seeds. In this study, widely targeted metabolomics analysis revealed that 82 phenolic compounds were differentially accumulated in grape seeds from the berries treated with melatonin. The accumulation of resveratrol and its 11 derivatives was largely increased in melatonin-treated berry seeds. Additionally, melatonin treatment of preveraison grape berries increased the resveratrol content in seeds during berry ripening. The results of RNA-Seq showed that a total of 399 genes were differentially expressed, which were mainly involved in the plant hormone signal transduction, metabolic, and biosynthesis of secondary metabolites pathways, in melatonin-treated berry seeds compared to control seeds. Additionally, five <i>PALs</i>, four <i>4CLs</i>, one <i>C4H</i>, and four <i>STSs</i> were elucidated to be induced by melatonin in seeds at different time points after treatment. Therefore, melatonin promotes resveratrol accumulation and its derivatives, probably by upregulating the genes in the phenylpropanoid pathway, which provides precursors for resveratrol biosynthesis, and the <i>STS</i> genes in grape seeds. |
| first_indexed | 2024-03-08T10:49:11Z |
| format | Article |
| id | doaj.art-51fa244a150b4b829e8bbad077cf4764 |
| institution | Directory Open Access Journal |
| issn | 2311-7524 |
| language | English |
| last_indexed | 2024-03-08T10:49:11Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Horticulturae |
| spelling | doaj.art-51fa244a150b4b829e8bbad077cf47642024-01-26T16:49:51ZengMDPI AGHorticulturae2311-75242024-01-011016510.3390/horticulturae10010065Melatonin Promotes Accumulation of Resveratrol and Its Derivatives through Upregulation of <i>PAL</i>, <i>4CL</i>, <i>C4H</i>, and <i>STS</i> in Grape SeedsShiwei Gao0Dezheng Wei1Dandan Pan2Fei Wang3Hui Kang4Yuxin Yao5Key Laboratory of Biology and Genetic Improvement of Horticultural Crops in Huang-Huai Region, Ministry of Agriculture, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an 271018, ChinaKey Laboratory of Biology and Genetic Improvement of Horticultural Crops in Huang-Huai Region, Ministry of Agriculture, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an 271018, ChinaKey Laboratory of Biology and Genetic Improvement of Horticultural Crops in Huang-Huai Region, Ministry of Agriculture, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an 271018, ChinaKey Laboratory of Biology and Genetic Improvement of Horticultural Crops in Huang-Huai Region, Ministry of Agriculture, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an 271018, ChinaKey Laboratory of Biology and Genetic Improvement of Horticultural Crops in Huang-Huai Region, Ministry of Agriculture, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an 271018, ChinaKey Laboratory of Biology and Genetic Improvement of Horticultural Crops in Huang-Huai Region, Ministry of Agriculture, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an 271018, ChinaMelatonin’s role in regulating the biosynthesis of phenolic compounds, particularly stilbenes, remains unknown in grape seeds. In this study, widely targeted metabolomics analysis revealed that 82 phenolic compounds were differentially accumulated in grape seeds from the berries treated with melatonin. The accumulation of resveratrol and its 11 derivatives was largely increased in melatonin-treated berry seeds. Additionally, melatonin treatment of preveraison grape berries increased the resveratrol content in seeds during berry ripening. The results of RNA-Seq showed that a total of 399 genes were differentially expressed, which were mainly involved in the plant hormone signal transduction, metabolic, and biosynthesis of secondary metabolites pathways, in melatonin-treated berry seeds compared to control seeds. Additionally, five <i>PALs</i>, four <i>4CLs</i>, one <i>C4H</i>, and four <i>STSs</i> were elucidated to be induced by melatonin in seeds at different time points after treatment. Therefore, melatonin promotes resveratrol accumulation and its derivatives, probably by upregulating the genes in the phenylpropanoid pathway, which provides precursors for resveratrol biosynthesis, and the <i>STS</i> genes in grape seeds.https://www.mdpi.com/2311-7524/10/1/65melatoningrape seedstargeted metabolomicsRNA-Seqresveratrol |
| spellingShingle | Shiwei Gao Dezheng Wei Dandan Pan Fei Wang Hui Kang Yuxin Yao Melatonin Promotes Accumulation of Resveratrol and Its Derivatives through Upregulation of <i>PAL</i>, <i>4CL</i>, <i>C4H</i>, and <i>STS</i> in Grape Seeds Horticulturae melatonin grape seeds targeted metabolomics RNA-Seq resveratrol |
| title | Melatonin Promotes Accumulation of Resveratrol and Its Derivatives through Upregulation of <i>PAL</i>, <i>4CL</i>, <i>C4H</i>, and <i>STS</i> in Grape Seeds |
| title_full | Melatonin Promotes Accumulation of Resveratrol and Its Derivatives through Upregulation of <i>PAL</i>, <i>4CL</i>, <i>C4H</i>, and <i>STS</i> in Grape Seeds |
| title_fullStr | Melatonin Promotes Accumulation of Resveratrol and Its Derivatives through Upregulation of <i>PAL</i>, <i>4CL</i>, <i>C4H</i>, and <i>STS</i> in Grape Seeds |
| title_full_unstemmed | Melatonin Promotes Accumulation of Resveratrol and Its Derivatives through Upregulation of <i>PAL</i>, <i>4CL</i>, <i>C4H</i>, and <i>STS</i> in Grape Seeds |
| title_short | Melatonin Promotes Accumulation of Resveratrol and Its Derivatives through Upregulation of <i>PAL</i>, <i>4CL</i>, <i>C4H</i>, and <i>STS</i> in Grape Seeds |
| title_sort | melatonin promotes accumulation of resveratrol and its derivatives through upregulation of i pal i i 4cl i i c4h i and i sts i in grape seeds |
| topic | melatonin grape seeds targeted metabolomics RNA-Seq resveratrol |
| url | https://www.mdpi.com/2311-7524/10/1/65 |
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